AMPLIFIKASI GEN FORMIN BINDING PROTEIN 1-LIKE (FNBP1L) SEBAGAI TAHAP PENDAHULUAN IDENTIFIKASI MUTASI GEN YANG BERHUBUNGAN DENGAN TINGKAT INTELLIGENCE QUOTIENT (IQ)

Selvia Farahdina, 1118011124 (2015) AMPLIFIKASI GEN FORMIN BINDING PROTEIN 1-LIKE (FNBP1L) SEBAGAI TAHAP PENDAHULUAN IDENTIFIKASI MUTASI GEN YANG BERHUBUNGAN DENGAN TINGKAT INTELLIGENCE QUOTIENT (IQ). FAKULTA KEDOKTERAN, UNIVERSITAS LAMPUNG.

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Abstrak

Salah satu faktor yang mempengaruhi kecerdasan adalah genetik. Temuan menunjukkan bahwa gen yang dikenal sebagai Formin Binding Protein 1-Like (FNBP1L) secara signifikan berkaitan dengan kecerdasan anak. Penelitian ini bertujuan untuk melakukan amplifikasi Gen FNBP1L sebagai tahap pendahuluan identifikasi mutasi gen yang berhubungan dengan tingkat IQ. Penelitian ini dilaksanakan di SMA Al-Kautsar Bandar Lampung, Laboratorium Biologi Molekuler dan Biokimia Fakultas Kedokteran UNILA, dan Laboratorium Bioteknologi Fakultas Pertanian UNILA, pada bulan November 2014 – Maret 2015. Jenis penelitian ini berupa eksperimental dengan jumlah sampel dalam penelitian ini berjumlah 69 sampel darah manusia dengan teknik pengambilan sampel dengan metode simple random sampling. Selanjutnya dilakukan isolasi DNA total dan amplifikasi Gen FNBP1L yang dilakukan dengan menggunakan PCR Konvensional TC5000. Hasil amplifikasi PCR dibaca menggunakan elektroforesis gel agarose dan kemudian dilihat menggunakan sinar ultraviolet (UV). Hasil penelitian menunjukkan bahwa Gen FNBP1L dengan panjang 287 bps dapat diamplifikasi sebagai tahap pendahuluan identifikasi mutasi gen dengan suhu optimal untuk melakukan amplifikasi PCR gen adalah 55°C. Kata kunci: amplifikasi, Gen FNBP1L, kecerdasan, PCR Konvensional. One of the factors that influence intelligence is genetic. The research discovery shows that the gene known as FNBP1L is significantly associated with children's intelligence. The research's aim is to perform Gene FNBP1L amplification as a preliminary stage to identify the gene's mutation associated with IQ level. The research was conducted at SMA Al-Kautsar Bandar Lampung, Biology Molecular and Biochemistry Laboratory of Medical Faculty, and Biotechnology Laboratory of Agriculture Faculty at University of Lampung, in November 2014 - March 2015. The method of the research was based on an experiment with 69 human blood samples and a sampling method namely simple random sampling. Furthermore, total DNA isolation and amplification of FNBP1L gene were performed using the Conventional PCR TC5000. The results of PCR amplification was carried out by using electrophoresis agarose gel and then viewed using ultraviolet light (UV). The results showed that the Gene FNBP1L with a length of 287 bps could be amplified as a preliminary stage to identify the gene's mutation with the optimum temperature of 55°C to perform PCR amplification of the gene. Key words: amplification, Conventional PCR, Gene FNBP1L, intelligence.

Tipe Karya Ilmiah: Skripsi
Subyek: Pengobatan > R Medicine (General)
Pengobatan > RC Internal medicine > RC0321 Neuroscience. Biological psychiatry. Neuropsychiatry
Program Studi: Fakultas Kedokteran > Prodi Pendidikan Dokter
Depositing User: 7428794 . Digilib
Date Deposited: 10 Apr 2015 03:15
Last Modified: 10 Apr 2015 03:15
URI: http://digilib.unila.ac.id/id/eprint/8180

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