ISOLASI DAN IDENTIFIKASI GEN RESISTENSI CIPROFLOXACIN PADA ISOLAT ESCHERICHIA COLI MULTIDRUGS RESISTANCE DARI PENDERITA INFEKSI SALURAN KEMIH DI RSUD ABDOEL MOELOEK PROVINSI LAMPUNG

BASUKI RACHMAD, 1427011001 (2017) ISOLASI DAN IDENTIFIKASI GEN RESISTENSI CIPROFLOXACIN PADA ISOLAT ESCHERICHIA COLI MULTIDRUGS RESISTANCE DARI PENDERITA INFEKSI SALURAN KEMIH DI RSUD ABDOEL MOELOEK PROVINSI LAMPUNG. Masters thesis, UNIVERSITAS LAMPUNG.

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ABSTRACT THE ISOLATION AND IDENTIFICATION OF CIPROFLOXACIN RESISTANCE GENES ON ISOLAT ESCHERICHIA COLI MULTI-DRUGS RESISTANCE OF URINARY TRACT INFECTIONS PATIENT IN REGIONAL PUBLIC HOSPITAL ABDOEL MOELOEK LAMPUNG PROVINCE By BASUKI RACHMAD The increasing of Escherichia coli resistance to fluoroquinolone antibiotics has been widely reported around the world. A total of 30 E.coli isolates from patients with UTI in RSUDAM Lampung Province, was found 18 isolates of E.coli MDR have plasmid and 73.3% were resistant to ciprofloxacin. E.coli resistance to the antibiotic fluoroquinolone (eg ciprofloxacin) is generally caused by chromosomal mutations in genes gyrA and parc, and gene plasmid. This study aims to determine the presence of ciprofloxacin resistance gene, both contained in plasmid DNA (plasmid mediated quinolone resistance) is qnr, oqxA and oqxB and the chromosomal DNA (quinolone Resistance Determining Regions) is gyrA and parC. Isolation of plasmid and chromosomal DNA of the 18 isolates was performed using each kit accordingly. The existence of genes in the isolates were detected by PCR using Thermal Cycler T100TM. Electrophoresis in 1% agarose gel of amplicons using BIO-RADTM PowerPac. Visualization of amplicons with BIO-RADTM UVITEC produce DNA gene fragments qnr at 593 bp (1 isolate), oqxA (866 bp, 2 isolates), oqxB (781 bp, 2 isolates), gyrA (191 bp, 18 isolates) and parC (264 bp, 18 isolates). From these data can be proposed that resistance to ciprofloxacin allegedly caused by the presence of ciprofloxacin resistance gene, both of which are located in the plasmid and chromosome. DNA sequencing is recommended to determine the sequence of bases on the tape produced so that the pattern of mutations in these genes are associated with MDR can be solved. Key words: ciprofloxacin resistence E.coli, qnr gene, oqxA, oqxB, gyrA, parC   ABSTRAK ISOLASI DAN IDENTIFIKASI GEN RESISTENSI CIPROFLOXACIN PADA ISOLAT ESCHERICHIA COLI MULTIDRUGS RESISTANCE DARI PENDERITA INFEKSI SALURAN KEMIH DI RSUD ABDOEL MOELOEK PROVINSI LAMPUNG Oleh BASUKI RACHMAD Peningkatan resistensi Escherichia coli terhadap antibiotik fluorokuinolon telah banyak dilaporkan di seluruh dunia. Sebanyak 30 isolat E.coli dari pasien ISK di RSUDAM Provinsi Lampung, ditemukan 18 isolat E.coli MDR mempunyai plasmid dan 73,3% resisten terhadap ciprofloxacin. Resistensi E.coli terhadap antibiotik fluorokuinolon (misal ciprofloxacin) umumnya disebabkan mutasi kromosom pada gen gyrA dan parC, dan adanya gen plasmid. Penelitian ini bertujuan untuk mengetahui keberadaan gen resistensi ciprofloxacin, baik yang terdapat pada DNA plasmid (Plasmid Mediated Quinolone Resistance) yaitu qnr, oqxA dan oqxB maupun pada DNA kromosom (Quinolone Resistance Determining Regions) yaitu gyrA dan parC. Isolasi DNA plasmid dan kromosom dilakukan terhadap 18 isolat tadi menggunakan masing-masing kit yang sesuai. Keberadaan gen di dalam isolat dideteksi secara PCR menggunakan T100TM Thermal Cycler. Elektroforesis di dalam gel agarosa 1% dari amplikon menggunakan BIO-RADTM PowerPac. Visualisasi amplikon dengan BIO-RADTM UVITEC menghasilkan pita DNA fragmen gen qnr pada 593 bp (1 isolat), oqxA (866 bp, 2 isolat), oqxB (781 bp, 2 isolat), gyrA (191 bp, 18 isolat) dan parC (264 bp, 18 isolat). Dari data tersebut dapat diusulkan bahwa resistensi terhadap ciprofloxacin diduga kuat disebabkan oleh adanya gen resistensi ciprofloxacin, baik yang berlokasi di plasmid maupun kromosom. Sekuensing DNA disarankan untuk menentukan urutan basa-basa pada pita yang dihasilkan sehingga pola mutasi pada gen-gen tersebut yang berkaitan dengan MDR dapat dipecahkan. Kata Kunci : E.coli resisten ciprofloxacin, gen qnr, oqxA, oqxB, gyrA, parC

Tipe Karya Ilmiah: Tesis (Masters)
Subyek: Q Science (General)
Q Science (General) > QD Chemistry
Program Studi: Fakultas MIPA > Prodi Magister Ilmu Kimia
Depositing User: 9197702 . Digilib
Date Deposited: 06 Feb 2017 07:49
Last Modified: 06 Feb 2017 07:49
URI: http://digilib.unila.ac.id/id/eprint/25479

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