%A Heri Gunawan 0714111038
%J Digital Library
%T AKTIFITAS ANTIBAKTERI EKSTRAK BUAH MANGROVE Rhizophora sp.
TERHADAP BAKTERI Vibrio spp.
%X Abstrak

Vibrio spp. merupakan salah satu bakteri pathogen pada ikan dan udang.
Penanggulangan penyakit vibriosis dengan antibiotik mulai ditinggalkan, karena
berbagai dampak negatif yang muncul. Berbagai macam bahan alami dilaporkan
memiliki kandungan senyawa antibakteri. Mangrove jenis Rhizophora sp. merupakan
salah satu bahan alami yang berpotensi sebagai sumber obat-obatan dan senyawa
antibakteri. Penelitian ini bertujuan untuk mengetahui aktifitas antibakteri ekstrak
buah mangrove Rhizophora sp. sebagai antibakteri terhadap bakteri Vibrio spp (V.
alginolyticus, V.vulnivicus, V. parahaemolyticus, dan V. ordalii). Buah Rhizopora sp. di
ekstrak dengan 3 jenis pelarut berdasarkan sifat kepolaran yaitu metanol (polar), etil
asetat (semi polar), dan heksana (non polar). Ekstrak diuji aktifitas antibakterinya
terhadap empat spesies V. alginolyticus, V.vulnivicus, V. parahaemolyticus, dan V. ordalii
dengan tahapan uji sensitifitas, uji zona hambat, uji MIC, uji MBC, uji toksisitas dan uji
inhibition time course. Hasil uji antibakteri menunjukan bahwa ekstrak buah
mangrove Rhizophora sp. yang diekstrak dengan bahan pelarut etil asetat, memiliki
potensi paling besar dalam menghambat pertumbuhan bakteri V. Alginolyticus
dengan nilai MIC 300 ppm dan nilai MBC sebesar 400 ppm. Uji Toksisitas (Brine
Shrimp Lethality Test) menunjukan nilai toksisitas ekstrak buah mangrove
Rhizophora sp. yang diekstrak dengan bahan pelarut etil asetat sebesar 859 mg/l hal
ini menunjukan bahwa ekstrak buah mangrove Rhizopora sp. bersifat toksik dan tidak
dapat digunakan pada uji in vivo. Hasil Uji Inhibition Time Course pada jam ke 6
ekstrak buah Rhizopora sp. mulai dapat menghambat bakteri V. alginolyticus.
Kata kunci : antibakteri, ekstrak Rhizophora sp. Vibrio spp.




Abstract

Vibrio is one of pathogenic bacteria on fish and shrimp. Disease handling Vibriosis
with stibiotics has been abandoned, due to various negative impacts that have showed
up. A wide variety of natural products was reported to have antibacterial component.
Rhizophora sp. is one of natural products which was potential as a source of drugs
and component of antibacteria. Research was aimed to know antibacterial activity in
fruit extract of Rhizophora sp. as an antibacteria against Vibrio spp. ( V. alginolyticus,
V. vulnivicus, V. parahaemolyticus, and V. ordalii ). Fruits of Rhizopora sp. were
extracted by 3 sets of solvents based on the level of their polarity, which were
methanol (polar), ethyl acetate (semi polar), and hexane (non-polar). The extract were
tested their antibacterial activities against four species V. alginolyticus, V.vulnivicus,
V. parahaemolyticus, and V. ordalii. The tests were sensitivity test, inhibitory test,
MIC test, MBC test, toxicity test and inhibition time course test. Antibacterial test
showed that extract of Rhizophora sp. fruits dissolved by ethyl acetate was the most
potential in inhibiting the growth of bacteria V. alginolyticus with the value of the
MIC was 300 ppm and MBC was 400 ppm. Toxicity Test (Brine Shrimp Lethality
Test) showed that value of toxicity from Rhizophora sp. fruits extracted with ethyl
acetate was 859 mg/l. This leaded that fruit extract of Rhizopora sp. was toxic and
can not be used in in vivo experiment. The test inhibition time course showed that in
6 hours extract of Rhizopora sp. fruits began to inhibit V. alginolyticus.
Keyword: antibacterial, extracts Rhizophora sp., Vibrio spp.
%D 2012
%L eprints12491