KIDUNG , RAMADHAN (2025) EVALUASI PERAN RAGI DAN Bacillus sp. TERHADAP PERFORMA BUDI DAYA, IMUNITAS NON-SPESIFIK SERTA DETEKSI PENYAKIT PADA UDANG VANAME (Litopenaus vannamei). FAKULTAS PERTANIAN, UNIVERSITAS LAMPUNG.
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Abstrak (Berisi Bastraknya saja, Judul dan Nama Tidak Boleh di Masukan)
Udang vaname (Litopenaeus vannamei) merupakan salah satu komoditas utama dalam akuakultur Indonesia karena produktivitasnya yang tinggi dan daya adaptasi yang baik. Namun, budidaya intensif sering dihadapkan pada ancaman penyakit infeksius seperti Acute Hepatopancreatic Necrosis Disease (AHPND) dan Enterocytozoon hepatopenaei (EHP). Penelitian ini bertujuan untuk meng- evaluasi efektivitas penambahan ragi dan Bacillus sp. dalam pakan terhadap performa pertumbuhan, imunitas non-spesifik dan deteksi penyakit AHPND dan EHP. Penelitian dilakukan selama 56 hari dengan dua perlakuan: penambahan ragi 3 g/kg (P1) dan Bacillus sp. 2 g/kg (P2), masing-masing dengan tiga ulangan. Parameter yang diamati meliputi rerata berat tubuh, rerata pertumbuhan harian, tingkat kelansgungan hidup, rasio konversi pakan, total haemocyte count, differen- tial haemocyte count, deteksi AHPND dan EHP dengan sampel sedimen dan udang menggunakan metode Polymerase Chain Reaction (PCR) dan biomassa. Hasil penelitian menunjukkan bahwa perlakuan ragi (3 g/kg) dan Bacillus sp. (2 g/kg) tidak berbeda nyata terhadap berat tubuh, pertumbuhan harian, kelang- sungan hidup, dan komposisi diferensial hemosit udang vaname (p>0,05). Namun, Bacillus sp. memberikan rasio konversi pakan lebih rendah (1,12 ± 0,08) dibanding ragi (1,68 ± 0,31) dan biomassa lebih tinggi (1099 ± 121 kg) berban- ding (805 ± 31,56 kg) (p<0,05). Sebaliknya, ragi meningkatkan total haemocyte count lebih tinggi (8,9 ± 0,3 × 106 sel/ml) dibanding Bacillus sp. (7,8 ± 0,6 × 106 sel/ml) (p<0,05). Deteksi PCR menemukan AHPND dan EHP dengan jumlah salinan rendah pada DOC 28–42. Kata kunci: AHPND, Bacillus sp., EHP, Ragi, Udang Vaname Pacific white shrimp (Litopenaeus vannamei) is one of the main commo- dities in Indonesian aquaculture due to its high productivity and adaptability. However, intensive farming is often challenged by infectious diseases such as Acute Hepatopancreatic Necrosis Disease (AHPND) and Enterocytozoon hepatopenaei (EHP). This study aimed to evaluate the effec-tiveness of dietary supplementation with yeast and Bacillus sp. on growth performance, non-specific immunity, and the detection of AHPND and EHP. The experiment was conducted for 56 days using two treatments: yeast supplemen-tation at 3 g/kg (P1) and Bacillus sp. at 2 g/kg (P2), each with three replicates. Parameters observed included mean body weight, daily growth rate, survival rate, feed conversion ratio (FCR), total haemocyte count (THC), differential haemocyte count (DHC), disease detection of AHPND and EHP from sediment and shrimp samples using Polymerase Chain Reaction (PCR), and final biomass. The results showed no significant differences (p>0.05) between yeast and Bacillus sp. treatments in mean body weight, daily growth rate, survival rate, or DHC. However, Bacillus sp. produced a significantly lower FCR (1.12 ± 0.08) compared to yeast (1.68 ± 0.31) and a higher biomass (1099 ± 121 kg) compared to yeast (805 ± 31.56 kg) (p<0.05). Conversely, yeast significantly increased THC (8.9 ± 0.3 × 106 cells/ml) compared to Bacillus sp. (7.8 ± 0.6 × 106 cells/ml) (p<0.05). PCR detection revealed low copy numbers of AHPND and EHP, first identified at DOC 28–42. Keywords: AHPND, Bacillus sp., EHP, Pacific White Shrimp, Yeast
| Jenis Karya Akhir: | Skripsi |
|---|---|
| Subyek: | 500 ilmu pengetahuan alam dan matematika > 590 Ilmu hewan (zoologi) 600 Teknologi (ilmu terapan) > 630 Pertanian dan teknologi yang berkaitan |
| Program Studi: | FAKULTAS PERTANIAN (FP) & PASCASERJANA > Prodi S1 Perikanan dan Kelautan |
| Pengguna Deposit: | A.Md Cahya Anima Putra . |
| Date Deposited: | 29 Jan 2026 04:14 |
| Terakhir diubah: | 29 Jan 2026 04:14 |
| URI: | http://digilib.unila.ac.id/id/eprint/95202 |
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