STUDI PENGARUH PENAMBAHAN GLISEROL DAN SORBITOL TERHADAP STABILITAS ENZIM SELULASE DARI Aspergillus niger L-51

0817011007, Ni Putu Yuliastri (2012) STUDI PENGARUH PENAMBAHAN GLISEROL DAN SORBITOL TERHADAP STABILITAS ENZIM SELULASE DARI Aspergillus niger L-51. Digital Library.

[img]
Preview
FIle PDF
bab 1.pdf

Download (20Kb) | Preview
[img]
Preview
FIle PDF
bab 2.pdf

Download (183Kb) | Preview
[img]
Preview
FIle PDF
bab 3.pdf

Download (45Kb) | Preview
[img]
Preview
FIle PDF
1. COVER.pdf

Download (21Kb) | Preview
[img]
Preview
FIle PDF
2. ABSTRAK - inggris.pdf

Download (12Kb) | Preview
[img]
Preview
FIle PDF
3. ABSTRAK.pdf

Download (10Kb) | Preview
[img]
Preview
FIle PDF
4. COVER DALAM.pdf

Download (17Kb) | Preview
[img]
Preview
FIle PDF
5. LEMBAR PENGESAHAN.pdf

Download (5Kb) | Preview
[img]
Preview
FIle PDF
6. LEMBAR MENGESAHKAN.pdf

Download (7Kb) | Preview
[img]
Preview
FIle PDF
7. RIWAYAT HIDUP.pdf

Download (13Kb) | Preview
[img]
Preview
FIle PDF
8. Kata Mutiara.pdf

Download (32Kb) | Preview
[img]
Preview
FIle PDF
9. Persembahan.pdf

Download (45Kb) | Preview
[img] FIle PDF
12. Bab 4.pdf
Restricted to Hanya pengguna terdaftar

Download (260Kb)
[img]
Preview
FIle PDF
13. Bab 5.pdf

Download (9Kb) | Preview

Abstrak

Abstrak Enzim selulase merupakan enzim yang mampu mengkonversi selulosa menjadi glukosa. Dalam proses industri, enzim ini harus stabil pada pH dan suhu ekstrim. Oleh sebab itu dilakukan penambahan gliserol dan sorbitol terhadap enzim selulase dari Aspergillus niger L-51 untuk mempelajari pengaruhnya terhadap stabilitas enzim selulase. Untuk mencapai tujuan tersebut, dilakukan produksi, isolasi, pemekatan, dan pemurnian enzim, serta karakterisasi enzim selulase hasil pemurnian sebelum dan setelah penambahan gliserol dan sorbitol. Hasil penelitian menunjukkan bahwa aktivitas spesifik enzim selulase hasil pemurnian 5,611 U/mg, meningkat 5 kali dibandingkan dengan ekstrak kasar enzim yang mempunyai aktivitas spesifik 1,115 U/mg. Enzim hasil pemurnian sebelum dan setelah penambahan gliserol dan sorbitol memiliki pH optimum 4,5 dan suhu optimum 50°C. Data kinetika menunjukkan enzim hasil pemurnian memiliki KM = 24,20 mg/mL substrat dan Vmaks = 3,75 μmol/mL.menit. Enzim setelah penambahan gliserol 0,5; 1; dan 1,5 M memiliki KM yaitu 20,07 mg/mL; 18,54 mg/mL; dan 19,44 mg/mL; dan Vmaks yaitu 3,4 μmol/mL.menit; 3,3 μmol/mL.menit; dan 3,36 μmol/mL.menit. Sedangkan enzim setelah penambahan sorbitol 0,5; 1; dan 1,5 M memiliki KM yaitu 17,56 mg/mL; 16,19 mg/mL; dan 17,1 mg/mL; dan Vmaks: 3,07 μmol/mL.menit; 2,97 μmol/mL.menit; dan 3,04 μmol/mL.menit. Uji stabilitas termal enzim hasil pemurnian selama 100 menit pada 50oC menunjukkan nilai ki = 0,0119 menit-1; t1/2 = 58,24 menit dan ΔGi yaitu 102,24 kJ/mol. Enzim setelah penambahan gliserol 0,5; 1; dan 1,5 M memiliki ki yaitu 0,0083; 0,0076; dan 0,0078 menit-1; t1/2: 83,49; 91,18 dan 88,85 menit; serta ΔGi: 103,21 kJ/mol; 103,44 kJ/mol; dan 103,37 kJ/mol. Sedangkan enzim setelah penambahan sorbitol 0,5; 1; dan 1,5 M memiliki ki yaitu 0,0082; 0,0075; dan 0,0077 menit-1; t1/2: 84,51; 92,4 dan 90 menit; serta ΔGi : 103,24 kJ/mol; 103,48 kJ/mol; dan 103,41 kJ/mol. Penambahan gliserol dan sorbitol terhadap enzim selulase dari Aspergillus niger L-51 dapat meningkatkan stabilitas termal enzim yang dilihat melalui peningkatan waktu paruh dan ΔGi serta penurunan nilai ki. Kata kunci : Selulase, gliserol, sorbitol, Aspergillus niger L-51 Abstract Cellulase is enzyme which has important part in bioconversion process of cellulose into glucose and often used in many industrial processes both for relating to food and non food. In industrial process, this enzyme must be able to work in extreme pH and temperature. But generally this enzyme is unstable at that condition. This research was aimed to study the effect of addition glycerol and sorbitol toward stability of cellulase from Aspergillus niger L-51. Sequential experiment, starting from the enzyme production, isolation, purification, and characterization before and after addition glycerol and sorbitol. The result showed that the purified enzyme has a specific activity 5,611 U/mg, increase at 5 times than native enzyme which has specific activity 1,115 U/mg. The purified enzyme before and after addition glycerol and sorbitol have optimum pH 4,5 and optimum temperature 50oC. The kinetics data showed that purified enzyme has KM = 15,21 mg/mL and Vmax = 2,81 μmol/mL.min. Enzyme after addition glycerol 0,5; 1; and 1,5 M have KM with following data: 20,07 mg/mL; 18,54 mg/mL; and 19,44 mg/mL and Vmax were shown with following data: 3,40 μmol/mL.min; 3,30 μmol/mL.min and 3,36 μmol/mL.min. Enzyme after addition sorbitol 0,5; 1; and 1,5 M have KM with following data: 17,56 mg/mL; 16,19 mg/mL; and 17,1 mg/mL and Vmax were shown with following data: 3,07 μmol/mL.min; 2,97 μmol/mL.min; and 3,04 μmol/mL.min. Thermal stability of purified enzyme stored for 100 min at 50oC were shown by the values ki = 0,0119 min-1; t1/2 = 58,24 min and ΔGi = 102,24 kJ/mol. Enzyme after addition glycerol 0,5; 1; and 1,5 M have ki value= 0,0083; 0,0076; and 0,0078 min-1; t1/2= 83,49; 91,18 and 88,85 min; ΔGi = 103,21 kJ/mol; 103,44 kJ/mol; and 103,37 kJ/mol. While enzyme after addition sorbitol 0,5; 1; and 1,5 M have ki value= 0,0082; 0,0075; and 0,0077 min-1; t1/2 = 84,51; 92,4 and 90 min; ΔGi = 103,24 kJ/mol; 103,48 kJ/mol; and 103,41 kJ/mol. Addition glycerol and sorbitol on cellulase from Aspergillus niger L-51 can increase the thermal stability of enzyme based on the decrease of ki value and the increase of t1/2 and ΔGi. Key words: Cellulase, glycerol, sorbitol, Aspergillus niger L-51

Jenis Karya Akhir: Artikel
Subyek:
Program Studi: Fakultas MIPA > Prodi Biologi
Pengguna Deposit: IC-STAR . 2015
Date Deposited: 22 Oct 2015 08:56
Terakhir diubah: 22 Oct 2015 08:56
URI: http://digilib.unila.ac.id/id/eprint/13385

Actions (login required)

Lihat Karya Akhir Lihat Karya Akhir