PENGARUH PENAMBAHAN L-CARNITINE DENGAN DOSIS YANG BERBEDA DALAM BAHAN PENGENCER TRIS KUNING TELUR TERHADAP KUALITAS SEMEN CAIR DOMBA EKOR TIPIS

Made , Saturdayana (2024) PENGARUH PENAMBAHAN L-CARNITINE DENGAN DOSIS YANG BERBEDA DALAM BAHAN PENGENCER TRIS KUNING TELUR TERHADAP KUALITAS SEMEN CAIR DOMBA EKOR TIPIS. FAKULTAS PERTANIAN, UNIVERSITAS LAMPUNG.

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Abstrak (Berisi Bastraknya saja, Judul dan Nama Tidak Boleh di Masukan)

Penelitian ini bertujuan untuk mengetahui pengaruh penambahan L-Carnitine terhadap motilitas, viabilitas, dan abnormalitas semen cair Domba Ekor Tipis dalam pengencer tris kuning telur. Penelitian ini dilaksanakan pada tanggal 7-8 Desember 2023 di Jurusan Peternakan, Universitas Lampung. Penelitian ini dilakukan menggunakan Rancangan Acak Lengkap dengan 4 perlakuan dan 4 ulangan. Perlakuannya adalah P0: kontrol, P1: penambahan L-Carnitine sebanyak 0,6 mg/100 ml pengencer, P2: penambahan L-Carnitine sebanyak 1,2 mg/100 ml pengencer dan P3: penambahan L-Carnitine sebanyak 2,4 mg/100 ml pengencer. Data motilitas, viabilitas, dan abnormalitas dianalisis ragam dengan taraf 5% kemudian motilitas dan viabilitas diuji lanjut polinomial ortogonal. Hasil penelitian ini menunjukkan bahwa penambahan L-Carnitine pada pengencer tris kuning telur tidak berpengaruh nyata terhadap motilitas, viabilitas, dan abnormalitas spermatozoa. Hasil polinomial ortogonal motilitas pascapengenceran menunjukkan persamaan Y = 64,752 – 2,2519 X – 0,8996 X2, X (dosis optimal) sebesar 1,25 mg dan Y (persentase tertinggi) sebesar 62,35%. Viabilitas pascapengenceran menunjukkan persamaan Y = 63,056 + 0,0818 X - 0,6752 X2, X (dosis optimal) sebesar 0,6mg dan Y (persentase tertinggi) sebesar 63,3%. Motilitas penyimpanan 3 jam menunjukkan persamaan Y = 15,623 + 4,4394 X – 2,399 X2, X (dosis optimal) sebesar 0,92mg dan Y (persentase tertinggi) sebesar 17,68%. Viabilitas 3 jam penyimpanan menunjukkan persamaan Y = 59,479 – 4,8043 X + 1,6989 X2, X (dosis optimal) sebesar 1,41mg dan Y (persentase tertinggi) sebesar 56,1%. Hasil penelitian ini dapat disimpulkan bahwa penambahan L-Carnitine yang optimal untuk motilitas pascapengenceran: 1,25mg/100ml, viabilitas pascapengenceran: 0,6mg/100ml, motilitas setelah 3 jam penyimpanan: 0,92mg/100ml, dan viabilitas setelah 3 jam penyimpanan: 1,41mg/100ml. Kata kunci : Domba Ekor Tipis, L-Carnitine, Semen Cair, Spermatozoa, Tris kuning telur ABSTRACT THE EFFECT OF L-CARNITINE WITH DIFFERENT DOSES IN TRIS EGG YOLK DILUENT ON THE QUALITY OF THIN-TAILED SHEEP LIQUID SEMEN By Made Saturdayana This study aims to determine the effect of the addition of L-Carnitine on the motility, viability, and abnormality of liquid semen of Thin-tailed Sheep in tris- egg yolk diluent. This research was carried out on December 7-8, 2023 at the Department of Animal Husbandry, University of Lampung. This study was conducted using a Complete Random Design with 4 treatments and 4 replicates. The treatment is P0: control, P1: 0.6 mg L-Carnitine /100 ml Tris-egg yolk diluent, P2: 1.2 mg L-Carnitine /100 ml Tris-egg yolk diluent and P3: 2.4 mg L- Carnitine /100 ml Tris-egg yolk diluent. Motility, viability, and abnormality data were analyzed at a level of 5%, then motility and viability further tested for orthogonal polynomials. The results of this study showed that the addition of L- Carnitine to the tris-egg yolk had no significant effect on the motility, viability, and abnormality of spermatozoa. The results of orthogonal polynomial motility post-dilution showed the equation Y = 64.752 – 2.2519 X – 0.8996 X2, where X (optimal dose) was 1.25 mg and Y (highest percentage) was 62,35%. The viability post-dilution showed the equation Y = 63.056 + 0.0818 X - 0.6752 X2, where X (optimal dose) was 0.6mg and Y (highest percentage) was 63,3%. The 3-hour storage motility shows the equation Y = 15.623 + 4.4394 X – 2.399 X2, where X (optimal dose) is 0.92mg and Y (highest percentage) is 17,68%. The viability of 3 hours of storage showed the equation Y = 59.479 – 4.8043 X + 1.6989 X2, where X (optimal dose) was 1.41mg and Y (highest percentage) was 56,1%. The results of this study can be concluded that the optimal addition of L- Carnitine for post-dilution motility: 1.25mg/100ml, post-dilution viability: 0.6mg/100ml, motility after 3 hours of storage: 0.92mg/100ml, and viability after 3 hours of storage: 1.41mg/100ml. Keywords: Tris-egg yolk, L-Carnitine, Liquid Semen, Spermatozoa, Thin-Tailed sheep

Jenis Karya Akhir: Skripsi
Subyek: 600 Teknologi (ilmu terapan) > 630 Pertanian dan teknologi yang berkaitan
Program Studi: FAKULTAS PERTANIAN (FP) & PASCASERJANA > Prodi S1 Peternakan
Pengguna Deposit: UPT . Desi Zulfi Melasari
Date Deposited: 10 Sep 2025 07:25
Terakhir diubah: 10 Sep 2025 07:25
URI: http://digilib.unila.ac.id/id/eprint/90472

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