POLA RESISTENSI ANTIBIOTIK DAN PROFIL PLASMID ISOLAT Pseudomonas aeruginosa DI RSUD ABDUL MOELOEK PROVINSI LAMPUNG

IWAN SARIYANTO, 1327011026 (2015) POLA RESISTENSI ANTIBIOTIK DAN PROFIL PLASMID ISOLAT Pseudomonas aeruginosa DI RSUD ABDUL MOELOEK PROVINSI LAMPUNG. Masters thesis, UNIVERSITAS LAMPUNG.

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Abstrak

Pseudomonas aeruginosa merupakan bakteri patogen nosokomial. Bakteri ini mempunyai kemampuan luar biasa untuk menjadi resisten terhadap beberapa antibiotik. Resistensi bakteri terhadap antibiotik telah menjadi masalah kesehatan dunia terutama terjadinya fenomena MDR (multidrug resistant). Beberapa penelitian menunjukkan bahwa resistensi antibiotik dapat ditransfer ke bakteri lain melalui plasmid. Penelitian ini dilakukan untuk menentukan pola resistensi antibiotik dan profil plasmid isolat Pseudomonas aeruginosa dari RSUDAM Provinsi Lampung. Uji resistensi isolat dilakukan mengikuti metode difusi cakram Kirby-Bauer. Plasmid diisolasi dengan metode lisis alkali menggunakan Kit Plasmid-Miniprep, dan dilektrofosesis dengan gel agarosa 1%. Tiga puluh isolat Pseudomonas aeruginosa didapatkan dari 782 sampel pada Bulan Februari hingga Mei 2015. Distribusi sampel klinis Pseudomonas aeruginosa pada nanah 66,7%, dahak 16,7%, darah 13,3%, dan urine 3,3%. Semua isolat resisten terhadap Ampisilin. Resistensi terhadap Kloramfenikol 83,3%, Cefoperazone 60%, Gentamicin 43,3%, Siprofloxacin 36,7% dan Meropenem 6,7%. Isolat Pseudomonas aeruginosa yang mengalami MDR sebanyak 97%. Berdasarkan hasil elektroforesis menunjukkan bahwa 14 Isolat (46,8%) memberikan pita plasmid. Tiga belas isolat memiliki pita plasmid tunggal dan hanya satu isolat memiliki dua pita. Ukuran plasmid berkisar dari 1,4 kb hingga 9 kb. Analisis statistik menunjukkan adanya korelasi antara pola resistensi antibiotik dengan profil plasmid Pseudomonas aeruginosa. Kata kunci: Pseudomonas aeruginosa, resistensi antibiotik, profil plasmid ABSTRAK BAHASA INGGRIS Pseudomonas aeruginosa is a nosocomial pathogenic bacteria. These bacteria has the remarkable ability to become resistant to some antibiotics. Bacterial resistance to antibiotics has become a global health problem, especially the phenomenon of MDR (multidrug resistant). Some research suggests that antibiotic resistance can be transferred to other bacteria via plasmids. This study was undertaken to determine the antibiotic resistance pattern and plasmid’s profiles of Pseudomonas aeruginosa from RSUDAM Lampung’s province. The resistance test of the isolates was performed following the Kirby-Bauer disc diffusion method. The plasmid were isolated using alkalyne lysis method by using Plasmid-Miniprep Kit, and electrophorated on 1% agarose gel. Thirty isolates of Pseudomonas aeruginosa, obtained from 782 samples in February to May 2015. The distribution of Pseudomonas aeruginosa among the clinical samples of pus 66,7%, sputum 16,7%, blood 13,3%, and urine 3,3%. All isolates were resistant to Ampicillin. Resistance to Chloramphenicol 83.3%, Cefoperazone 60%, Gentamicin 43,3%, Ciprofloxacin 36,7%, and Meropenem 6,7%. Based on electrophoresis results showed that 14 Isolates (46.8%) giving band plasmids. Thirteen of the isolates had a single plasmid band and only one isolate possessed two bands. The sizes of the plasmids ranged from 1,4 kb to 9 kb. Statistical analysis showed a correlation between the pattern of antibiotic resistance with plasmid’s profiles of Pseudomonas aeruginosa. Keywords : Pseudomonas aeruginosa, antibiotic-resistance, plasmid profiles.

Tipe Karya Ilmiah: Tesis (Masters)
Subyek: Q Science (General) > QD Chemistry
Pengobatan > R Medicine (General)
Program Studi: Fakultas MIPA > Prodi Magister Ilmu Kimia
Depositing User: 9999154 . Digilib
Date Deposited: 21 Jan 2016 02:04
Last Modified: 21 Jan 2016 02:04
URI: http://digilib.unila.ac.id/id/eprint/18791

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