TY  - GEN
CY  - UNIVERSITAS LAMPUNG
ID  - eprints24771
UR  - http://digilib.unila.ac.id/24771/
A1  - ANA FEBRIANTI WULANDARI,  1117011002
Y1  - 2016/10/12/
N2  - Protease banyak digunakan secara komersial dalam industri pangan dan non-pangan.
Agar dapat digunakan dalam proses industri, maka enzim harus dapat bekerja pada
pH dan suhu ekstrim. Penelitian ini bertujuan untuk meningkatkan stabilitas enzim
protease dari isolat bakteri Bacillus subtilis ITBCCB148 melalui proses amobilisasi
dengan metode penyerapan fisik (adsorpsi) enzim menggunakan bentonit. Adapun
tahapan yang dilakukan dalam penelitian ini, meliputi proses produksi, isolasi,
pemurnian dan amobilisasi enzim hasil pemurnian. Hasil penelitian menunjukkan
aktivitas spesifik enzim hasil pemurnian sebesar 1528,87 U/mg, meningkat
kemurniannya 13,04 kali dibandingkan ekstrak kasar enzim. Enzim protease
memiliki suhu optimum 50ºC, sedangkan enzim amobil pada suhu 55ºC. Uji
stabilitas termal pada suhu 60ºC selama 60 menit untuk enzim hasil pemurnian
masih memiliki aktivitas sisa 2,694%, sedangkan enzim amobil sebesar 17,599%.
Data kinetika enzim hasil pemurnian diperoleh KM = 6,200 mg mL-1 substrat dan
Vmaks = 200 ?mol mL-1 menit-1, t1/2 = 12,6 menit, ki = 0,055 menit-1 dan ?Gi =
98,115 KJ mol-1. Sedangkan data kinetika enzim hasil amobilisasi diperoleh KM =
4,285 mg mL-1 substrat dan Vmaks = 142,857 ?mol mL-1 menit-1, t1/2 = 23,1 menit, ki
= 0,03 menit-1 dan ?Gi = 101,295 KJ mol-1. Berdasarkan penurunan nilai ki ,
peningkatan waktu paruh (t1/2), dan nilai ?Gi, menunjukkan bahwa amobilisasi
menggunakan bentonit dapat meningkatkan stabilitas enzim protease dari Bacillus
subtilis ITBCCB148.
Kata kunci : Protease, Bacillus subtilis ITBCCB148, Amobilisasi enzim, Bentonit

ABSTRACT

Protease is widely used commercially in food and non-food industry. For a certain
industrial processes, the enzyme must be set up at extreme level of both pH and
temperature. This research was aimed to improve the stability of protease from
Bacillus Subtilis ITBCCB148 to immobilization techniques by adsorption of the
enzyme on bentonite. Some sequential steps in this research were including
production, isolation, purification and immobilization of purified enzymes. The
results showed a specific activity of the purified enzyme of 1528,87 U/mg,
increased of 13.04 folds than the crude extract. The purified protease has an
optimum temperature at 50ºC, whereas the immobilized enyme at 55ºC. the
residual activity on 60°C for 60 minutes for purified enzyme was 2.694%, while
the immobilized enzyme was 17.599%. Kinetic datas of purified enzyme results
were KM = 6.200 mg mL-1 substrate, Vmax = 200 ?mol mL-1 minute-1, t1/2 = 12.6
minutes, ki = 0.055 min-1 and ?Gi = 98.115 KJ mol-1. While the datas of the
immobilized enzyme were KM = 4.285 mg mL-1 substrate, Vmax = 142.857 ?mol
mL-1 minute-1, t1/2 = 23.1 minutes, ki = 0.03 minute-1 and ?Gi = 101.295 KJ mol-1.
Based on impairment of ki, increment in half-time (t1/2), and the value of ?Gi, the
immobilization by bentonite can improve the stability of protease from Bacillus
subtilis ITBCCB148.
Key words: Protease, Bacillus subtilis ITBCCB148, Enzyme immobilization,
Bentonite
PB  - FAKULTAS MATEMATIKA DAN ILMU PENGETAHUAN ALAM
TI  - AMOBILISASI ENZIM PROTEASE DARI
Bacillus subtilis ITBCCB148 MENGGUNAKAN BENTONIT
AV  - restricted
ER  -