Jelita Noviantina, 1014111041 (2014) Uji Patogenisitas Bakteri Campylobacter sp. TI6, Listeria sp. TI1, dan Nitrosococcus sp. TII5 pada Pemeliharaan Udang Vannamei (Litopenaeus vannamei) serta Kemampuannya Mendegradasi TAN (Total Ammonia Nitrogen). FAKULTAS PERTANIAN, UNILA.
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Abstract
Tiga isolat bakteri indigenous dari tambak udang di Lampung Selatan yaitu Campylobacter sp. TI6, Listeria sp. TI1, dan Nitrosococcus sp. TII5 terbukti mampu menurunkan nilai Total Ammonia Nitrogen (TAN) secara in vitro. Penelitian ini dilakukan untuk mengetahui tingkat patogenisitas bakteri Campylobacter sp. TI6, Listeria sp. TI1, dan Nitrosococcus sp. TII5 terhadap larva udang vannamei dan efektivitas bakteri tersebut dalam mendegradasi TAN pada skala laboratorium. Uji patogenisitas dilakukan dengan metode LD50, masing-masing bakteri disediakan dalam kepadatan 103, 104, 105, dan 106 CFU/ml. Hasil LD50 menunjukkan bahwa ketiga bakteri tersebut tidak bersifat patogen karena tidak ada konsentrasi bakteri yang mematikan larva udang lebih dari 50%. Kepadatan bakteri yang digunakan untuk uji kemampuan bakteri dalam mendegradasi TAN adalah 106 CFU/ml. Uji kemampuan bakteri dalam mendegradasi TAN dilakukan secara in vivo dengan 2 perlakuan yaitu pemeliharaan udang vannamei yang diberi bakteri bioremediasi dan limbah organik serta pemeliharaan udang vannamei yang tidak diberi bakteri bioremediasi, tetapi tetap diberi limbah organik. Hasil penelitian menunjukkan bahwa bakteri Campylobacter sp. TI6 dapat menurunkan nilai TAN sebesar 0,08 mg/L, Listeria sp. TI1 sebesar 0,03 mg/L dan Nitrosococcus sp. TII5 sebesar 0,04 mg/L. Kata kunci : Campylobacter sp. TI6, Listeria sp. TI1, Nitrosococcus sp. TII5, Total Ammonia Nitrogen (TAN), Litopenaeus vannamei Three indigenous bacterial isolates from shrimp ponds in South Lampung namely Campylobacter sp. TI6, Listeria sp. TI1, and Nitrosococcus sp. TII5 were proven to reduce the value of Total Ammonia Nitrogen (TAN) in vitro. This study was conducted to determine level of pathogenicity from Campylobacter sp. TI6, Listeria sp. TI1, and Nitrosococcus sp. TII5 toward the vannamei shrimp larvae and effectiveness of these bacteria to degrade TAN at the laboratory scale. Pathogenicity test was conducted using the LD50, respectively provided in the density of bacteria 103, 104, 105, and 106 CFU/ ml. The results showed that those bacteria were not pathogenic because there was no concentration of bacteria that able killed the shrimp larvae more than 50%. Density of bacteria that was used in the test of degrading ability of TAN was 10¬6 CFU/ ml. The ability test of bacteria to degrade TAN was performed in vivo with 2 treatments i.e. rearing of vannamei shrimp with using bacterial bioremediation and organic and the rearing of vannamei shrimp without bacterial bioremediation, but still considering the organic waste. The results showed that the bacteria Campylobacter sp. TI6 could reduce the TAN value of 0.08 mg/ L, Listeria sp. TI1 of 0.03 mg/ L and Nitrosococcus sp. TII5 of 0.04 mg/ L. Keywords : Campylobacter sp. TI6, Listeria sp. TI1, Nitrosococcus sp. TII5, Total Ammonia Nitrogen (TAN), Litopenaeus vannamei
| Item Type: | Other |
|---|---|
| Subjects: | A General Works = Karya Karya Umum |
| Divisions: | Fakultas Pertanian > Prodi Budidaya Perairan |
| Depositing User: | 7241689 . Digilib |
| Date Deposited: | 27 Oct 2014 07:32 |
| Last Modified: | 27 Oct 2014 07:32 |
| URI: | http://digilib.unila.ac.id/id/eprint/4797 |
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