Juliandro Ragil Viaro , Purba (2025) KAJIAN KOMPONEN AKTIF EKSTRAK Lasiodiplodia theobromae SEBAGAI ANTIJAMUR Botrytis cinerea MELALUI PENDEKATAN IN SILICO. FAKULTAS PERTANIAN, UNIVERSITAS LAMPUNG.
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Abstrak (Berisi Bastraknya saja, Judul dan Nama Tidak Boleh di Masukan)
Ekstrak Lasiodiplodia theobromae menghasilkan senyawa metabolit sekunder seperti, sikloheksena, jasmonat, indol dan lainnya sehingga berpotensi sebagai agen antijamur terhadap Botrytis cinerea, penyebab busuk abu-abu pada buah stroberi selama pascapanen. Penelitian ini bertujuan mengidentifikasi komponen aktif kultur L. theobromae yang berpotensi sebagai antijamur B. cinerea secara In silico. Penelitian ini menggunakan metode deskriptif meliputi hasil fraksi terbaik dan diuji LC-MS. Data yang diperoleh dilanjutkan dengan uji In silico dalam bentuk gambar dan tabel. Hasil uji In silico terhadap senyawa hasil LC-MS menggunakan molecular docking dengan protein target ribosom biogenis Ytm1 dan ATPdependent RNA helicase Dbp5 dari B. cinerea, senyawa O-(1,2,3,4,4,5,5,6,6- nonaaminocyclohex-2-en-1-yl)hydroxylamine dari L. theobromae menghambat protein target ribosom biogenesis Ytm1 dengan nilai binding affinity -7,3 kcal/mol melalui ikatan hidrogen dengan residu Lys168A, Lys171A, Ser218A, Ile219A, Ser378A, Ser377A, Asp447A, Phe446A, serta menghambat protein target ATPdependent RNA helicase Dbp5 dengan binding affinity -6,9 kcal/mol melalui ikatan hidrogen dengan residu Tyr181A, Thr221A, dan Gln292A. Hasil penelitian menunjukkan bahwa senyawa O-(1,2,3,4,4,5,5,6,6-nonaaminocyclohex-2-en-1- yl)hydroxylamine dari kultur L. theobromae berpotensi sebagai antijamur alami terhadap B. cinerea. Kata kunci: antijamur, Botrytis cinerea, docking molekuler, in silico, Lasiodiplodia theobromae Extracts of Lasiodiplodia theobromae produced secondary metabolites such as cyclohexene, jasmonate, indole, and others, making it a potential antifungal agent against Botrytis cinerea, the cause of gray mold on strawberries during post-harvest. This study aimed to identify the active components of L. theobromae culture that had potential as an antifungal agents against B. cinerea In silico. The study used a descriptive method covering the results of the best fraction, wich was analyzed using LC-MS. The data obtained were then evaluated through In silico analysis presented in the form of images and tables. In silico testing of the LC-MS compounds using molecular docking with the ribosom biogenesis Ytm1 and ATPdependent RNA helicase Dbp5 from B. cinerea, the compound O-(1,2,3,4,4,5,5,6,6- nonaaminocyclohex-2-en-1-yl)hydroxylamine from L. theobromae inhibited the ribosom biogenesis Ytm1 with a binding affinity value of -7.3 kcal/mol through hydrogen bonds with residues Lys168A, Lys171A, Ser218A, Ile219A, Ser378A, Ser377A, Asp447A, Phe446A and inhibited the target protein ATP-dependent RNA helicase Dbp5 with a binding affinity of -6.9 kcal/mol through hydrogen bonding with residues Tyr181A, Thr221A, and Gln292A. These results of the study indicated that the compound O-(1,2,3,4,4,5,5,6,6-nonaaminocyclohex-2-en-1- yl)hydroxylamine from L. theobromae culture had potential as a natural antifungal agent against B. cinerea. Keywords: antifungal, Botrytis cinerea, in silico, Lasiodiplodia theobromae, moleculer docking
| Jenis Karya Akhir: | Skripsi |
|---|---|
| Subyek: | 600 Teknologi (ilmu terapan) > 630 Pertanian dan teknologi yang berkaitan |
| Program Studi: | FAKULTAS PERTANIAN (FP) & PASCASERJANA > Prodi S1 Teknologi Hasil Pertanian |
| Pengguna Deposit: | 2507372952 Digilib |
| Date Deposited: | 27 Nov 2025 01:58 |
| Terakhir diubah: | 27 Nov 2025 01:58 |
| URI: | http://digilib.unila.ac.id/id/eprint/93808 |
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