WULANDARI , AGUSTIN (2023) IDENTIFIKASI DAN ISOLASI SENYAWA BIOAKTIF ANTIINFLAMASI DARI KULIT BATANG TUMBUHAN TURI PUTIH (Sesbania grandiflora (L.) Poir) MENGGUNAKAN METABOLOMIK BERBASIS LC-MS/MS. FAKULTAS MATEMATIKA DAN ILMU PENGETAHUAN ALAM, UNIVERSITAS LAMPUNG.
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Abstrak (Berisi Bastraknya saja, Judul dan Nama Tidak Boleh di Masukan)
Turi putih (S. grandiflora) merupakan salah satu tumbuhan asli Indonesia yang biasa dimanfaatkan sebagai antiinflamasi. Tujuan penelitian ini mendapatkan senyawa bioaktif antiinflamasi dari hasil isolasi menggunakan metabolomik berbasis LC-MS/MS. Senyawa hasil isolasi didapatkan melalui ekstraksi dengan variasi konsentrasi pelarut MeOH 100%, EtOAc 100% dan MeOH:EtOAc 50% dengan uji antiinflamasi menggunakan metode penghambatan denaturasi protein. Aktivitas antiinflamasi yang paling baik diperoleh dari ekstrak EtOAc 100% dengan rata-rata nilai IC50 sebesar 117,54 g/mL. Dari hasil analisis LC-MS/MS didapatkan 106 senyawa known dan 394 senyawa unknown. Ekstrak sampel kulit batang tumbuhan turi putih dikelompokan berdasarkan variasi pelarut pengekstrak melalui analisis partial component analysis (PCA) dengan total primary component (PC) 83% saat menggunakan data luas area dan 60% saat menggunakan data intensitas. Data LC-MS/MS yang berupa luas area dan intensitas dikorelasikan dengan aktivitas antiinflamasi menggunakan orthogonal partial least squaresdiscriminant analysis. Hasil analisis OPLS-DA menunjukkan bahwa senyawa 36 (Sesbagrandiflorain C), senyawa 44 (Sesbagrandiflorain B) dan senyawa 84 (2- metil-5-{2-[2-(4-metil-3,6-dioksosikloheksa-1,4-dienil)etoksi]etil}benzo-1,4 quinon) merupakan metabolit penciri known yang diduga berkontribusi major (utama) pada aktivitas antiinflamasi ekstrak kulit batang tumbuhan turi putih. Senyawa murni berhasil diisolasi dari ekstrak etil asetat 100% kulit batang tumbuhan turi putih melalui kromatografi cair vakum (KCV) dan kromatografi kolom gravitasi (KKG). Isolat yang beraktivitas antiinflamasi diperoleh berupa kristal kuning sebanyak 7,2 mg dan 5 mg diduga sebagai senyawa sesbagrandiflorain C dan sesbagrandiflorain B berdasarkan hasil analisis dari LCMS/ MS serta OPLS-DA. Kata Kunci: S. grandiflora, metabolomik, antiinflamasi, LC-MS/MS, PCA, OPLS-DA White turi (S. grandiflora) is one native Indonesian plant which is commonly used as an anti-inflammatory. The aim of this study was to obtain anti-inflammatory bioactive compounds from the results of isolation based on LC-MS/MS-based metabolomics. The isolated compounds were obtained by extraction with varying concentrations of 100% MeOH, 100% EtOAc and 50% MeOH:EtOAc with antiinflammatory tests using the protein denaturation inhibition method. The best antiinflammatory activity was obtained from 100% EtOAc extract with an average IC50 value of 117.54 g/mL. From the results of the LC-MS/MS analysis, 106 known compounds and 394 unknown compounds were obtained. The results of the LCMS/ MS analysis, 106 known compounds and 394 unknown compounds were obtained. Extract samples of turi putih stem bark were grouped based on variations in extracting solvents through partial component analysis (PCA) with a total primary component (PC) of 83% when using area data and 60% when using intensity data. LC-MS/MS data in the form of area and intensity were correlated with anti-inflammatory activity using orthogonal partial least squares-discriminant analysis. The results of the OPLS-DA analysis showed that compound 36 (sesbagrandiflorain C), compound 44 (sesbagrandiflorain B) and compound 84 (2- methyl-5-{2-[2-(4-methyl-3,6-dioxocyclohexa-1,4)dienyl)ethoxy]ethyl}benzo- 1,4-quinone) is a known characteristic metabolite which is thought to contribute majorly to the anti-inflammatory activity of turi putih stem bark extract. The pure compound was isolated from 100% ethyl acetate extract of the bark of the turi putih plant by means of vacuum liquid chromatography (KCV) and gravity column chromatography (KKG). Isolates with anti-inflammatory activity were obtained in the form of yellow crystals of 7.2 mg and 5 mg suspected as sesbagrandiflorain C and sesbagrandiflorain B based on the analysis results from LC-MS/MS and OPLSDA. Keywords: S. grandiflora, metabolomic, anti-inflammatory, LC-MS/MS, PCA, OPLS-DA
| Jenis Karya Akhir: | Skripsi |
|---|---|
| Subyek: | 500 ilmu pengetahuan alam dan matematika 500 ilmu pengetahuan alam dan matematika > 540 Kimia dan ilmu yang berhubungan |
| Program Studi: | FAKULTAS MIPA > Prodi Kimia |
| Pengguna Deposit: | 2301165884 . Digilib |
| Date Deposited: | 18 Apr 2023 04:56 |
| Terakhir diubah: | 18 Apr 2023 04:56 |
| URI: | http://digilib.unila.ac.id/id/eprint/71085 |
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