Chintia Yolanda, 1017011005 (2014) PENETAPAN WAKTU INKUBASI OPTIMUM DEGRADASI KITIN SECARA ENZIMATIK OLEH Mucor miehei DENGAN METODE ULTRAVIOLET-VISIBLE SPECTROPHOTOMETRY. Fakultas MIPA, Universitas Lampung.
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Abstract
Penelitian ini dilakukan untuk mempelajari potensi enzim kitinase dan kitindeasetilase yang dihasilkan Mucor miehei dalam mendegradasi kitin menjadi glukosamin setiap hari selama tujuh hari proses fermentasi. Kadar glukosamin ditentukan berdasarkan metode spektrofotometri UV-Vis dan analisis kemurnian glukosamin dengan metode HPLC-ELSD. Hasil penelitian menunjukkan fermentasi kitin dengan Mucor miehei menghasilkan rendemen glukosamin sejak hari pertama fermentasi dan mencapai rendemen maksimum sebesar 90 % berdasarkan penimbangan dan 88 % berdasarkan pengukuran spektrofotometri UV-Vis pada hari keempat dengan kemurnian sebesar 97,342 %. Produksi glukosamin dikonfirmasi dengan analisis HPLC-ELSD. Hasil analisis menunjukkan adanya puncak pada rentang waktu retensi 2-3 menit yang sama seperti pada kromatogram glukosamin standar produksi WAKO, Jepang. Kata kunci : Kitin, Glukosamin,Mucor miehei, Spektrofotometri UV-Vis, HPLC-ELSD Abstrak Bahasa Inggris This research was conducted to study the potential of chitinase and chitin deacetylase produced by Mucor miehei to degrade chitin into glucosamine every day for seven days fermentation process. Glucosamine was determined using the method of UV-Vis spectrophotometry and the purity of the glucosamine was analysed using HPLC-ELSD method. The results show chitin fermentation with Mucor miehei was produced glucosamine from the first day of fermentation and reached the maximum yield of 90 % by mass and 88 % according to UV-Vis spectrophotometry measurement on the fourth day with the purity of 97,342 %. The production of glucosamine was confirmed by HPLC-ELSD analysis. The results show the presence of peak at retention time of 2-3 min, which is similar to the chromatogram of standard glucosamine produced by WAKO, Japan. Keywords: Chitin, Glucosamine, Mucor miehei, UV-Vis Spectrophotometry, HPLC-ELSD
| Item Type: | Other |
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| Subjects: | A General Works = Karya Karya Umum |
| Divisions: | Fakultas MIPA > Prodi Kimia |
| Depositing User: | 945129 . Digilib |
| Date Deposited: | 13 Oct 2014 03:27 |
| Last Modified: | 13 Oct 2014 03:27 |
| URI: | http://digilib.unila.ac.id/id/eprint/4039 |
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