?url_ver=Z39.88-2004&rft_val_fmt=info%3Aofi%2Ffmt%3Akev%3Amtx%3Adc&rft.title=AMOBILISASI+ENZIM+%CE%B1-AMILASE+DARI+Bacillus+subtilis+ITBCCB148+%0D%0AMENGGUNAKAN+BENTONIT+%0D%0A&rft.creator=Ezra+Rheinsky+Tiarsa%2C+1317011019&rft.subject=Q+Science+(General)&rft.description=ABSTRACT%0D%0A%0D%0AIn+this+study%2C+the+%CE%B1-amylase+from+Bacillus+subtilis+ITBCCB148+was+immobilized+by+using+bentonite.++The+aims+of+this+research+were+to+increase+the+stability+of++++++%CE%B1-amylase+and+to+help+in+their+economic+reuse.++The+steps+of+this+research+were+done+as+following%3A+production%2C+isolation%2C+purification%2C+immobilization%2C+and+characterization+of+purified+and+immobilized+enzymes.+The+result+showed+that+the+purified+enzyme+from+CMC+cation+column+chromatography+process+has+specific+activity+was+10387.11+U+mg-1.++It+was+higher+12+times+than+the+crude+extract.++The+optimum+temperature+of+purified+enzyme+was+60oC%2C+KM+%3D+6.18+mg+mL-1+substrate%2C+and+Vmax+%3D+909.09+%C2%B5mol+mL-1+min-1.++The+optimum+temperature+of+immobilized+enzyme+was+75oC%2C+KM+%3D+12.19+mg+mL-1+substrate%2C+and+Vmax+%3D+88.50+%C2%B5mol+mL-1+min-1.++Residual+activities+of+purified+and+immobilized+enzyme+in+the+study+of+thermal+stability+on+60oC+for+100+minutes+approximately+were+12%25+and+43%25.++The+activity+of+immobilized+enzyme+was+maintained+significantly+even+after+5+reuses.++The+kinetic+studies+of+purified+enzyme+were+obtained+t%C2%BD+%3D+42.00+minutes%2C+ki+%3D+0.0165+min-1%2C+and+%CE%94Gi+%3D+104.57+kJ+mol-1.++The+kinetic+studies+of+immobilized+enzyme+were+obtained+t%C2%BD+%3D+88.85+minutes%2C+ki+%3D+0.0078+min-1%2C+and+%CE%94Gi+%3D+106.65+kJ+mol-1.++Based+on+the+increase+of+half-time+(t%C2%BD)%2C+decrease+of+ki%2C+and+increase+of+%CE%94Gi%2C+the+immobilization+by+using+bentonite+can+improve+the+stability+of+%CE%B1-amylase+from+B.+subtilis+ITBCCB148.%0D%0A%0D%0A%0D%0AKeywords%3A+%CE%B1-amylase%2C+Bacillus+subtilis+ITBCCB148%2C+immobilization%2C+bentonite%0D%0A%0D%0A%0D%0AABSTRAK%0D%0A%0D%0APada+penelitian+ini+telah+dilakukan+amobilisasi+enzim+%CE%B1-amilase+dari+Bacillus+subtilis+ITBCCB148+menggunakan+matriks+bentonit.++Penelitian+ini+bertujuan+untuk+meningkatkan+kestabilan+enzim+dan+agar+enzim+dapat+digunakan+secara+berulang.++Tahap+penelitian+ini+meliputi+proses+produksi%2C+isolasi%2C+pemurnian%2C+amobilisasi%2C+dan+karakterisasi+enzim+murni+dan+amobil.++Hasil+penelitian+menunjukkan+bahwa+aktivitas+spesifik+enzim+%CE%B1-amilase+hasil+pemurnian+hingga+tahap+kromatografi+penukar+kation+menggunakan+CMC+adalah+10387%2C11+U+mg-1+dan+kemurniannya+meningkat+12+kali+dibandingkan+dengan+ekstrak+kasar+enzim.++Enzim+%CE%B1-amilase+hasil+pemurnian+memiliki+suhu+optimum+60oC%2C+KM+%3D+6%2C18+mg+mL-1+substrat%2C+dan+Vmaks+%3D+909%2C09+%C2%B5mol+mL-1+menit-1.++Enzim+%CE%B1-amilase+hasil+amobilisasi+memiliki+suhu+optimum+75oC%2C+KM+%3D+12%2C19+mg+mL-1+substrat%2C+dan+Vmaks+%3D+88%2C50+%C2%B5mol+mL-1+menit-1.++Aktivitas+sisa+enzim+murni+dan++enzim+amobil+dalam+uji+stabilitas+termal+pada+suhu+60oC+selama+100+menit+berturut-turut+sebesar+12%25+dan+43%25.++Enzim+amobil+dapat+digunakan+berulang+hingga+5+kali.++Data+kinetika+enzim+hasil+pemurnian+diperoleh+t%C2%BD+%3D+42%2C00+menit%2C+ki+%3D+0%2C0165+menit-1%2C+dan+%CE%94Gi+%3D+104%2C57+kJ+mol-1.++Data+kinetika+enzim+hasil+amobilisasi+diperoleh+t%C2%BD+%3D+88%2C85+menit%2C+ki+%3D+0%2C0078+menit-1%2C+dan+%CE%94Gi+yaitu+106%2C65+kJ+mol-1.++Berdasarkan+penurunan+nilai+ki%2C+peningkatan+nilai+%CE%94Gi+dan+waktu+paruh+(t%C2%BD)%2C+diketahui+bahwa+amobilisasi+menggunakan+bentonit+dapat+meningkatkan+stabilitas+enzim+%CE%B1-amilase+dari+B.+subtilis+ITBCCB148.%0D%0A%0D%0A%0D%0AKata+kunci%3A+%CE%B1-amilase%2C+Bacillus+subtilis+ITBCCB148%2C+amobilisasi%2C+bentonit%0D%0A&rft.publisher=FAKULTAS+MATEMATIKA+DAN+ILMU+PENGETAHUAN+ALAM&rft.date=2017-07-10&rft.type=Skripsi&rft.type=NonPeerReviewed&rft.format=text&rft.identifier=http%3A%2F%2Fdigilib.unila.ac.id%2F27360%2F1%2FABSTRAK%2520%2528ABSTRACT%2529.pdf&rft.format=text&rft.identifier=http%3A%2F%2Fdigilib.unila.ac.id%2F27360%2F2%2FSKRIPSI%2520FULL.pdf&rft.format=text&rft.identifier=http%3A%2F%2Fdigilib.unila.ac.id%2F27360%2F3%2FSKRIPSI%2520TANPA%2520BAB%2520PEMBAHASAN.pdf&rft.identifier=++Ezra+Rheinsky+Tiarsa%2C+1317011019++(2017)+AMOBILISASI+ENZIM+%CE%B1-AMILASE+DARI+Bacillus+subtilis+ITBCCB148+MENGGUNAKAN+BENTONIT.++FAKULTAS+MATEMATIKA+DAN+ILMU+PENGETAHUAN+ALAM%2C+UNIVERSITAS+LAMPUNG.+++++&rft.relation=http%3A%2F%2Fdigilib.unila.ac.id%2F27360%2F