Eki Panji , Permana (2025) KAJIAN KOMPONEN AKTIF KULTUR Lasiodiplodia theobromae UNTUK MEMPERCEPAT KEMATANGAN BUAH PISANG AMBON (Musa paradisiaca L.). FAKULTAS PERTANIAN, UNIVERSITAS LAMPUNG.
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Abstract
Banana (Musa paradisiaca L.) is a climacteric fruit that requires specific postharvest handling to maintain its quality. This study aimed to determine the optimal concentration of active compounds derived from the culture of Lasiodiplodia theobromae in accelerating the ripening of Ambon bananas. A Completely Randomized Block Design (CRBD) was applied with seven concentration levels (0 ppm, 0.5 ppm, 1 ppm, 1.5 ppm, 2 ppm, 2.5 ppm, and 3 ppm), each repeated three times. Extraction was performed using ethyl acetate and fractionated using column chromatography. Identification of active compounds was conducted using LC-MS, resulting in the detection of four compounds, namely 1,1,2-triaminoguanidine, tetradecylamine, O-(1,2,3,4,4,5,5,6,6-nonaaminocyclohex-2-en-1-yl) hydroxylamine, and 9,10-dioctyloctadecan-9-amine. Observed parameters included weight loss, color (L*, a*, b*), firmness, sugar content, and moisture content. The results showed that concentration of 2.5 ppm was most effective in accelerating the ripening of Ambon bananas, with a weight loss of 5,37%, L* value of 62,55 (bright), a* value of –-8,95 (green), b* value of 41,62 (yellowish), firmness of 4.765,00 gf, sugar content of 14,33%, and moisture content of 71,16%. Keyword: Lasiodiplodia theobromae, Ambon banana, rippening fruit, active compounds, LC-MS Pisang (Musa paradisiaca L.) merupakan buah klimakterik yang memerlukan penanganan pascapanen khusus untuk menjaga kualitasnya. Penelitian ini bertujuan untuk mengetahui konsentrasi terbaik komponen aktif dari kultur Lasiodiplodia theobromae dalam mempercepat pematangan buah pisang ambon. Penelitian menggunakan Rancangan Acak Kelompok Lengkap (RAKL) dengan tujuh taraf perlakuan konsentrasi (0 ppm, 0,5 ppm, 1 ppm, 1,5 ppm, 2 ppm, 2,5 ppm, dan 3 ppm) dengan tiga kali ulangan. Ekstraksi dilakukan menggunakan etil asetat dan difraksinasi dengan kolom kromatografi. Identifikasi komponen aktif dilakukan menggunakan LC-MS yang menghasilkan empat senyawa teridentifikasi yaitu 1,1,2-triaminoguanidine,tetradecylamine,O-(1,2,3,4,4,5,5,6,6nonaaminocyclohex- 2-en-1-yl) hydroxylamine, dan 9,10-dioctyloctadecan-9-amine. Parameter yang diamati meliputi susut bobot, warna (L*, a*, b*), tingkat kekerasan, kadar gula, dan kadar air. Hasil penelitian menunjukkan bahwa konsentrasi 1,5 ppm merupakan konsentrasi terbaik dalam mempercepat pematangan buah pisang ambon susut bobot 5,37%, nilai warna L* 62,55 (cerah), a* -8,95 (hijau), b* 41,62 (kekuningan), tingkat kekerasan 4.765,00 gf, kadar gula 14,33%, dan kadar air 71,16%. Kata kunci: Lasiodiplodia theobromae, pisang ambon, pematangan buah, komponen aktif, LC-MS
| Item Type: | Other |
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| Subjects: | ?? 630 ?? |
| Divisions: | Fakultas Pertanian > Prodi Teknologi Hasil Pertanian |
| Depositing User: | 2507221145 Digilib |
| Date Deposited: | 30 Jan 2026 07:52 |
| Last Modified: | 30 Jan 2026 07:52 |
| URI: | http://digilib.unila.ac.id/id/eprint/95246 |
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