?url_ver=Z39.88-2004&rft_val_fmt=info%3Aofi%2Ffmt%3Akev%3Amtx%3Adc&rft.title=PENINGKATAN+KESTABILAN+ENZIM+PROTEASE+DARI%0D%0ABacillus+subtilis+ITBCCB148+DENGAN+AMOBILISASI%0D%0AMENGGUNAKAN+ZEOLIT&rft.creator=USWATUN+HASANAH+%2C+1117011050&rft.subject=QD+Chemistry&rft.description=ABSTRAK%0D%0APenelitian+ini+bertujuan+untuk+meningkatkan+stabilitas+enzim+protease+dari%0D%0Aisolat+bakteri+lokal+Bacillus+subtilis+ITBCCB148+dengan+amobilisasi%0D%0Amenggunakan+zeolit.+Untuk+mencapai+tujuan+tersebut+maka+dilakukan+proses%0D%0Aproduksi%2C+isolasi%2C+pemurnian%2C+amobilisasi+enzim%2C+dan+karakterisasi+enzim+protease%0D%0Asebelum+dan+sesudah+amobilisasi.%0D%0AAktivitas+spesifik+enzim+hasil+pemurnian+diperoleh+sebesar+2.680%2C734+U%2Fmg%2C%0D%0Ameningkat+13+kali+dibandingkan+ekstrak+kasar+enzim+yaitu+204%2C465+U%2Fmg.+Enzim%0D%0Ahasil+pemurnian+bekerja+optimum+pada+suhu+50%C2%BAC%2C+sedangkan+enzim+amobil+pada%0D%0Asuhu+55%C2%BAC.+Aktivitas+sisa+yang+dihasilkan+pada+uji+stabilitas+termal+pada+suhu%0D%0A60%C2%BAC+selama+60+menit+terhadap+enzim+hasil+pemurnian+adalah+sebesar+2%2C215%25%2C%0D%0Asedangkan+enzim+amobil+sebesar+16%2C971%25.+Data+kinetika+enzim+hasil+pemurnian%0D%0Adiperoleh+data+KM+%3D+21+mg+substrat+mL-1%2C+Vmaks+%3D+500+%CE%BCmol+mL-1+menit-1%2C+t1%2F2+%3D%0D%0A10%2C661+menit%2C+ki+%3D+0%2C065+menit-1+dan+%CE%94Gi+%3D+97%2C667+kJ+mol-1%2C+sedangkan+enzim%0D%0Aamobil+adalah+KM+%3D+8%2C6+mg+substrat+mL-1%2C+Vmaks+%3D+200+%CE%BCmol+mL-1+menit-1+%2C+t1%2F2+%3D%0D%0A26%2C653+menit%2C+ki+%3D+0%2C026+menit-1+dan+%CE%94Gi+%3D+101%2C685+kJ+mol-1.+Amobilisasi%0D%0Amenggunakan+zeolit+telah+berhasil+meningkatkan+2%2C5+kali+stabilitas+termal+enzim%2C%0D%0Ayang+ditunjukkan+oleh+penurunan+nilai+ki.%0D%0AKata+kunci+%3A+Protease%2C+Bacillus+subtilis+ITBCCB148%2C+amobilisasi+enzim%2C+zeolit.%0D%0A%0D%0AABSTRACT%0D%0A%0D%0AThe+objective+of+this+research+was+to+improve+protease+enzyme+stability%0D%0Afrom+local+bacteria+isolates+of+bacillus+subtilis+ITBCCB148+with+immobilization%0D%0Ausing+zeolite.+A+sequential+processes+were+conducted%2C+i.e%3A+production%2C+isolation%2C%0D%0Apurification%2C+immobilization%2C+and+characterization+of+the+protease+before+and+after%0D%0Aimmobilization.%0D%0AThe+specific+activity+of+purified+enzyme+was+2%2C680.734+U%2Fmg%2C+increased+13%0D%0Atimes+higher+than+the+raw+extract+(204.465+U%2Fmg).+The+purified+enzyme+worked%0D%0Awell+at+50%C2%BAC+and+the+immobilized+at+55%C2%BAC.+From+thermal+stability+test+at+60%C2%BAC+for%0D%0A60+minutes%2C+residual+activity+of+the+purified+and+the+immobilized+enzyme+were%0D%0A2.215%25+and+16.971%25%2C+respectively.+Kinetic+datas+of+the+purified+enzyme+were+KM%0D%0Avalue+%3D+21+mg+substrat+mL-1%2C+Vmaks+%3D+500+%CE%BCmol+mL-1+minute-1%2C+t1%2F2+%3D+10.661%0D%0Amenute%2C+ki+%3D+0.065+minute-1%2C+and+%CE%94Gi+%3D+97.667+kJ+mol-1%2C+while+the+immobilized%0D%0Awere+KM+%3D+8.6+mg+substrat+mL-1%2C+Vmaks+%3D+200+%CE%BCmol+mL-1+minute-1%2C+t1%2F2+%3D+26.653%0D%0Aminute%2C+ki+%3D+0.026+minute-1+dan+%CE%94Gi+%3D+101.685+kJ+mol-1.+Enzyme+immobilization%0D%0Ausing+zeolite+has+succeeded+in+increasing+the+thermal+stability+of+the+enzyme+as%0D%0Amuch+as+2.5+times%2C+which+is+indicated+be+decrease+in+the+value+of+ki.%0D%0AKeywords+%3A+Protease%2C+Bacillus+subtilis+ITBCCB148%2C+enzyme+immobilization%2C%0D%0Azeolite.%0D%0A%0D%0A%0D%0A&rft.publisher=FAKULTAS+MATEMATIKA+DAN+ILMU+PENGETAHUAN+ALAM&rft.date=2016-10-12&rft.type=Skripsi&rft.type=NonPeerReviewed&rft.format=text&rft.identifier=http%3A%2F%2Fdigilib.unila.ac.id%2F24721%2F1%2FABSTRAK%2520%2528ABSTRACT%2529.pdf&rft.format=text&rft.identifier=http%3A%2F%2Fdigilib.unila.ac.id%2F24721%2F2%2FSKRIPSI%2520TANPA%2520BAB%2520PEMBAHASAN.pdf&rft.format=text&rft.identifier=http%3A%2F%2Fdigilib.unila.ac.id%2F24721%2F3%2FSKRIPSI%2520FULL.pdf&rft.identifier=++USWATUN+HASANAH+%2C+1117011050++(2016)+PENINGKATAN+KESTABILAN+ENZIM+PROTEASE+DARI+Bacillus+subtilis+ITBCCB148+DENGAN+AMOBILISASI+MENGGUNAKAN+ZEOLIT.++FAKULTAS+MATEMATIKA+DAN+ILMU+PENGETAHUAN+ALAM%2C+UNIVERSITAS+LAMPUNG.+++++&rft.relation=http%3A%2F%2Fdigilib.unila.ac.id%2F24721%2F