GAMBARAN RESISTANSI Vibrio parahaemolyticus (FUJINO et al., 1951) TERHADAP TIGA JENIS ANTIBIOTIK PADA BUDI DAYA UDANG VANNAMEI Litopenaeus vannamei (BOONE, 1931) DI PROVINSI BANTEN

Vidya , Ayu Seftiana (2024) GAMBARAN RESISTANSI Vibrio parahaemolyticus (FUJINO et al., 1951) TERHADAP TIGA JENIS ANTIBIOTIK PADA BUDI DAYA UDANG VANNAMEI Litopenaeus vannamei (BOONE, 1931) DI PROVINSI BANTEN. FAKULTAS PERTANIAN, UNIVERSITAS LAMPUNG .

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Abstrak (Berisi Bastraknya saja, Judul dan Nama Tidak Boleh di Masukan)

Paparan limbah antibiotik di perairan laut menimbulkan mutasi gen yang menye- babkan bakteri mengalami resistansi terhadap antibiotik. Penggunaan air pada bu- di daya udang tanpa treatment memungkinkan bakteri yang resistan terhadap anti- biotik dapat masuk ke lingkungan budi daya berpotensi menyebabkan penyakit pada udang dan sulit ditanggulangi. Penelitian ini bertujuan untuk mempelajari re- sistansi bakteri V. parahaemolyticus terhadap tiga jenis antibiotik pada tambak dan hatchery di Provinsi Banten. Metode yang digunakan adalah eksploratif. Seba- nyak 120 sampel udang vannamei diambil dari tambak dan hatchery udang van- namei (Litopenaeus vannamei) di Provinsi Banten. Isolat terduga V. parahaemoly- ticus diidentifikasi dengan uji biokimia, kemudian dikonfirmasi menggunakan PCR konvensional. Sebanyak 21 isolat V. parahaemolyticus diuji menggunakan difusi cakram dengan tiga antibiotik. Hasil uji menunjukkan persentase sensitivi- tas V. parahaemolyticus sebesar 100% sensitif terhadap tetrasiklin, 100% sensitif terhadap oksitetrasiklin, dan 19,05% intermediat resistan terhadap enrofloksasin. Nilai minimum inhibitory concentration (MIC) isolat intermediat resistan adalah 2 μg/mL. V. parahaemolyticus dengan interpretasi intermediat resistan berpotensi untuk menyebarkan gen resistan ke bakteri lain. Kata kunci: Antibiotik, enrofloksasin, oksitetrasiklin, resistansi, tetrasiklin, Vibrio parahaemolyticus. Exposure to antibiotic waste in marine waters causes gene mutations that caused bacteria to develop resistance to antibiotics. The use of water without treatment in shrimp culture allows bacteria that are resistant to antibiotics to enter the cultiva- tion environment, potentially causing disease in shrimp and being difficult to con- trol. This study aimed to determine the resistance of V. parahaemolyticus bacteria to three types of antibiotics in ponds and hatcheries in Banten Province. The me- thod used was exploratory. A total of 120 samples of vannamei shrimp were taken from ponds and hatcheries of vannamei shrimp (Litopenaeus vannamei) in Banten Province. Suspected isolates of V. parahaemolyticus were identified using bioche- mical tests, then confirmed using conventional PCR. A total of 21 V. parahaemo- lyticus isolates were tested using disk diffusion with three antibiotics. The test re- sults showed that the sensitivity percentage of V. parahaemolyticus was 100% sensitive to tetracycline, 100% sensitive to oxytetracycline, and 19.05% interme- diate resistant to enrofloxacin. The minimum inhibitory concentration (MIC) for resistant intermediate isolates was 2 μg/mL. V. parahaemolyticus with the inter- pretation of intermediate resistance had the potential to spread resistance genes to other bacteria. Keywords: Antibiotics, enrofloxacin, oxytetracycline, resistance, tetracycline, Vibrio parahaemolyticus.

Jenis Karya Akhir:	Skripsi
Subyek:	600 Teknologi (ilmu terapan) > 630 Pertanian dan teknologi yang berkaitan
Program Studi:	Fakultas Pertanian dan Pascasarjana > Prodi Budidaya Perairan
Pengguna Deposit:	. . Yulianti
Date Deposited:	19 Feb 2025 03:04
Terakhir diubah:	19 Feb 2025 03:04
URI:	http://digilib.unila.ac.id/id/eprint/84457

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