PENINGKATAN KESTABILAN ENZIM PROTEASE DARI Bacillus subtilis ITBCCB148 DENGAN AMOBILISASI MENGGUNAKAN KALSIUM ALGINAT

Nurhaeni, 1327011032 (2015) PENINGKATAN KESTABILAN ENZIM PROTEASE DARI Bacillus subtilis ITBCCB148 DENGAN AMOBILISASI MENGGUNAKAN KALSIUM ALGINAT. Masters thesis, Universitas Lampung.

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Abstrak

Penelitian ini bertujuan untuk meningkatkan stabilitas enzim protease dari isolat bakteri Bacillus subtilis ITBCCB148 melalui proses amobilisasi dengan metode penjebakan enzim (entrapment) menggunakan Ca-alginat. Protease adalah biokatalis yang dapat menghidrolisis protein menjadi asam amino dan banyak digunakan secara komersial dalam industri pangan dan non-pangan. Agar dapat digunakan dalam proses industri, maka enzim harus dapat bekerja pada pH dan suhu ekstrim. Untuk mencapai tujuan tersebut, maka dilakukan proses produksi, isolasi, pemurnian dan amobilisasi enzim hasil pemurnian. Hasil penelitian menunjukkan aktivitas spesifik enzim hasil pemurnian sebesar 999,372 U/mg, meningkat kemurniannya 17,540 kali dibandingkan ekstrak kasar enzim dengan perolehan 53,723 U/mg. Enzim ini memiliki suhu optimum 55ºC, harga KM = 10,000 mg mL-1 substrat dan harga Vmaks = 250,000 μmol mL-1 menit-1. Uji stabilitas termal pada suhu 60ºC selama 70 menit untuk enzim hasil pemurnian masih memiliki aktivitas sisa 4,514%, t1/2 = 15,400 menit, ki = 0,045 menit-1 dan ΔGi = 98,653 kJ mol-1. Enzim amobilisasi memiliki suhu optimum 60ºC, harga KM = 2,061 mg mL-1 substrat dan harga Vmaks = 4,695 μmol mL-1 menit-1. Uji stabilitas termal pada suhu 60ºC selama 70 menit masih memiliki aktivitas sisa 43,534%, t1/2 = 57,750 menit, ki = 0,012 menit-1 dan ΔGi = 105,453 kJ mol-1. Berdasarkan penurunan nilai ki terjadi peningkatan stabilitas termal hingga 3,750 kali. Kata kunci : Protease, Bacillus subtilis ITBCCB148, Amobilisasi enzim, Kalsium alginat Abstrak Bahasa Inggris The objective of this reserach was to improve protease enzyme stability from bacterial isolates of Bacillus subtilis ITBCCB148 with immobilization process by Ca-alginate. Protease is a bio-catalyst that is able to hydrolize protein into amino acid and protease used in processing industries. In order to be able to use in industrial process, the enzym should be able to work in extreme temperature. To obtain this objective, process of enzyme production, isolation, purification, and immobilization were conducted. The reserach results showed the enzyme specific activity as a result of purification was 999.372 U/mg, and the purity improved 17.540 folds than the crude extract. This enzyme has optimum temperature at 55°C with KM = 10. 000 mg/ml-1, Vmaks = 250.000 μmol mL-1 min-1. Then, the residu activity of thermal stability on 60°C during 70 minutes were 4,514%, t1/2 = 15.400 min, ki = 0.045 min-1 and ΔGi = 98.653 kJ mol-1. The amobil enzyme has an optimum reaction such as, optimum temperature at 60°C, KM = 2.601 mg/ml-1 and Vmaks = 4.695 μmol mL-1 min-1. Thermal stability of amobil enzyme were shown with following data, residu activity = 43.534 %, t1/2 = 57.750 min, ki = 0.012 min-1 and ΔGi = 105.453 kj mol-1 . Based on reduced value of ki , there was thermal stability improvement up to 3.750 folds. Keywords : Protease, Bacillus subtilis ITBCCB148, Enzyme immobilization, Calcium Alginate.

Tipe Karya Ilmiah: Tesis (Masters)
Subyek: Q Science (General) > QD Chemistry
Program Studi: Fakultas MIPA > Prodi Magister Ilmu Kimia
Depositing User: 6739606 . Digilib
Date Deposited: 12 Dec 2015 03:55
Last Modified: 12 Dec 2015 03:55
URI: http://digilib.unila.ac.id/id/eprint/15673

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