Sisi , Indriyani (2025) UJI EFEKTIVITAS Trichoderma asperellum DALAM PENGENDALIAN NEMATODA PURU AKAR (Meloidogyne spp.) SECARA IN VITRO. FAKULTAS PERTANIAN, UNIVERSITAS LAMPUNG.
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Abstrak (Berisi Bastraknya saja, Judul dan Nama Tidak Boleh di Masukan)
Root knot nematodes (Meloidogyne spp.) are important pests that damage plant root systems and are difficult to control using conventional methods. The use of chemical nematicides risks environmental pollution, necessitating environmentally friendly control alternatives, one of which is the use of the biological agent Trichoderma asperellum. This study aimed to determine the effect of spore concentration and exposure duration of T. asperellum on the in vitro hatching of Meloidogyne spp. eggs. The study was conducted in the Plant Pest Science Laboratory and the Biotechnology Laboratory, Faculty of Agriculture, University of Lampung, from May to July 2025. The experiment used a completely randomized design (CRD). The first experiment tested five concentrations of spore suspension dilutions (101, 102, 103, 10, and the control), while the second experiment tested exposure durations (10, 15, 30, and 60 minutes). The results showed that a dilution of 10-3 (with a spore density of 2.7 x 10 spores/mL) was able to reduce egg hatching with juvenile mortality of J2 NPA by 50.90%. Juvenile mortality increased with exposure time, namely 85.37% at 10 minutes and 99.83% at 60 minutes. The results of this study concluded that a spore density of 2.7 x 10 'spores/mL with a minimum exposure of 30-60 minutes effectively reduced the hatching rate of Meloidogyne spp. eggs and has the potential to be applied as a biological control agent for plant parasitic nematodes. Keyworsds: Exposure duration, spore, Meloidogyne spp., biological control, Trichoderma asperellum Nematoda puru akar (Meloidogyne spp.) merupakan hama penting yang merusak sistem perakaran tanaman dan sulit dikendalikan dengan cara konvensional. Penggunaan nematisida kimia berisiko mencemari lingkungan, sehingga diperlukan alternatif pengendalian yang ramah lingkungan, salah satunya menggunakan agens hayati Trichoderma asperellum. Penelitian ini bertujuan untuk mengetahui pengaruh konsentrasi spora dan durasi paparan T. asperellum terhadap penetasan telur Meloidogyne spp. secara in vitro. Penelitian dilaksanakan di Laboratorium Ilmu Hama Tumbuhan dan Laboratorium Bioteknologi Fakultas Pertanian Universitas Lampung dari Mei–Juli 2025. Percobaan menggunakan rancangan acak lengkap (RAL). Percobaan pertama menguji lima konsentrasi suspensi spora pengenceran (10⁻¹, 10⁻², 10⁻³, 10⁻⁵, dan kontrol), sedangkan percobaan kedua menguji durasi paparan yaitu (10, 15, 30, dan 60 menit). Hasil penelitian menunjukkan pengenceran 10⁻² (dengan kerapatan spora 2,7 × 10⁵ spora/mL ) mampu menurunkan penetasan telur dengan mortalitas juvenil J2 NPA sebesar 50,90%. Mortalitas juvenil meningkat seiring waktu paparan, yaitu 85,37% pada 10 menit dan 99,83% pada 60 menit. Hasil penelitian ini menyimpulkan, kerapatan spora 2,7 × 10⁵ spora/mL dengan paparan minimal 30– 60 menit efektif menurunkan tingkat penetasan telur Meloidogyne spp. dan berpotensi diaplikasikan sebagai agensia pengendali hayati nematoda parasit tumbuhan. Kata kunci: Durasi paparan, konsentrasi spora, Meloidogyne spp., pengendalian hayati, Trichoderma asperellum.
| Jenis Karya Akhir: | Skripsi |
|---|---|
| Subyek: | 600 Teknologi (ilmu terapan) > 630 Pertanian dan teknologi yang berkaitan |
| Program Studi: | FAKULTAS PERTANIAN (FP) & PASCASERJANA > Prodi S1 Proteksi Tanaman |
| Pengguna Deposit: | 2507836904 Digilib |
| Date Deposited: | 05 Dec 2025 06:41 |
| Terakhir diubah: | 05 Dec 2025 06:41 |
| URI: | http://digilib.unila.ac.id/id/eprint/93947 |
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