Anwika Utami Putri Djuardi, 1114051006 (2015) OPTIMASI HIDROLISIS JERAMI PADI UNTUK PRODUKSI BIOETANOL DENGAN METODE SIMULTANEOUS SACCHARIFICATION AND FERMENTATION. FAKULTAS PERTANIAN, UNIVERSITAS LAMPUNG.
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Abstrak (Berisi Bastraknya saja, Judul dan Nama Tidak Boleh di Masukan)
Jerami padi merupakan limbah padat agroindustri yang memiliki komponen lignoselulosa tinggi dan mempunyai potensi untuk dijadikan bioetanol. Salah satu metode yang dapat digunakan untuk pembuatan bioetanol dari jerami padi ialah Simultaneous Saccharification and Fermentation (SSF). SSF merupakan metode yang melakukan tahapan hidrolisis dan tahapan fermentasi pada tempat dan waktu yang sama, sehingga proses konversinya berjalan lebih efektif dan efisien. Tujuan dari penelitian ini adalah mendapatkan konsentrasi substrat dan konsentrasi enzim optimal yang menghasilkan gula reduksi (g/L) tertinggi pada proses hidrolisis serta mengetahui waktu prehidrolisis terbaik yang menghasilkan kadar etanol (g/L) tertinggi pada proses SSF. Penelitian ini dilakukan dua tahap yaitu tahap optimasi hidrolisis dan tahap SSF. Tahap optimasi hidrolisis terdiri dari 2 tahap, yaitu optimasi konsentrasi substrat (6; 8; 10 dan 12% (b/v)) dan optimasi konsentrasi enzim (20; 25; 30 dan 35 FPU/g selulosa). Konsentrasi substrat dan enzim optimal pada tahap optimasi hidrolisis ini selanjutnya akan digunakan pada tahap SSF. Sebelum dilakukannya tahap SSF, jerami padi dihidrolisis terlebih dahulu (prehidrolisis) selama beberapa waktu (0, 12, dan 24 jam) pada kondisi proses suhu 500C, pH 4,8, dan kecepatan shaker 150 rpm. Setelah prehidrolisis, kondisi suhu proses diturunkan menjadi 380C, masing-masing sampel ditambahkan 10% (v/v) Saccharomyces cerevisiae, kemudian sampel diinkubasi pada suhu 38oC, 150 rpm selama 72 jam. Hasil penelitian menunjukkan bahwa konsentrasi substrat 12% dan konsentrasi enzim 35 FPU/g selulosa merupakan konsentrasi optimal yang menghasilkan gula reduksi (g/L) tertinggi (37,33 g/L) pada proses hidrolisis. Perlakuan waktu prehidrolisis 0 jam (tanpa prehidrolisis) merupakan perlakuan yang menghasilkan kadar etanol tertinggi yaitu sebesar 16,19 g/L pada proses SSF jerami padi. Kata kunci: jerami padi, bioetanol, hidrolisis enzimatis, SSF, selulase. ABSTRACT HYDROLYSIS OPTIMIZATION OF RICE STRAW FOR BIOETHANOL PRODUCTION BY USING SIMULTANEOUS SACCHARIFICATION AND FERMENTATION METHOD Rice straw is an agro-industrial solid waste that contains high lignocellulosic material and has considerable potential as a raw material for bioethanol production. One of the methods for bioethanol production from rice straw is Simultaneous Saccharification and Fermentation (SSF). SSF is a method where the enzymatic hydrolysis and the microbial fermentation take place at the same place and time, so that the conversion at cellulosic material into ethanol will be more effective and efficience. The objective of this study were to determine the optimum concentration of substrate and enzyme cellulase that produce the highest sugar reduction concentration in hydrolysis process and to find out the best prehydrolysis time that produce highest ethanol concentration in SSF stage. This study was conducted in two stages, the first stage is enzymatic hydrolysis optimization and the second stage is SSF process. The enzymatic hydrolysis optimization stage was carried out in two steps, namely optimization of substrate concentration of rice straw (6; 8; 10 and 12% (w / v)) and optimization of enzyme celullase concentration (20; 25; 30 and 35 FPU/g cellulose). The optimum concentration of substrate and enzyme as resulted from this stage will be aplicated in SSF process. Prior to the SSF, an enzymatic prehydrolysis of rice straw carried out in different time periods (0, 12 and 24 h) and it performed on the condition of process at 500C, pH 4,8 and rotary shaker 150 rpm. After that, the temperature process was decreased to 380C and each sample was added with 10% (v/v) Saccharomyces cerevisiae, then the samples were incubated at 380C, 150 rpm for 72 hours. The results showed that the concentration of substrat 12 % (w/v) and the concentration of enzyme cellulase 35 FPU/g cellulose were the optimal concentration that produce the highest reducing sugars (37,33 g/L) in the 32 h hydrolysis incubation, and the treatment of prehydrolysis 0 h was a treatment that produce the highest ethanol concentration, 16,19 g/L, in the 72 h SSF process. Keywords: rice straw, bioethanol, hydrolysis enzymatic, SSF, cellulase.
| Jenis Karya Akhir: | Skripsi |
|---|---|
| Subyek: | > Pertanian ( Umum ) > Budidaya tanaman |
| Program Studi: | Fakultas Pertanian dan Pascasarjana > Prodi Teknologi Hasil Pertanian |
| Pengguna Deposit: | 8897090 . Digilib |
| Date Deposited: | 30 Oct 2015 08:20 |
| Terakhir diubah: | 30 Oct 2015 08:20 |
| URI: | http://digilib.unila.ac.id/id/eprint/14285 |
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