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VALIDASI METODE MATRIX SOLID PHASE DISPERSION (MSPD)SPEKTROFOTOMETRI UV UNTUK ANALISIS RESIDU TETRASIKLIN DALAM DAGING AYAM PEDAGING
ABSTRAK Tetrasiklin merupakan antibiotik yang umum digunakan sebagai obat-obatan veteriner untuk pencegahan penyakit, ada juga yang masuk dalam ransum pakan. Penggunaan yang berlebihan dapat meninggalkan residu dalam produk peternakan tersebut termasuk daging ayam. Implikasi klinis yang dapat terjadi, antara lain alergi, keracunan, dan resistensi. Residu tetrasiklin dalam daging ayam pedaging dapat dianalisis menggunakan spektrofometer ultraviolet dengan teknik adisi standar. Daging ayam pedaging diekstraksi lebih dulu menggunakan metode Matrix Solid Phase Dispersion (MSPD). Hasil uji perolehan kembali berdasarkan metode penambahan standar sebesar 84,40% dengan batas keberterimaan sebesar 80-110%. Penyimpangan baku relatif yang diperoleh 1,36%; batas deteksi dengan konsentrasi 5,16 µg/ml; batas kuantitasi 5,54 µg/ml; dan linieritas 0,9995; 0,9997; dan 0,9998 dari tiga kali ulangan. Hasil uji analisis sampel daging ayam pedaging yang berasal dari tiga pusat perbelanjaan di kota Bandar Lampung menunjukkan kadar residu tetrasiklin 42,40 mg/kg; 61,05 mg/kg; dan 44,47 mg/kg. Nilai tersebut telah melebihi batas maksimum residu tetrasiklin dalam daging menurut SNI 01-6066-2000, yaitu 0,1 mg/kg. Kata kunci : tetrasiklin, daging ayam pedaging, MSPD, spektrofotometri uv ABSTRACT Tetracyline is antibiotic that have been widely used by poultryman to prevent diseases in dairy broiler chicken and sometimes it is added in their ransumes. Overuse of these antibiotic may lead its residue accumulates in animal products including meat. This residue could caused clinic implication such as allergy, intoxication, and spesific of antibiotic resistance. This experiment was aimed to analyze tetracyclines residue in broiler chicken meat with ultraviolet spectrophotometer using standard addition technique. The meat was extracted with Matrix Solid Phase Dispersion (MSPD) method. Recovery was 84.40% according to standard addition method with acceptable limit 80-110%. Relative standar deviation were 1.36%. The limit of detection were 5.16 µg/ml and limit of quantitation were 5.54 µg/ml. The linearity test presented a correlation coefficient were 0.9995; 0.9997; and 0.9998. The analysis of broiler chicken meat from three department store in Bandar Lampung city showed the amount of tetracyclines residue were 42.40 mg/kg; 61.05 mg/kg; and 44.47 mg/kg. These value has exceeded the maximum limit of tetracycline residues in meat according to SNI 016066-2000, i.e. 0.1 mg/kg. Keywords: tetracyclines, broiler chicken meat, MSPD, ultraviolet spectrophotometry
FAKULTAS MATEMATIKA DAN ILMU PENGETAHUAN ALAM, UNIVERSITAS LAMPUNG
VALIDASI METODE MATRIX SOLID PHASE DISPERSION (MSPD)SPEKTROFOTOMETRI UV UNTUK ANALISIS RESIDU TETRASIKLIN DALAM DAGING AYAM PEDAGING (File PDF)
VALIDASI METODE MATRIX SOLID PHASE DISPERSION (MSPD)SPEKTROFOTOMETRI UV UNTUK ANALISIS RESIDU TETRASIKLIN DALAM DAGING AYAM PEDAGING (Other)
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