?url_ver=Z39.88-2004&rft_val_fmt=info%3Aofi%2Ffmt%3Akev%3Amtx%3Adc&rft.title=MODIFIKASI+KIMIA+ENZIM+PROTEASE+DARI+Rhizopusoligosporus+MENGGUNAKAN+SITRAKONAT+ANHIDRIDA&rft.creator=Khomsatun+Khasanah%2C+1317011034&rft.subject=Q+Science+(General)&rft.subject=QD+Chemistry&rft.description=ABSTRACT%0D%0A%0D%0AIn+this+research%2C+enzyme+chemical+modification+by+citraconic+anhydride+had+been+done+to+increase+the+stability+of+protease+that+was+produced+by+Rhizopusoligosporus.+This+research+steps+had+been+done+as+followsproduction+and+isolation%2C+purification%2C+chemical+modification+by+usingcitraconic+anhydride%2C+and+characterization.++The+result+showed+that+the+purified+enzyme+has+specific+activity+70.754U+mg-1%2C+increased+7+times+compare+with+the+crude+extract+enzyme.++The+purified+enzyme+hadoptimum+pH+andtemperature+approximately+were+6.5+and45%C2%B0C%3B+KM+%3D+2.853+mg+mL-1+substrate%3B+Vmax+%3D+1.907+%C2%B5mol+mL-1minutes-1%3B+ki+%3D+0.0436+minutes-1%3B+t1%2F2+%3D+15.89+minutes+and+%E2%88%86Gi%3D+97.17+kJ+mol-1.++The+modified+enzymes+bycitraconic+anhydride+20+%CE%BCL%2C+30+%CE%BCL%2C+and+40+%CE%BCLhadoptimum+pHand+temperatureapproximately+were+7.0+and+55%C2%BAC%3BKMrow+as+follows+%3A+3.020%3B+2.679%3B+2.923+mg+mL-1+substrate%3B+Vmax+row+as+follows%3A+1.852%3B+1.618+%3B1.033+%C2%B5mol+mL-1minutes-1%3B+ki+row+as+follows%3A+0.0188+%3B+0.0182+%3B+0.0135+minutes-1%3B+half+time+(t1%2F2)+row+as+follows%3A+36.86+%3B+38.08+%3B+51.33+minutes+and+%E2%88%86Girows+as+follows+%3A+102.604+%3B+102.693+and+103.508+kJ++mol-1.++Based+on+the+decrease+of+ki+values%2C+increase+of+t1%2F2+and+%E2%88%86Gi%2C+were+known+that+chemical+modification+by+citraconic+anhydride+could+increase+the+stability+of+protease+from+Rhizopusoligosporus.%0D%0A%0D%0A%0D%0A%0D%0AKeywords+%3ARhizopusoligosporus%2C+protease+enzyme%2C+chemical+modification%2C%0D%0Acitraconic+anhydride%0D%0A%0D%0A%0D%0AABSTRAK%0D%0A%0D%0APada+penelitian+ini+telah+dilakukan+modifikasi+kimia+enzim+protease+dari+Rhizopus%0D%0Aoligosporus+menggunakan+sitrakonat+anhidrida+untuk+meningkatkan+stabilitas%0D%0Aenzim+tersebut.+Penelitian+ini+dilakukan+dengan+beberapa+tahap%2C+yaitu+%3A+produksi%0D%0Adan+isolasi%2C+pemurnian%2C+modifikasi+kimia+menggunakan+sitrakonat+anhidrida%2C+dan%0D%0Akarakterisasi.+Hasil+penelitian+menunjukkan+bahwa+aktivitas+spesifik+enzim%0D%0Aprotease+hasil+pemurnian+70%2C754+U+mg-1%2C+meningkat+7+kali+dibandingkan+dengan%0D%0Aekstrak+kasar+enzim.+Enzim+hasil+pemurnian+ini+mempunyai+pH+optimum+6%2C5%3B+suhu%0D%0Aoptimum+45%C2%B0C%3B+KM+%3D+2%2C853+mg+mL-1+substrat%3B+Vmaks+%3D+1%2C907+%CE%BCmol+mL-1+menit-1%3B+ki%0D%0A%3D+0%2C0436+menit-1%3B+t1%2F2+%3D+15%2C89+menit+dan+%CE%94Gi+%3D+97%2C17+kJ+mol-1.+Enzim+hasil%0D%0Amodifikasi+menggunakan+sitrakonat+anhidrida+dengan+variasi+penambahan+20+%CE%BCL%2C%0D%0A30+%CE%BCL%2C+dan+40+%CE%BCL+mempunyai+pH+optimum+7%2C0%3B+suhu+55%C2%BAC%3B+KM+berturut-turut%0D%0Asebagai+berikut+%3A+3%2C020+%3B+2%2C679+%3B+2%2C923+mg+mL-1+substrat%3B+Vmaks+berturut-turut+sebagai%0D%0Aberikut+%3A+1%2C852+%3B+1%2C618+%3B+1%2C033+%CE%BCmol+mL-1+menit-1%3B+ki+berturut-turut+sebagai+berikut+%3A%0D%0A0%2C0188+%3B+0%2C0182+dan+0%2C0135+menit-1+%3B+waktu+paruh+berturut-turut+sebagai+berikut+%3A%0D%0A36%2C86+%3B+38%2C08+%3B+51%2C33+menit+dan+%CE%94Gi+berturut-turut+%3A+102%2C604+%3B+102%2C693+dan+103%2C508%0D%0AkJ+mol-1.+Berdasarkan+penurunan+nilai+.+ki%2C+peningkatan+waktu+paruh+(t1%2F2)+dan+nilai%0D%0A%CE%94Gi%2C+diketahui+bahwa+modifikasi+kimia+menggunakan+sitrakonat+anhidrida+dapat%0D%0Ameningkatkan+stabilitas+enzim+protease+dari+Rhizopus+oligosporus.%0D%0AKata+kunci+%3A+Rhizopus+oligosporus%2C+Enzim+protease%2C+Modifikasi+kimia%2C+Sitrakonat%0D%0Aanhidrida&rft.publisher=FAKULTAS+MATEMATIKA+DAN+ILMU+PENGETAHUAN+ALAM&rft.date=2017-07-27&rft.type=Skripsi&rft.type=NonPeerReviewed&rft.format=text&rft.identifier=http%3A%2F%2Fdigilib.unila.ac.id%2F27847%2F1%2FABSTRACT%2520%2528ABSTRAK%2529.pdf&rft.format=text&rft.identifier=http%3A%2F%2Fdigilib.unila.ac.id%2F27847%2F3%2FSKRIPSI%2520FULL.pdf&rft.format=text&rft.identifier=http%3A%2F%2Fdigilib.unila.ac.id%2F27847%2F2%2FSKRIPSI%2520TANPA%2520BAB%2520PEMBAHASAN.pdf&rft.identifier=++Khomsatun+Khasanah%2C+1317011034++(2017)+MODIFIKASI+KIMIA+ENZIM+PROTEASE+DARI+Rhizopusoligosporus+MENGGUNAKAN+SITRAKONAT+ANHIDRIDA.++FAKULTAS+MATEMATIKA+DAN+ILMU+PENGETAHUAN+ALAM%2C+UNIVERSITAS+LAMPUNG.+++++&rft.relation=http%3A%2F%2Fdigilib.unila.ac.id%2F27847%2F