creators_name: Junaidi Permana, 1117011027
creators_id: junaidipermana@gmail.com
type: other
datestamp: 2015-12-11 03:29:54
lastmod: 2015-12-11 03:29:54
metadata_visibility: show
title: ISOLASI DAN KARAKTERISASI SENYAWA ANTOSIANIN DARI DAUN ADAM HAWA (Rhoeo discolor L. Her)
ispublished: pub
subjects: Q
full_text_status: restricted
abstract: Anthocyanins has been isolated from adam hawa (Rhoeo discolor L. Her) leaves, using maceration method with water : HCl (100: 0.1 v/v) as a solvent. Extract was concentrated using freeze drying method, and it was separated on Sephadex LH-20 coloumn. Fraction 2-5 from elution showed one spot on TLC chromatogram using silica GF245 as adsorbent, and buthanol: acetic acid: water (4: 1: 5 v/v) as eluent at Rf 0.42. This fraction was analized with UV-Vis and it was found that the spot has maximum wavelength at 535.0 nm, and it predicted as cyanidin-3-galactose or peonidin-3-glucose. The compound was found to exhibit 80.23891% antioxidant activity based on DPPH (2,2-diphenyl-1-picrylhydrazil) method.
Key Words : Anthocyanins, Rhoeo discolor L. Her, Sephadex LH-20, Antioxidant, and DPPH

Antosianin telah diisolasi dari daun adam hawa (Rhoeo discolor L. Her), menggunakan metode maserasi dengan pelarut akuades : HCl (100: 0,1 v/v). Ekstrak dipekatkan dengan menggunakan metode freeze drying, kemudian dilakukan pemisahan menggunakan metode kromatografi kolom sefadeks LH-20. Hasil elusi pada fraksi 2-5 menunjukkan adanya 1 noda pada kromatogram KLT dengan menggunakan adsorben silika GF245, eluen butanol: asam asetat: air (4: 1: 5 v/v) pada Rf 0,42. Fraksi tersebut kemudian dianalisis menggunakan spektrofotometri UV-Vis, diperoleh panjang gelombang maksimal pada 534.0 nm, dan diperkirakan sebagai sianidin-3-galaktosa atau peonidin-3-glukosa. Fraksi antosianin hasil isolasi juga memiliki aktivitas antioksidan sebesar 80,23891% berdasarkan metode DPPH (2,2-difenil-1-pikrilhidrazil).
Kata Kunci : Antosianin, Rhoeo discolor L. Her, Sefadeks LH-20, Antioksidan, dan DPPH.
date: 2015-11-23
date_type: published
publisher: MIPA
place_of_pub: Universitas Lampung
institution: Universitas Lampung
department: FMIPA
thesis_type: other
citation:   Junaidi Permana, 1117011027  (2015) ISOLASI DAN KARAKTERISASI SENYAWA ANTOSIANIN DARI DAUN ADAM HAWA (Rhoeo discolor L. Her).  MIPA, Universitas Lampung.     
document_url: http://digilib.unila.ac.id/15591/1/COVER%20LUAR.pdf
document_url: http://digilib.unila.ac.id/15591/2/ABSTRACT.pdf
document_url: http://digilib.unila.ac.id/15591/3/ABSTRAK.pdf
document_url: http://digilib.unila.ac.id/15591/4/COVER%20DALAM.pdf
document_url: http://digilib.unila.ac.id/15591/5/LEMBAR%20PERSETUJUAN.pdf
document_url: http://digilib.unila.ac.id/15591/6/LEMBAR%20PENGESAHAN.pdf
document_url: http://digilib.unila.ac.id/15591/7/RIWAYAT%20HIDUP.pdf
document_url: http://digilib.unila.ac.id/15591/8/MOTO.pdf
document_url: http://digilib.unila.ac.id/15591/9/PERSEMBAHAN.pdf
document_url: http://digilib.unila.ac.id/15591/10/SANWACANA.pdf
document_url: http://digilib.unila.ac.id/15591/11/DAFTAR%20ISI.pdf
document_url: http://digilib.unila.ac.id/15591/12/DAFTAR%20TABEL.pdf
document_url: http://digilib.unila.ac.id/15591/13/DAFTAR%20GAMBAR.pdf
document_url: http://digilib.unila.ac.id/15591/14/BAB%20I.pdf
document_url: http://digilib.unila.ac.id/15591/15/BAB%20II.pdf
document_url: http://digilib.unila.ac.id/15591/16/BAB%20III.pdf
document_url: http://digilib.unila.ac.id/15591/17/BAB%20IV.pdf
document_url: http://digilib.unila.ac.id/15591/18/BAB%20V.pdf
document_url: http://digilib.unila.ac.id/15591/19/DAFTAR%20PUSTAKA.pdf
document_url: http://digilib.unila.ac.id/15591/20/LAMPIRAN-LAMPIRAN.pdf