0617011017, Yulistia Anggraini (2012) PENAPISAN DAN KARAKTERISASI SENYAWA ANTIOKSIDAN ACTINOMYCETES YANG BERASOSIASI DENGAN SPONGA. Digital Library.
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Abstract
Abstrak Telah dilakukan penapisan dan karakterisasi senyawa antioksidan actinomycetes yang berasosiasi dengan sponga. Hasil penapisan awal memberikan informasi bahwa strain AT05 memiliki aktivitas antioksidan paling tinggi dibandingkan strain ANCb-6, ANT2, ANT3, dan ANTd3. Uji kromatografi lapis tipis (KLT) dengan pereaksi ninhidrin dan Dragendroff menunjukkan komponen antioksidan tersebut merupakan golongan alkaloid. Ekstrak AT05 kemudian dimurnikan melalui beberapa tahapan kromatografi sehingga diperoleh senyawa YA1 0,7 mg dan YA2 4,8 mg. Spektrum FTIR YA1 menunjukkan bahwa senyawa YA1 memiliki vibrasi tarik O-H pada daerah sekitar 3427 cm-1, vibrasi tarik C=C pada daerah 1680 cm-1, vibrasi tarik C-N pada daerah 1259 cm-1, dan puncak tunggal dari vibrasi tekuk N-H pada daerah 803 cm-1. Spektrum FTIR YA2 menunjukkan bahwa senyawa YA2 memiliki vibrasi tarik O-H pada daerah sekitar 3415 cm-1, vibrasi tarik C=C pada daerah 1681 cm-1, vibrasi tarik C-N pada daerah 1145 cm-1, dan vibrasi tekuk N-H pada daerah 1644 cm-1. Informasi tesebut mengindikasikan bahwa senyawa YA1 dan YA2 memiliki gugus hidroksi, ikatan rangkap, amina sekunder, dan N-tersier. Senyawa YA1 dan YA2, dalam kadar 1 mg/mL, secara berurutan memiliki kemampuan meredam reaksi radikal bebas DPPH sebesar 13% dan 29%. Berdasarkan informasi tersebut disimpulkan bahwa isolat AT05 mampu memproduksi senyawa metabolit sekunder golongan alkaloid yang bersifat sebagai antioksidan. Abstract The screening and characterization of antioxidant compounds from actinomycetes associated with sponges has been carried out. The result of the screening gave an information that strain AT05 had the highest antioxidant activity compared to strain ANCb-6, ANT2, ANT3, and ANTd3. The test of thin layer chromatography with ninhydrin and Dragendorff reagents showed that the antioxidant component is belong to alkaloid group. The extract of AT05 was purified by several steps of chromatography to get compounds YA1 0,7 mg and YA2 4,8 mg. The FTIR spectrum of YA1 showed that YA1 compound had O-H stretching vibration at 3427 cm-1, C=C stretching vibration at 1680 cm-1, C-N stretching vibration at 1259 cm-1, and a single band of N-H bending vibration at 803 cm-1. The FTIR spectrum of YA2 showed that YA2 compound had O-H stretching vibration at 3415 cm-1, C=C stretching vibration at 1681 cm-1, C-N stretching vibration at 1145 cm-1 and N-H bending vibration at 1644 cm-1. These data indicate that YA1 and YA2 belong to alkaloid group which have the hydroxyl group, double bond, secondary and tertiary amine groups. The scavenging ability of YA1 was 13 % and YA2 was 29% in 1 mg/mL. It concluded that isolate AT05 produce the secondary metabolite compounds of alkaloid group with an antioxidant activity
| Item Type: | Article |
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| Subjects: | A General Works = Karya Karya Umum |
| Divisions: | Fakultas MIPA > Prodi Kimia |
| Depositing User: | IC-STAR . 2015 |
| Date Deposited: | 20 Nov 2015 01:48 |
| Last Modified: | 20 Nov 2015 01:48 |
| URI: | http://digilib.unila.ac.id/id/eprint/14408 |
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