IDENTIFIKASI SINGLE NUCLEOTIDE POLYMORPHISM (SNP) GEN THROMBOSPONDIN-RELATED ANONYMOUS PROTEIN (TRAP) PADA PENDERITA MALARIA falciparum DI WILAYAH KERJA PUSKESMAS HANURA, KABUPATEN PESAWARAN, PROVINSI LAMPUNG

MUHAMMAD IRFAN ADI SHULHAN, 1518011004 (2019) IDENTIFIKASI SINGLE NUCLEOTIDE POLYMORPHISM (SNP) GEN THROMBOSPONDIN-RELATED ANONYMOUS PROTEIN (TRAP) PADA PENDERITA MALARIA falciparum DI WILAYAH KERJA PUSKESMAS HANURA, KABUPATEN PESAWARAN, PROVINSI LAMPUNG. FAKULTAS ILMU KEDOKTERAN, UNIVERSITAS LAMPUNG.

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Abstrak (Berisi Bastraknya saja, Judul dan Nama Tidak Boleh di Masukan)

Background: Malaria is a disease caused by the Plasmodium parasite in erythrocytes as evidenced by positive microscopic examination, there are antigens, and the discovery of Deoxyribo Nucleid Acid / Ribonucleid Acid (DNA / RNA) Plasmodium parasite on Polymerase Chain Reaction (PCR). Plasmodium falciparum is one of the most deadly species of malaria, and there are many genes that support this parasite to infect, so there are so many genetic variations produced by one gene. Method: This type of research uses a survey research design with descriptive method. The study sample was obtained from 18 Stored Archived Biological Materials (ABM). This research was conducted using the PCR method which was analyzed by gel electrophoresis and continued to the sequencing method to detect genetic variations of the Plasmodium falciparum Thrombospondin-related anonymous protein (PfTRAP) gene. Result: There were 18 samples that had been carried out nested PCR, then obtained variations in the length of the band in each sample Conclusion: There are genetic variation in the Thrombospondin-related anonymous protein (TRAP) gene. Keyword: Malaria, Plasmodium Falciparum Thrombospondin-related anonymous protein (PfTRAP), Polymerase Chain Reaction (PCR), Sequencing. Latar Belakang: Malaria merupakan penyakit yang disebabkan oleh parasit Plasmodium di dalam eritrosit dibuktikan dengan pemeriksaan mikroskopis yang positif, terdapat antigen, serta ditemukannya Deoxyribo Nucleid Acid/ Ribonucleid Acid (DNA/RNA) parasit Plasmodium pada pemeriksaan Polymerase Chain Reaction (PCR). Plasmodium falciparum adalah salah satu spesies yang paling banyak menyebabkan kasus malaria dan paling mematikan, serta terdapat banyak gen yang mendukung parasit ini untuk menginfeksi, sehingga banyak sekali variasi genetik yang dihasilkan oleh satu gen. Metode: Jenis penelitian ini menggunakan rancangan penelitian survey dan bersifat deskriptif. Sampel penelitian diperoleh dari Bahan Biologi Tersimpan (BBT) sebanyak 18 sampel. Penelitian ini dilakukan dengan menggunakan metode PCR yang dianalisis dengan gel elektroforesis serta dilanjutkan ke metode sekuensing untuk mendeteksi variasi genetik gen Plasmodium falciparum Thrombospondin-related anonymous protein (PfTRAP). Hasil: Terdapat 18 sampel yang telah dilakukan nested PCR, kemudian diperoleh variasi panjang pita pada setiap sampel. Kesimpulan: Terdapat variasi genetik gen Thrombospondin-related anonymous protein (TRAP). Kata Kunci: Malaria, Plasmodium Falciparum Thrombospondin-related anonymous protein (PfTRAP), Polymerase Chain Reaction (PCR), Sekuensing.

Jenis Karya Akhir:	Skripsi
Subyek:
Program Studi:	Fakultas Kedokteran > Prodi Pendidikan Dokter
Pengguna Deposit:	UPT . Teti Novianti
Date Deposited:	25 Mar 2022 07:52
Terakhir diubah:	25 Mar 2022 07:52
URI:	http://digilib.unila.ac.id/id/eprint/55978

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