PENINGKATAN KESTABILAN ENZIM LIPASE DARI Pseudomonas aeruginosa ATCC 27853 DENGAN AMOBILISASI MENGGUNAKAN BENTONIT

NOPIANI, 1327011031 (2015) PENINGKATAN KESTABILAN ENZIM LIPASE DARI Pseudomonas aeruginosa ATCC 27853 DENGAN AMOBILISASI MENGGUNAKAN BENTONIT. MIPA, Universitas Lampung.

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Abstrak

Pada penelitian ini telah dilakukan amobilisasi enzim lipase dari Pseudomonas aeruginosa ATCC 27853 menggunakan bentonit untuk meningkatkan kestabilan enzim. Tahapan prosedur yang dilakukan pada penelitian ini meliputi : kondisi pertumbuhan optimum, produksi, isolasi, pemurnian, amobilisasi dan karakterisasi enzim lipase hasil pemurnian sebelum dan sesudah amobilisasi. Aktivitas enzim lipase ditentukan dengan metode Titrimetri. Kadar protein ditentukan dengan metode Lowry. Hasil penelitian menunjukkan kondisi optimum pertumbuhan Pseudomonas aeruginosa ATCC 27853 menghasilkan enzim lipase dengan aktivitas tertinggi pada pH 7, temperatur 35°C dan waktu inkubasi selama 48 jam. Aktivitas spesifik enzim hasil pemurnian sebesar 520,000 U/mg, meningkat kemurniannya 9,2 kali dibandingkan ekstrak kasar enzim lipase dengan aktivitas spesifik sebesar 56,491 U/mg. Enzim lipase hasil pemurnian mempunyai suhu optimum 35°C, KM 86,177 mgmL-1 substrat, Vmaks 35,714 μmol mL-1 menit-1, ki = 0,023 menit-1, t1/2 = 30,130 menit, dan ΔGi = 95,669 kJ mol-1. Enzim lipase hasil amobilisasi mempunyai suhu optimum 35 °C, KM 4,742 mgmL-1 substrat, Vmaks 4,854 μmol mL-1 menit-1, ki = 0,018 menit-1, t1/2 = 38,500 menit, dan ΔGi = 96,296 kJ mol-1. Berdasarkan penelitian yang telah dilakukan diketahui bahwa amobilisasi enzim lipase menggunakan matriks bentonit dapat meningkatkan kestabilan enzim dibandingkan dengan enzim tanpa amobilisasi. Kata kunci : amobilisasi, bentonit, lipase, peningkatan kestabilan Pseudomonas aeruginosa ATCC 27853 Abstrak Bahasa Inggris The aim of this research is to increase the stability of lipase from Pseudomonas aeruginosa ATCC 27583 using immobilization method with bentonite. Several procedures were performed to achieve the aim, which were optimum growth condition, production, isolation, purification, immobilization and characterization of the purified enzyme before and after immobilization. Lipase enzyme activity was determined by Titrimetic method. Protein content was determined by Lowry method. The results showed that the optimum condition of P. aeruginosa ATCC 27583 to produce the lipase with the highest activity was at pH 7, temperature 35°C and incubation time for 48 hour. The specific activity of purified lipase was 520.000 U/mg, an increase of 9.2 times compared to that of the crude extract which has 56.491 U/mg. The characteristics of purified lipase were optimum temperature of 35°C; KM 86.177 mg/mL-1substrate; Vmax 35.714 μmol/mL-1min-1; ki = 0.023 min-1, t1/2 = 30.130 minutes, and ΔGi = 95.669 kJ mol-1. The characteristics of the immobillized lipase were optimum temperature of 35°C; KM-1 4.742 mg/mL-1 substrate; Vmax 4.854 μmol/mL-1 min-1 , ki = 0.018 min-1, t1/2 = 38.500 minutes, and ΔGi = 96.296 kJ mol-1. Immobilization using bentonite was able to increase the stability of lipase. Keywords: bentonite, immobilization, increased stability, lipase, P. aeruginosa ATCC 27853.

Tipe Karya Ilmiah: Skripsi
Subyek: Q Science (General)
Program Studi: Fakultas MIPA > Prodi Magister Ilmu Kimia
Depositing User: 4024407 . Digilib
Date Deposited: 12 Dec 2015 03:41
Last Modified: 12 Dec 2015 03:41
URI: http://digilib.unila.ac.id/id/eprint/15670

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