Febri Windi Asmoro, 1117011020 (2016) PENENTUAN KADAR GLUKOSAMIN DARI SUBSTRAT KULIT UDANG MENGGUNAKAN BAKTERI Actinomycetes ANL-4 SEBAGAI PENDEGRADASI DALAM VARIASI WAKTU INKUBASI. UNIVERSITAS LAMPUNG, FAKULTAS MATEMATIKA DAN ILMU PENGETAHUAN ALAM.
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Abstract
Penelitian ini dilakukan untuk mengubah kitin dari substrat kulit udang menjadi glukosamin menggunakan Actinomycetes ANL-4. Actinomycetes ANL-4 menghasilkan enzim kitinase dan enzim kitin deasetilase. Produksi glukosamin dilakukan secara fermentasi dengan sistem tertutup (Batch). Waktu fermentasi dilakukan untuk 1, 2, 3, 4, dan 5 hari. Produk berupa glukosamin diperoleh paling tinggi pada hari pertama sebesar 72%. Produk glukosamin direaksikan dengan ninhidrin dan dianalisis menggunakan spektofotometri UV-Vis. Hasil analisis menunjukkan kadar kemurnian glukosamin paling tinggi didapat pada hari pertama yaitu sebesar 0,0638 %. Kata kunci : Actinomycetes ANL-4, Fermentasi Batch, Spektrofotometer UV-Vis ABSTRACT This research was conducted to convert enzyme of chitin to glucosamine from shrimp shell by using Actinomycetes ANL-4. Actinomycetes ANL-4 produces chitinase and deasetilase enzymes. The production of glucosamine for that process was performed by fermentation with batch system. The fermentation period was carried out for 1, 2, 3, 4 and 5 days. The highest result of glucosamine production was acquired in first day, about 72%. Then, the product was mixed with ninhidrin and analyzed by using UV-Vis spectrofhotometry. The analysis’s result showed that the highest purity of glucosamine was acquired at the first day it.e. 0,0638%. Kata kunci : Actinomycetes ANL-4, Fermentasi Batch, Spektrofotometer UV-Vis
| Item Type: | Other |
|---|---|
| Subjects: | Q Science (General) |
| Divisions: | Fakultas MIPA > Prodi Kimia |
| Depositing User: | 4138631 . Digilib |
| Date Deposited: | 07 Dec 2016 06:52 |
| Last Modified: | 07 Dec 2016 06:52 |
| URI: | http://digilib.unila.ac.id/id/eprint/24598 |
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