?url_ver=Z39.88-2004&rft_val_fmt=info%3Aofi%2Ffmt%3Akev%3Amtx%3Adc&rft.title=PENYIMPANAN+VAKSIN+INAKTIF+WHOLE%0D%0ACELL+Aeromonas+salmonicida+DENGAN%0D%0APENAMBAHAN+GLISEROL&rft.creator=0814111012%2C+RINDA+ARYANI+PUTRI&rft.subject=+&rft.description=Abstrak%0D%0A%0D%0AVaksin+inaktif+whole+cell+A.+salmonicida+memiliki+tingkat+imunogenesitas+yang%0D%0Acukup+tinggi+pada+ikan+mas+(Cyprinus+carpio+L).+Untuk+menjaga+stabilitas%0D%0Aimunogenesitas+vaksin+tersebut%2C+perlu+dilakukan+penyimpanan+yang+baik.+Gliserol%0D%0Adiketahui+mampu+menjaga+keutuhan+sel+dalam+penyimpanan+dingin.+Tujuan+dari%0D%0Apenelitian+ini+yaitu+untuk+mengetahui+dosis+gliserol+terbaik+dalam+penyimpanan%0D%0Avaksin+whole+cell+A.+salmonicida.Vaksin+diinaktifasi+dengan+formalin+1%25+dan%0D%0Adiinkubasikan+selama+24+jam+pada+suhu+ruang.+Kepadatan+vaksin+dihitung+lalu%0D%0Aditambahkan+gliserol+dengan+dosis+0%2C25%25%2C+0%2C50%25%2C+0%2C75%25+dan+2+perlakuan+lain%0D%0Asebagai+kontrol+positif+(vaksin+tanpa+gliserol)+dan+negatif+(tanpa+vaksin%2C+berisi%0D%0APBS).+Vaksin+diujikan+pada+10+ekor+ikan+mas+per+perlakuan+dengan+cara%0D%0Apenyuntikan+intraperitoneal+(107+sel%2Fikan).+Seminggu+berikutnya+dilakukan%0D%0Abooster+dengan+metode+dan+dosis+yang+sama.+Vaksinasi+dan+booster+dilakukan%0D%0Apada+penyimpanan+vaksin+0+hari+dan+30+hari+dengan+menggunakan+ikan+mas+yang%0D%0Aberbeda.+Titer+antibodi+diamati+sebelum+vaksinasi%2C+seminggu+setelah+vaksinasi%2C%0D%0Adan+seminggu+setelah+booster%2C+baik+pada+penyimpanan+0+hari+dan+30+hari.%0D%0AParameter+pendukung+kualitas+air+akuarium+diamati+setiap+hari+meliputi+DO%2C+pH%0D%0Adan+suhu.+Hasil+penelitian+menunjukkan+tingkat+titer+antibodi+tertinggi+pada%0D%0Avaksin+dengan+penambahan+dosis+gliserol+0%2C50%25+dengan+rata-rata+256+kali%0D%0Apengenceran+pada+uji+penyimpanan+vaksin+30+hari.+Hasil+pengukuran+kualitas+air%2C%0D%0Asecara+umum+masih+berada+dalam+kisaran+normal+untuk+kelangsungan+hidup+ikan%0D%0Amas.+Kesimpulan+dari+penelitian+ini+yaitu+dosis+gliserol+terbaik+dalam%0D%0Amempertahankan+tingkat+imunogenesitas+vaksin+A.+salmonicida+adalah+vaksin%0D%0Adengan+penambahan+gliserol+0%2C50%25.%0D%0AKata+kunci+%3A+Aeromonas+salmonicida%2C+gliserol%2C+vaksin%2C+titer+antibodi%2C+ikan+mas%0D%0A%0D%0A%0D%0A%0D%0A%0D%0A%0D%0A%0D%0A%0D%0AAbstract%0D%0A%0D%0AInactive+whole+cell+vaccine+of+A.+salmonicida+has+has+the+high+level+of%0D%0Aimmunogenecity+on+common+carp.+To+maintain+the+stability+of+the+vaccine%0D%0Aimunogenecity%2C+the+best+storage+was+needed.+Glycerol+has+been+known+to+be+able%0D%0Ato+maintain+the+integrity+of+the+cell+in+cold+storage.+The+purpose+of+this+research%0D%0Awas+to+know+the+best+dosage+of+glycerol+in+the+storage+of+inactive+whole+cell%0D%0Avaccine+of+A.+salmonicida.+Vaccine+was+inactived+with+1%25+formaline+and%0D%0Aincubated+for+24+hours+at+room+temperature.+The+density+of+vaccine+was+counted%0D%0Aand+then+vaccine+was+added+with+0%2C25%25%2C+0%2C50%25%2C+0%2C75%25+of+glycerol.+Two+other%0D%0Atreatments+were+used+as+positive+(vaccine+without+glycerol)+and+negative+(without%0D%0Avaccine%2C+contains+PBS)+controls.+The+vaccine+was+tested+on+10+fish%2Ftreatment+by%0D%0Aintraperitoneal+injection+(107cell%2Ffish).+Vaccination+and+booster+were+done+in+0%0D%0Aday+and+30+days+of+storage+using+a+different+fish.+Observation+of+titer+antibody%0D%0Awas+done+before+vaccination%2C+a+week+after+vaccination+and+a+week+after+the%0D%0Abooster.+Water+quality+as+supporting+parameter+was+observed+every+day%2C+including%0D%0ADO%2C+pH+and+temperature.+As+a+results%2C+the+highest+level+of+titer+antibody+was%0D%0Areached+on+vaccine+with+the+adding+dosage+of+0%2C50%25+of+glycerol+with+the+average%0D%0A256+times+dilution+on+the+storage+of+vaccine+30+days.+Water+quality+during+this%0D%0Aresearch+that+was+still+in+normal+for+common+carp+life.+In+conclusion%2C+the+best%0D%0Adosage+to+keep+the+level+of+immonugenecity+of+A.+salmonicida+vaccine+was+the%0D%0Aadditional+0%2C50%25+of+glycerol.%0D%0AKey+words%3A+Aeromonas+salmonicida%2C+glycerol%2C+formaline+killed+vaccine%2C+titer%0D%0Aantibody%2C+common+carp&rft.date=2012-02-03&rft.type=Skripsi&rft.type=PeerReviewed&rft.format=text&rft.identifier=http%3A%2F%2Fdigilib.unila.ac.id%2F12500%2F1%2FABSTRACT.pdf&rft.format=text&rft.identifier=http%3A%2F%2Fdigilib.unila.ac.id%2F12500%2F2%2FABSTRAK.pdf&rft.format=text&rft.identifier=http%3A%2F%2Fdigilib.unila.ac.id%2F12500%2F3%2Fcover.pdf&rft.format=text&rft.identifier=http%3A%2F%2Fdigilib.unila.ac.id%2F12500%2F4%2Fcover2.pdf&rft.format=text&rft.identifier=http%3A%2F%2Fdigilib.unila.ac.id%2F12500%2F4%2FDAFTAR%2520GAMBAR.pdf&rft.format=text&rft.identifier=http%3A%2F%2Fdigilib.unila.ac.id%2F12500%2F5%2FDAFTAR%2520ISI.pdf&rft.format=text&rft.identifier=http%3A%2F%2Fdigilib.unila.ac.id%2F12500%2F6%2FDAFTAR%2520ISTILAH.pdf&rft.format=text&rft.identifier=http%3A%2F%2Fdigilib.unila.ac.id%2F12500%2F7%2FDAFTAR%2520PUSTAKA.pdf&rft.format=text&rft.identifier=http%3A%2F%2Fdigilib.unila.ac.id%2F12500%2F8%2FI.pdf&rft.format=text&rft.identifier=http%3A%2F%2Fd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