TY  - THES
ID  - eprints18761
UR  - http://digilib.unila.ac.id/18761/
A1  -  NOFITA , 1327011030
Y1  - 2016/01/12/
N2  - ABSTRAK



Tetrasiklin merupakan antibiotik yang umum digunakan sebagai obat-obatan
veteriner untuk pencegahan penyakit, ada juga yang masuk dalam ransum pakan.
Penggunaan yang berlebihan dapat meninggalkan residu dalam produk peternakan
tersebut termasuk daging ayam. Implikasi klinis yang dapat terjadi, antara lain
alergi, keracunan, dan resistensi. Residu tetrasiklin dalam daging ayam pedaging
dapat dianalisis menggunakan spektrofometer ultraviolet dengan teknik adisi
standar. Daging ayam pedaging diekstraksi lebih dulu menggunakan metode
Matrix Solid Phase Dispersion (MSPD). Hasil uji perolehan kembali berdasarkan
metode penambahan standar sebesar 84,40% dengan batas keberterimaan sebesar
80-110%. Penyimpangan baku relatif yang diperoleh 1,36%; batas deteksi dengan
konsentrasi 5,16 µg/ml; batas kuantitasi 5,54 µg/ml; dan linieritas 0,9995; 0,9997;
dan 0,9998 dari tiga kali ulangan. Hasil uji analisis sampel daging ayam pedaging
yang berasal dari tiga pusat perbelanjaan di kota Bandar Lampung menunjukkan
kadar residu tetrasiklin 42,40 mg/kg; 61,05 mg/kg; dan 44,47 mg/kg. Nilai
tersebut telah melebihi batas maksimum residu tetrasiklin dalam daging menurut
SNI 01-6066-2000, yaitu 0,1 mg/kg.


Kata kunci : tetrasiklin, daging ayam pedaging, MSPD, spektrofotometri uv




ABSTRACT



Tetracyline is antibiotic that have been widely used by poultryman to prevent
diseases in dairy broiler chicken and sometimes it is added in their ransumes.
Overuse of these antibiotic may lead its residue accumulates in animal products
including meat. This residue could caused clinic implication such as allergy,
intoxication, and spesific of antibiotic resistance.  This experiment was aimed to
analyze tetracyclines residue in broiler chicken meat with ultraviolet
spectrophotometer using standard addition technique. The meat was extracted
with Matrix Solid Phase Dispersion (MSPD) method. Recovery was 84.40%
according to standard addition method with acceptable limit 80-110%. Relative
standar deviation were 1.36%. The limit of detection were 5.16 µg/ml and limit of
quantitation were 5.54 µg/ml. The linearity test presented a correlation coefficient
were 0.9995; 0.9997; and 0.9998. The analysis of broiler chicken meat from three
department store in Bandar Lampung city showed  the amount of tetracyclines
residue were 42.40 mg/kg; 61.05 mg/kg; and 44.47 mg/kg. These value has
exceeded the maximum limit of tetracycline residues in meat according to SNI 016066-2000,
i.e.
0.1
mg/kg.





Keywords:

tetracyclines, broiler chicken meat, MSPD, ultraviolet
spectrophotometry 









PB  - UNIVERSITAS LAMPUNG
M1  - masters
TI  - VALIDASI METODE MATRIX SOLID PHASE DISPERSION (MSPD)SPEKTROFOTOMETRI
UV
UNTUK
ANALISIS
RESIDU
TETRASIKLIN

DALAM DAGING AYAM PEDAGING
AV  - restricted
ER  -