Nadia , Salsabila (2026) UJI VIABILITAS DAN AKTIVITAS ANTAGONIS JAMUR AGEN HAYATI DARI PRODUK BIOFUNGISIDA TERHADAP Fusarium oxysporum SECARA IN VITRO DAN IN VIVO. FAKULTAS PERTANIAN, UNIVERSITAS LAMPUNG.
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Abstract
Biofungisida berbahan aktif jamur agen hayati merupakan alternatif pengendalian penyakit tanaman yang ramah lingkungan sebagai pengganti fungisida sintetis. Biofungisida ini memanfaatkan jamur antagonis seperti Trichoderma sp. dan Gliocladium sp. yang mampu menghambat perkembangan patogen. Penelitian ini bertujuan untuk menguji viabilitas dan efektivitas jamur agen hayati dari produk biofungisida terhadap F. oxysporum secara in vitro dan in vivo. Penelitian dilakukan dalam empat tahapan, yaitu: (1) karakterisasi morfologi jamur agen hayati yang terkandung pada produk biofungisida di media PDA, (2) uji viabilitas jamur agen hayati pada produk biofungisida, (3) uji efektivitas jamur agen hayati pada produk biofungisida terhadap F. oxysporum secara in vitro, dan (4) uji efektivitas jamur agen hayati pada produk biofungisida terhadap F. oxysporum secara in vivo. Perlakuan dalam penelitian ini disusun dalam rancangan acak lengkap (RAL), dengan tiga ulangan. Hasil penelitian menunjukkan bahwa kedua jamur agen hayati memiliki viabilitas yang hampir mencapai 100%. Trichoderma sp. memiliki kerapatan spora sebesar 4,08 × 10⁸ spora/mL dengan persentase perkecambahan 85,46%, sedangkan Gliocladium sp. sebesar 5,34 × 10⁸ spora/mL dengan persentase perkecambahan 92,57%. Pada uji in vitro, metode dual culture Gliocladium sp. menunjukkan daya hambat lebih tinggi 35,16% terhadap F. oxysporum dibandingkan Trichoderma sp. 21,50%. Pada metode double dish system Gliocladium sp. menunjukkan daya hambat yang lebih tinggi 50,83% dibandingkan Trichoderma sp. 48,70% dalam menghambat F. oxysporum. Namun, pada pengujian in vivo tampak bahwa kedua biofungisida tidak berpengaruh nyata terhadap keterjadian dan keparahan penyakit. Kata kunci: antagonis, biofungisida, F. oxysporum, Gliocladium sp., Trichoderma sp., viabilitas Biofungicides containing biocontrol fungi are environmentally friendly alternatives to synthetic fungicides for managing plant diseases. These biofungicides utilize antagonistic fungi, such as Trichoderma sp. and Gliocladium sp., to suppress the growth of plant pathogens. This study aimed to evaluate the viability and antagonistic activity of biocontrol fungi from commercial biofungicide products against Fusarium oxysporum under in vitro and in vivo conditions. The study consisted of four stages: (1) morphologicalcharacterization of biocontrol fungi on Potato Dextrose Agar (PDA), (2) viability testing, (3) evaluation of antagonistic activity against F. oxysporum in vitro , and (4) evaluation of effectiveness against F. oxysporum in vivo . The experiment was arranged in a Completely Randomized Design (CRD) with three replications. Both biocontrol fungi exhibited viability close to 100%. Trichoderma sp. had a spore density of 4,08 × 10⁸ spores mLwith 85,46% germination, whereas Gliocladium sp. had a spore density of 5.34 × 10⁸ spores mL with 92,57% germination. In the in vitro assay, Gliocladium sp. exhibited a higher inhibition rate against F. oxysporum than Trichoderma sp. in the dual culture method, with inhibition percentages of 35,16% and 21,50%, respectively. Similarly, in the double dish system method, Gliocladium sp. showed a slightly higher inhibition rate 50,83% than Trichoderma sp. 48,70% in suppressing the growth of F. oxysporum. However, under in vivo conditions, neither biofungicide significantly affected disease incidence or disease severity. Keywords: antagonism, biofungicide, Fusarium oxysporum , Gliocladium sp., Trichoderma sp., viability.
| Item Type: | Other |
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| Subjects: | ?? 630 ?? |
| Divisions: | ?? proteksi ?? |
| Depositing User: | 2606977866 Digilib |
| Date Deposited: | 10 Jul 2026 02:01 |
| Last Modified: | 10 Jul 2026 02:01 |
| URI: | http://digilib.unila.ac.id/id/eprint/102376 |
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