PENINGKATAN STABILITAS ENZIM SELULASE DARI Bacillus subtilis ITBCCB148 DENGAN MODIFIKASI KIMIA MENGGUNAKAN ASAM GLIOKSILAT

Rina Rachmawati Sutisna, 1017011069 (2014) PENINGKATAN STABILITAS ENZIM SELULASE DARI Bacillus subtilis ITBCCB148 DENGAN MODIFIKASI KIMIA MENGGUNAKAN ASAM GLIOKSILAT. Fakultas Mipa, UNIVERSITAS LAMPUNG.

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Abstrak

ABSTRAK Penelitian ini bertujuan untuk meningkatkan stabilitas enzim selulase dari isolat bakteri lokal Bacillus subtilis ITBCCB148 dengan modifikasi menggunakan asam glioksilat. Untuk mencapai tujuan tersebut, dilakukan produksi, isolasi, pemurnian, modifikasi glioksilat dan karakterisasi enzim selulase hasil pemurnian sebelum dan sesudah modifikasi. Hasil penelitian menunjukan enzim hasil pemurnian memiliki aktivitas unit 6,9753 U/mL, lebih murni dibandingkan dengan ekstrak kasar enzim yang mempunyai aktivitas unit 0,5963 U/mL. Enzim hasil pemurnian dan hasil modifikasi mempunyai pH optimum yang sama yaitu 6,0 dan suhu optimum yang sama yaitu 60oC. Enzim hasil modifikasi asam glioksilat memiliki derajat modifikasi 70,54; 78,68; 68,43%. Stabilias termal enzim hasil modifikasi dengan derajat modifikasi 70,54; 78,68; 68,43% berturut-turut: t1/2 = 22,35 menit, ki = 0,031 menit-1, ΔGi = 102,8253 kJ mol-1; t1/2 = 21,00 menit, ki = 0,033 menit-1, ΔGi = 102,6522 kJ mol-1; : t1/2 = 18,72 menit, ki = 0,037 menit-1, ΔGi = 102,3354 kJ mol-1, dan data stabilitas termal enzim hasil pemurnian: t1/2 = 10,50 menit, ki = 0,066 menit-1, ΔGi = 100,7330 kJ mol-1. Modifikasi kimia terhadap enzim selulase dapat meningkatkan kestabilan enzim terhadap pH dan suhu serta meningkatkan stabilitas termal enzim. Kata kunci : Bacillus subtilis ITBCCB148, selulase, modifikasi kimia, asam glioksilat ABSTRACT The research aims to increase the thermal stability of enzyme cellulase obtained from locale bacteria isolate Bacillus subtilis ITBCCB148 using modification process with glyoxylic acid. To approach this aims, the production, isolation, purification, modificatin and characterization purified cellulase enzyme were done. The results showed that the native enzyme has a unit activity 6,9753 U/mL, purer than crude extract enzyme which has unit activity 0,5963 U/mL. The native and the modified enzyme have similar pH of 6,0 and optimum temperature of 60oC. The modified enzymes with glyoxylic acid produced modified enzyme with modification degree of 70,54; 78,68; 68,43%. The enzyme thermal stability of the modified enzyme with modification degree of 70,54; 78,68; 68,43% at 60oC were shown with the following data : t1/2 = 22,35 min, ki = 0,031 min-1, ΔGi = 102,8253 kJ mole-1; t1/2 = 21,00 min, ki = 0,033 min-1, ΔGi = 102,6522 kJ mole-1; : t1/2 = 18,72 min, ki = 0,037 min-1, ΔGi = 102,3354 kJ mole-1; respectively, whereas the thermal stability of the purified enzyme has data: t1/2 = 10,50 min, ki = 0,066 min-1, ΔGi = 100,7330 kJ mole-1. The chemical modification on the purified cellulase enzyme has been able to increase the stability to optimum pH and optimum temperature and the thermal stability. Keywords : Bacillus subtilis ITBCCB148, cellulase, chemical modification, glyoxylic acid

Tipe Karya Ilmiah: Skripsi
Subyek: A General Works = Karya Karya Umum
Program Studi: Fakultas MIPA > Prodi Kimia
Depositing User: A.Md Cahya Anima Putra .
Date Deposited: 12 Dec 2014 02:37
Last Modified: 16 Apr 2015 06:48
URI: http://digilib.unila.ac.id/id/eprint/5572

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