POLA RESISTENSI ANTIBIOTIK DAN PROFIL PLASMID BAKTERI Klebsiella sp. YANG BERPOTENSI SEBAGAI PENYEBAB INFEKSI NOSOKOMIAL DI RSUD ABDUL MOELOEK PROVINSI LAMPUNG

SYAMSINAR , 1327011036 (2015) POLA RESISTENSI ANTIBIOTIK DAN PROFIL PLASMID BAKTERI Klebsiella sp. YANG BERPOTENSI SEBAGAI PENYEBAB INFEKSI NOSOKOMIAL DI RSUD ABDUL MOELOEK PROVINSI LAMPUNG. Masters thesis, UNIVERSITAS LAMPUNG.

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Abstrak

ABSTRAK Resistensi antibiotik merupakan masalah kesehatan masyarakat yang utama di dunia. Klebsiella dilaporkan memiliki tingkat resistensi yang tinggi dan sebagai penyebab infeksi nosokomial di banyak negara. Penelitian ini bertujuan untuk mengetahui pola resistensi antibiotik dari Klebsiella sp. isolat dari rumah sakit umum Provinsi Lampung dan untuk menyelidiki hubungan antara pola resistensi dan profil plasmidnya. Isolat diperoleh dari darah, urin, sputum, dan nanah dari pasien rumah sakit. Tiga puluh dari 600 sampel menunjukkan signifikan sebagai Klebsiella sp. Isolat ini dilakukan uji kepekaan antibiotik metode difusi cakram Kirby-Bauer. Data-data isolat menunjukkan bahwa 83,3, 73,3, 63,3, 60, 56,7, 53,3, 50,0 dan 43,3% resisten terhadap Ampisilin, Amoksisilin-klavulanat, Seftriakson, Siprofloksasin Gentamisin, Sefotaksim. Septazidim, dan Kloramfenikol. Selanjutnya isolat dilakukan uji keberadaan plasmid dengan menggunakan Plasmid-Miniprep Kit dan Agarose Gel . Elektroforesis menunjukkan 13 isolat (43%) yang terdapat plasmid. Beberapa isolat memiliki plasmid tunggal sementara yang lain memiliki beberapa plasmid, dengan ukuran berkisar antara 2.4-9.9 kb. Sementara itu, analisis statistik menunjukkan bahwa ada korelasi yang signifikan antara profil plasmid dan pola resistensi antibiotik. Kata kunci : Infeksi nosokomial, Klebsiella sp., resistensi antibiotik, profil Plasmid ABSTRACT Antibiotic resistance is a major public health problem in the world. Klebsiella is reported as resistant strains of hospital-acquired infection in many countries. The study was aimed to determine the antibiotic resistance pattern of Klebsiella sp isolates from Lampung Province-general hospital and to investigate relationship between resistance pattern and its plasmid profiles. The isolates were obtained from blood, urine, sputum and pus of patients of the hospital. Thirty out of 600 specimens showed significant as Klebsiella sp. The isolates were subjected to antibiotic resistance-test by modified Kirby-bauer disc diffusion. The datas showed that the isolates were 83.3, 73.3, 63.3, 60, 56,7, 53,3, 50,0 and 43,3 % resistant toward Ampicillin, Amoxcillin-clavulanic, Cefriazone Ciprofloxacin, Gentamisin, Cefotaxime Ceptazidime, and Chloramphenicol, respectively. Further investigation on its plasmid contents by using PlasmidMiniprep Kit and Agarose Gel Electrophoresis showed 13 of the isolates (43%) contained plasmid. Several isolates possessed a single plasmid while the others possessed multiple plasmids, with size ranged between 2.4 - 9.9 kb. Meanwhile, statistical analysis showed that there were significant correlations between plasmid profiles and its antibiotics resistance patterns. Keywords : Nosocomial infection, Klebsiella sp., antibiotic-resistance, Plasmid profile

Tipe Karya Ilmiah: Tesis (Masters)
Subyek: Q Science (General)
Program Studi: Fakultas MIPA > Prodi Magister Ilmu Kimia
Depositing User: 9805361 . Digilib
Date Deposited: 21 Jan 2016 07:34
Last Modified: 21 Jan 2016 07:34
URI: http://digilib.unila.ac.id/id/eprint/18788

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