creators_name: 0924051018, REFIARIOEN
type: article
datestamp: 2016-01-13 06:49:18
lastmod: 2016-01-13 06:49:18
metadata_visibility: show
title: STUDY OF ALKALI AND ENZYMATIC PRETREATMENTS ON
SUGARCANE BAGASSE TO PRODUCE REDUCED SUGAR
ispublished: pub
subjects: General
full_text_status: public
abstract: Abstrak

Sugarcane bagasse is one of solid waste from sugar factories. It contains
lignocelluloses that can be hydrolyzed into reduced sugar. Objectives of this
study were to find out (1) the best condition (alkali concentration, lignin
degradation time and temperature) of bagasse lignin degradation, and (2) the best
substrate concentration and enzymatic incubation time for hydrolyzing baggase
holocelluloses into reduced sugar.
This study was performed into two phases, namely lignin degradation and
enzymatic hydrolysis. During the former phase, sugarcane bagasse was immersed
in alkali (NaOH, H2O2) solution at a variety of concentrations for a variety of
times and temperatures. After degradation, samples were taken and analyzed for
degree of lignin degradation. During the latter phases, 3.75% - 6.25% baggase
lignocelluloses were hydrolyzed with 10 FPU cellulase enzyme for 0 – 24 hours at
pH 4.8, 50oC, and 200 rpm. After hydrolyzation process, samples were taken and
analyzed for determining their reduced sugar contents. Collected data were
analyzed descriptively and presented graphycally. Results of this study shown
that (1) the best condition for lignin degradation (> 99% lignin degraded) was
immersion of the sugarcane baggase into 1.0 M NaOH solution at 121 oC for 15
minutes, and (2) the best treatment for sugarcane bagasse enzymataic hydrolysis
was a substrate concentration of 6.25% and a hydrolysis time of 18 hours. The
hydrolysis treatment yielded a reduced sugar content of 19.29g/L and a bagasse
carbon conversion of 12,4%.
Key words: alkali, bagasse, pretreatment, NaOH, enzymatic hydrolysis.
date: 2012-01-13
date_type: published
publication: Digital Library
refereed: TRUE
citation:   0924051018, REFIARIOEN  (2012) STUDY OF ALKALI AND ENZYMATIC PRETREATMENTS ON SUGARCANE BAGASSE TO PRODUCE REDUCED SUGAR.  Digital Library.       
document_url: http://digilib.unila.ac.id/17337/1/0924051018-abstract.pdf
document_url: http://digilib.unila.ac.id/17337/2/0924051018-abstrak.pdf
document_url: http://digilib.unila.ac.id/17337/3/0924051018-kesimpulan.pdf
document_url: http://digilib.unila.ac.id/17337/4/0924051018-pendahuluan.pdf