ANALISIS KERAGAMAN GENETIK TIGA VARIETAS UDANG GALAH, Macrobrachium rosenbergii (de Man, 1879) MENGGUNAKAN PENANDA DNA MITOKONDRIA

FETRILISA SILITONGA, 1414111028 (2018) ANALISIS KERAGAMAN GENETIK TIGA VARIETAS UDANG GALAH, Macrobrachium rosenbergii (de Man, 1879) MENGGUNAKAN PENANDA DNA MITOKONDRIA. FAKULTAS PERTANIAN, UNIVERSITAS LAMPUNG.

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Abstrak

Analisis DNA mitokondria sering digunakan untuk mempelajari keragaman genetik populasi dan hubungan filogenetik melalui pola pewarisan maternal. Penelitian ini bertujuan untuk mengidentifikasi keragaman genetik tiga varietas udang galah (populasi aceh, populasi solo, dan strain siratu) mengunakan metode RFLP. Genom mtDNA diekstrak menggunakan metode phenol-chloroform dan selanjutnya diamplifikasi dengan teknik polymerase chain reaction (PCR) menggunakan primer COI (forward 5’ -ACG CAA CGG TGG CTT TTC-3’; reverse 5’-TAG TTA GCT GTT AGG GGG AT-3’) dan primer 16S rDNA (forward 5’-GGT AAT TTG ACC GTG CGA AG -3’; reverse 5’-ACC AGC TAA ACG GCT ACA CCT -3’). Produk PCR COI sepanjang 1.516 pasang basa (bp) direstriksi menggunakan 5 enzim (Ava II, Msp I, Rsa I, Hae III, dan Taq I). Adapun produk PCR primer 16S rDNA sepanjang 444 pasang basa (bp) direstriksi menggunakan enzim Mbo I, Msp I, Sau3A II, Hae III, dan Taq I). Hasil penelitian menunjukkan pada region COI terdapat 22 haplotipe, sedangkan pada region 16S rDNA menunjukkan 2 haplotipe. Berdasarkan ada tidaknya situs restiksi pada masing-masing mtDNA, diketahui bahwa nilai keragaman genetik pada mtDNA COI populasi aceh 0,836, strain siratu 0,829 dan populasi solo 0,517. Adapun pada mtDNA 16S rDNA populasi aceh 0,488, strain siratu 0,066 dan populasi solo 0.000. Jarak genetik menggunakan region COI dan 16S rDNA menunjukkan bahwa populasi aceh-solo yang tertinggi (jauh) dengan nilai berturut-turut 0,0800 dan 0,6170. Kata kunci : keragaman genetik, Macrobrachium rosenbergii, COI, 16S rDNA iii ABSTRACT Mitochondrial DNA analysis is often used to study genetic diversity and phylogenetic relationships of the population through maternal inheritance pattern. This study aimed to identify the genetic diversity of three varieties of prawns (the population of aceh, the population of solo, and the strain siratu) using a method of RFLP. Genomic mtDNA was extracted by the method of phenol-chloroform, then the amplified through of polymerase chain reaction (PCR) method using COI primer (forward 5’ -ACG CAA CGG TGG CTT TTC-3’; reverse 5’-TAG TTA GCT GTT AGG GGG AT-3’) and 16S rDNA primer (forward 5’-GGT AAT TTG ACC GTG CGA AG -3’; reverse 5’-ACC AGC TAA ACG GCT ACA CCT -3’). PCR products of COI along the 1,516 base pairs (bp) restriction by using 5 enzymes (Ava II, Msp I, Rsa I, Hae III, and Taq I) and 16S rDNA along the 444 base pairs (bp) using the enzyme Mbo I, Msp I, Sau3A I, Hae III, and Taq I). This research showed that the COI region had 22 haplotypes and 16S rDNA region had 2 haplotypes. Based on the presence of restrictions site in each mtDNA COI showed the genetic diversity of aceh, siratu, and solo population were 0.836, 0.829, and 0.517 respectively. As for the mtDNA 16S rDNA known that genetic diversity of aceh, siratu, and solo were 0.488, 0.066, and 0.000. The genetic distance determined by using COI and 16S rDNA regions showed that the genetic diversity between aceh and solo populations was furthest, i.e 0.0800 (COI) and 0.6170 (16S rDNA). Keywords: genetic diversity, Macrobrachium rosenbergii, COI, 16S rDNA

Tipe Karya Ilmiah: Skripsi
Subyek: Pertanian > Budidaya . Perikanan . Angling
Program Studi: Fakultas Pertanian > Prodi Budidaya Perairan
Depositing User: 201888520 . Digilib
Date Deposited: 05 Sep 2018 07:12
Last Modified: 05 Sep 2018 07:12
URI: http://digilib.unila.ac.id/id/eprint/33110

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