ISOLASI DAN KARAKTERISASI SENYAWA ANTOSIANIN DARI DAUN ADAM HAWA (Rhoeo discolor L. Her)

Junaidi Permana, 1117011027 (2015) ISOLASI DAN KARAKTERISASI SENYAWA ANTOSIANIN DARI DAUN ADAM HAWA (Rhoeo discolor L. Her). MIPA, Universitas Lampung.

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COVER LUAR.pdf

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ABSTRACT.pdf

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ABSTRAK.pdf

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COVER DALAM.pdf

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LEMBAR PERSETUJUAN.pdf

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LEMBAR PENGESAHAN.pdf

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RIWAYAT HIDUP.pdf

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MOTO.pdf

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PERSEMBAHAN.pdf

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SANWACANA.pdf

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DAFTAR ISI.pdf

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DAFTAR TABEL.pdf

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DAFTAR GAMBAR.pdf

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BAB I.pdf

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BAB II.pdf

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BAB V.pdf

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DAFTAR PUSTAKA.pdf

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LAMPIRAN-LAMPIRAN.pdf

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Abstrak

Anthocyanins has been isolated from adam hawa (Rhoeo discolor L. Her) leaves, using maceration method with water : HCl (100: 0.1 v/v) as a solvent. Extract was concentrated using freeze drying method, and it was separated on Sephadex LH-20 coloumn. Fraction 2-5 from elution showed one spot on TLC chromatogram using silica GF245 as adsorbent, and buthanol: acetic acid: water (4: 1: 5 v/v) as eluent at Rf 0.42. This fraction was analized with UV-Vis and it was found that the spot has maximum wavelength at 535.0 nm, and it predicted as cyanidin-3-galactose or peonidin-3-glucose. The compound was found to exhibit 80.23891% antioxidant activity based on DPPH (2,2-diphenyl-1-picrylhydrazil) method. Key Words : Anthocyanins, Rhoeo discolor L. Her, Sephadex LH-20, Antioxidant, and DPPH Antosianin telah diisolasi dari daun adam hawa (Rhoeo discolor L. Her), menggunakan metode maserasi dengan pelarut akuades : HCl (100: 0,1 v/v). Ekstrak dipekatkan dengan menggunakan metode freeze drying, kemudian dilakukan pemisahan menggunakan metode kromatografi kolom sefadeks LH-20. Hasil elusi pada fraksi 2-5 menunjukkan adanya 1 noda pada kromatogram KLT dengan menggunakan adsorben silika GF245, eluen butanol: asam asetat: air (4: 1: 5 v/v) pada Rf 0,42. Fraksi tersebut kemudian dianalisis menggunakan spektrofotometri UV-Vis, diperoleh panjang gelombang maksimal pada 534.0 nm, dan diperkirakan sebagai sianidin-3-galaktosa atau peonidin-3-glukosa. Fraksi antosianin hasil isolasi juga memiliki aktivitas antioksidan sebesar 80,23891% berdasarkan metode DPPH (2,2-difenil-1-pikrilhidrazil). Kata Kunci : Antosianin, Rhoeo discolor L. Her, Sefadeks LH-20, Antioksidan, dan DPPH.

Tipe Karya Ilmiah: Skripsi
Subyek: Q Science (General)
Program Studi: Fakultas MIPA > Prodi Kimia
Depositing User: 4353951 . Digilib
Date Deposited: 11 Dec 2015 03:29
Last Modified: 11 Dec 2015 03:29
URI: http://digilib.unila.ac.id/id/eprint/15591

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