?url_ver=Z39.88-2004&rft_val_fmt=info%3Aofi%2Ffmt%3Akev%3Amtx%3Adc&rft.title=DEGRADASI+EKSTRAK+KASAR+KULIT+UDANG+OLEH+Mucor+miehei%0D%0AMENJADI+GLUKOSAMIN+DENGAN+METODE+SPEKTROFOTOMETRI%0D%0AUV-Vis&rft.creator=RUWAIDAH+MULIANA+%2C+1217011052&rft.subject=QD+Chemistry&rft.description=Kitin+merupakan+suatu+polimer+tak+larut+yang+tersusun+dari+residu+%CE%B2-1%2C4-N-asetil-%0D%0AD-glukosamin+(GlcNAc).+Kitin+dapat+diisolasi+dari+kulit+udang+melalui+dua%0D%0Atahapan+proses%2C+yaitu+deproteinasi+dan+demineralisasi.+Selanjutnya+kitin+hasil%0D%0Aisolasi+dapat+didegradasi+dengan+enzim+kitinase+menjadi+monomer-monomer+dan%0D%0Aoligomernya+oleh+Mucor+miehei.+Tujuan+dari+penelitian+ini+adalah+untuk%0D%0Amendapatkan+rendemen+maksimum+dari+ekstrak+kulit+udang+yang+sudah%0D%0Adihilangkan+proteinnya+dengan+cara+deproteinasi.+Fermentasi+selama+2+hari%0D%0Adengan+waktu+pengambilan+sampel+8%2C+16%2C+24%2C+32%2C+40%2C+dan+48+jam.+Glukosamin%0D%0Adalam+rendemen+hasil+fermentasi+direaksikan+menggunakan+senyawa+ninhidrin%0D%0A0%2C8%25+dan+buffer+fosfat+pH+6%2C+akan+membentuk+Ruhemann+purple%0D%0A(diketohidrindamin%E2%80%93diketohidrindiliden)+bila+dipanaskan+pada+temperatur+1000C.%0D%0AAbsorbansi+glukosamin+dan+ninhidrin+diukur+menggunakan+spektrofotometri%0D%0AUV-Vis+pada+%CE%BB+maks+567+nm.+Hasil+pengukuran+diplotkan+ke+dalam+persamaan%0D%0Aregresi+linear+y+%3D+0%2C0094x+%E2%80%93+0%2C1238.+Dari+persamaan+linear+ini+didapatkan%0D%0Ahasilkan+glukosamin+tertinggi+pada+fermentasi+8+jam+yaitu+sebesar+2%2C431%25.%0D%0AKandungan+mineral+yang+masih+ada+pada+glukosamin+seperti+Mg2%2B+dan+Na%2B%0D%0Amengakibatkan+aktivitas+enzim+kitinase+menurun%2C+oleh+sebab+itu+rendemen+yang%0D%0Adihasilkan+sedikit.%0D%0AKata+Kunci+%3A+demineralisasi%2C+deproteinasi%2C+D-gukosamin%2C+kitin%2C+Mucor+miehei.+dan%0D%0Aspektrofotometri+UV-Vis.%0D%0A%0D%0AABSTRACT%0D%0A%0D%0AChitin+is+an+insoluble+polymer+which+composed+by+%CE%B2-1%2C4-N-asetil-D-glucosamine%0D%0A(GlcNAc)+residues.+Chitin+can+be+isolated+from+shirmp+shells+through+two%0D%0Aprocesses%2C+namely+deproteinization+and+demineralization.+Furthermore%2C+chitin+can%0D%0Abe+hidrolyzed+as+its+monomers+and+oligomers+by+chitinase+enzyme+from+Mucor%0D%0Amiehei.+The+aim+of+this+research+is+to+obtain+maximum+yield+from+shrimp+shells%0D%0Aremoved+protein+by+deproteinization+process.+Fermentation+during+2+days%2C+with%0D%0Asampling+every+8%2C+16%2C+24%2C+32%2C+40+and+48+hours.+Glucosamine+from+fermentation%0D%0Areacted+with+0.8%25+ninhydrin+solution+and+phosphate+buffer+pH+6%2C+resulting+in+a%0D%0Aso+called+Ruhemann+purple+color+(diketohydrindamine%E2%80%93diketohydrindylidene)%0D%0Awhen+heated+at+a+temperature+of+1000C.+Glucosamine+and+ninhydrin+absorbance%0D%0Awas+measured+using+UV-Vis+spectrophotometry+a+maximum+absorbance+at+567%0D%0Anm.+The+measurement+results+were+plotted+in+a+linear+regression+equation+y+%3D%0D%0A0%2C0094x+-+0.1238.+The+highest+glucosamine+yields+during+2+days+fermentation+at+8%0D%0Ahours+is+equal+to+2%2C431%25.+Mineral+still+exist+in+glucosamine+such+as+Mg2%2B+and+Na%2B%2C%0D%0Ato+work+on+chitinase+enzyme+activity+declined%2C+therefore+glucosamine+yields%0D%0Agained+slightly.%0D%0AKeywords+%3A+demineralization%2C+deproteinization%2C+D-gucosamine%2C+chitin%2C+Mucor%0D%0Amiehei%2C+and+spectrophotometry+UV-Vis.&rft.publisher=FAKULTAS+MATEMATIKA+DAN+ILMU+PENGETAHUAN+ALAM&rft.date=2016-09-16&rft.type=Skripsi&rft.type=NonPeerReviewed&rft.format=text&rft.identifier=http%3A%2F%2Fdigilib.unila.ac.id%2F24051%2F1%2FABSTRAK%2520%2528ABSTRACT%2529.pdf&rft.format=text&rft.identifier=http%3A%2F%2Fdigilib.unila.ac.id%2F24051%2F2%2FSKRIPSI%2520TANPA%2520BAB%2520PEMBAHASAN.pdf&rft.format=text&rft.identifier=http%3A%2F%2Fdigilib.unila.ac.id%2F24051%2F18%2FSKRIPSI%2520FULL.pdf&rft.identifier=++RUWAIDAH+MULIANA+%2C+1217011052++(2016)+DEGRADASI+EKSTRAK+KASAR+KULIT+UDANG+OLEH+Mucor+miehei+MENJADI+GLUKOSAMIN+DENGAN+METODE+SPEKTROFOTOMETRI+UV-Vis.++FAKULTAS+MATEMATIKA+DAN+ILMU+PENGETAHUAN+ALAM%2C+UNIVERSITAS+LAMPUNG.+++++&rft.relation=http%3A%2F%2Fdigilib.unila.ac.id%2F24051%2F