?url_ver=Z39.88-2004&rft_val_fmt=info%3Aofi%2Ffmt%3Akev%3Amtx%3Adc&rft.title=Uji+Imunogenesitas+Vaksin+Inaktif+Whole+Cell%0D%0AAeromonas+salmonicida+Pada+Ikan+Mas%0D%0A(Cyprinus+carpio)&rft.creator=0814111066%2C+ZULFIKAR+SAFESKA+RONAPATI&rft.subject=+&rft.description=Abstrak%0D%0A%0D%0AAeromonas+salmonicida+merupakan+bakteri+pathogen+penyebab+penyakit+carp%0D%0Aerytrodermatitis+pada+ikan+mas.+Salah+satu+solusi+dalam+pencegahan+penyakit+ini%0D%0Aadalah+vaksinasi.+Tujuan+dari+penelitian+ini+adalah+mengetahui+tingkat%0D%0Aimunogenisitas+vaksin+inaktif+whole+cell+A.+salmonicida+pada+ikan+mas+serta%0D%0Amencari+metode+vaksinasi+yang+terbaik.+Vaksin+inaktif+dibuat+dengan+cara%0D%0Adiinaktifasi+dengan+formalin+1%25+dan+diinkubasikan+selama+24+jam+pada+suhu%0D%0Aruang.+Vaksinasi+diujikan+pada+10+ekor+ikan%2Fmetode+vaksinasi+yaitu+dengan+cara%0D%0Asuntik+melalui+intraperitoneal+(107+sel%2Fikan)%2C+oral+(107+sel%2Fikan)%2C+perendaman+(107%0D%0Asel%2Fml+air%2C+lama+perendaman+30+menit)+dan+ikan+tanpa+divaksinasi+sebagai+kontrol.%0D%0AVaksinasi+dilakukan+sebanyak+2+kali%2C+booster+dilakukan+7+hari+setelah+vaksinasi+I%0D%0Adengan+dosis+dan+metode+vaksinasi+yang+sama.+Pengamatan+titer+antibodi%0D%0Adilakukan+tiga+kali+yaitu%2C+sebelum+vaksinasi%2C+7+hari+setelah+vaksinasi+I%2C+dan+7+hari%0D%0Asetelah+booster.+Parameter+kualitas+air+yang+diukur+adalah+oksigen+terlarut%2C+pH%0D%0Adan+suhu+sebagai+parameter+pendukung.+Hasil+penelitian+diperoleh+rata+%E2%80%93+rata+titer%0D%0Aantibodi+untuk+semua+metode+sebelum+vaksinasi+sebesar+1%2F6%2C+setelah+vaksinasi+I%0D%0Adan+booster+terjadi+peningkatan+menjadi+1%2F58.67+dan+1%2F85.33+pada+metode+suntik%2C%0D%0A1%2F42.67+dan+1%2F64+untuk+metode+oral%2C+dan+1%2F24+dan+1%2F42.67+pada+perendaman.%0D%0ASedangkan+pada+ikan+kontrol+tanpa+vaksinasi+terjadi+peningkatan+namun+tidak%0D%0Asignifikan+yaitu+1%2F9.33+dan+1%2F18.67.+Metode+vaksinasi+terbaik+adalah+metode%0D%0Asuntik+secara+i.p+yang+menunjukkan+rata+%E2%80%93+rata+hasil+titer+antibodi+tertinggi.+Hasil%0D%0Apengukuran+kualitas+air+selama+penelitian+masih+berada+dalam+kisaran+normal%0D%0Ahidup+ikan+uji.%0D%0AKata+kunci+%3A+Aeromonas+salmonicida%2C+ikan+mas%2C+titer+antibodi%2C+vaksin+inaktif.%0D%0A%0D%0A%0D%0A%0D%0A%0D%0AAbstract%0D%0A%0D%0AAeromonas+salmonicida+is+bacterial+pathogen+that+caused+carp+erytrodermatitis+in%0D%0Acommon+carp.+One+preventive+action+to+protect+this+infection+was+by+vaccination.%0D%0AThe+aims+of+this+experiment+were+to+determine+the+immunogenicity+of+inactivated%0D%0Avaccine+A.+salmonicida+whole+cell+in+common+carp+and+the+best+administration%0D%0Amethod+of+vaccine.+Vaccine+was+produced+by+inactivated+A.+salmonicida+with+1%0D%0A%25+formaldehyde+and+incubated+for+24+hours+in+room+temperature.+Vaccine+was%0D%0Atested+in+10+fish%2Fvaccination+method+by+injection+intra+peritoneal+(107+cell%2Ffish)%2C%0D%0Aoral+(107+cell%2Ffish)%2C+immersion+(107+cell%2Fml+for+30+minute)+and+fish+that+had+not%0D%0Avaccinated+as+a+control.+Booster+was+conducted+in+7th+days+after+first+vaccination%0D%0Awith+same+both+dosage+and+method.+Titer+antibody+was+evaluated+in+three+times%0D%0Ai.e.+before+vaccination%2C+7th+days+after+first+vaccination%2C+and+7th+days+after+booster.%0D%0AWater+quality+such+as+dissolved+oxygen%2C+pH+and+water+temperature+was+measured%0D%0Aas+supported+parameters.+The+results+of+the+experiment+showed+that+average+of%0D%0Atiter+antibody+for+all+method+before+vaccination+was+1%2F6.+However%2C+titer+antibody%0D%0Aafter+first+vaccination+and+booster+increased+to+1%2F58.67+and+1%2F85.33+for+i.p%0D%0Ainjection%2C+1%2F42.67+and+1%2F64+for+orally%2C+1%2F24+and+1%2F42.67+for+immerse%2C+respectively.%0D%0ABeside+that%2C+there+was+no+significantly+increasing+of+titer+antibody+for+fish+controls%0D%0Athat+was+1%2F9.33+and+1%2F18.67.+Vaccination+method+by+i.p+injection+was+the+best%0D%0Amethod+that+showed+the+highest+immunogenicity+of+vaccine.+Water+quality%0D%0Aparameters+for+this+experiment+still+in+optimum+range+for+common+carp+live%0D%0Amedium.%0D%0AKey+words%3A+Aeromonas+salmonicida%2C+common+carp%2C+inactivated+vaccine%2C+titer+antibody.&rft.date=2012-04-09&rft.type=Skripsi&rft.type=PeerReviewed&rft.format=text&rft.identifier=http%3A%2F%2Fdigilib.unila.ac.id%2F12381%2F1%2FABSTRACT.pdf&rft.format=text&rft.identifier=http%3A%2F%2Fdigilib.unila.ac.id%2F12381%2F2%2FABSTRAK.pdf&rft.format=text&rft.identifier=http%3A%2F%2Fdigilib.unila.ac.id%2F12381%2F3%2Fcover%25202.pdf&rft.format=text&rft.identifier=http%3A%2F%2Fdigilib.unila.ac.id%2F12381%2F4%2Fcover.pdf&rft.format=text&rft.identifier=http%3A%2F%2Fdigilib.unila.ac.id%2F12381%2F5%2FDAFTAR%2520GAMBAR.pdf&rft.format=text&rft.identifier=http%3A%2F%2Fdigilib.unila.ac.id%2F12381%2F6%2FDAFTAR%2520ISI.pdf&rft.format=text&rft.identifier=http%3A%2F%2Fdigilib.unila.ac.id%2F12381%2F7%2FIV.pdf&rft.format=text&rft.identifier=http%3A%2F%2Fdigilib.unila.ac.id%2F12381%2F8%2FMETODOLOGI.pdf&rft.format=text&rft.identifier=http%3A%2F%2Fdigilib.unila.ac.id%2F12381%2F9%2FPENDAHULUAN.pdf&rft.form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