?url_ver=Z39.88-2004&rft_val_fmt=info%3Aofi%2Ffmt%3Akev%3Amtx%3Adc&rft.title=AMOBILISASI+ENZIM+%CE%B1-AMILASE++DARI+Bacillus+subtilis+ITBCCB148+DENGAN+MENGGUNAKAN+ZEOLIT&rft.creator=Fathaniah+Sejati%2C++1317011021&rft.subject=Q+Science+(General)&rft.description=Enzim+%CE%B1-amilase+merupakan+enzim+yang+dapat+memutus+ikatan+%CE%B1-1%2C4+glikosida+pada+amilum.++Enzim+ini+banyak+dimanfaatkan+dalam+berbagai+proses+industri%2C+baik+industri+pangan+maupun+non-pangan.++Dalam+proses+industri+enzim+harus+mampu+bekerja+pada+kondisi+pH+ekstrim+serta+mempunyai+stabilitas+termal+yang+tinggi.++Namun%2C+umumnya+enzim+tidak+stabil+pada+kondisi+tersebut.%0D%0APenelitian+ini+bertujuan+untuk+meningkatkan+stabilitas+enzim+%CE%B1-amilase+dari+isolat+bakteri+lokal+Bacillus+subtilis+ITBCCB148+dengan+amobilisasi+menggunakan+zeolit.++Untuk+mencapai+tujuan+tersebut+maka+dilakukan+proses+produksi%2C+isolasi%2C+pemurnian%2C+amobilisasi+enzim%2C+dan+karakterisasi+enzim+%CE%B1-amilase+sebelum+dan+sesudah+amobilisasi.%0D%0AAktivitas+spesifik+enzim+hasil+pemurnian+diperoleh+sebesar+24.735%2C715+U%2Fmg%2C+meningkat+19+kali+dibandingkan+ekstrak+kasar+enzim+yaitu+1.285%2C867+U%2Fmg.+Enzim+hasil+pemurnian+bekerja+optimum+pada+suhu+65%C2%BAC%2C+sedangkan+enzim+amobil+pada+suhu+75%C2%BAC.+Aktivitas+sisa+yang+dihasilkan+pada+uji+stabilitas+termal+pada+suhu+65%C2%BAC+selama+100+menit+terhadap+enzim+hasil+pemurnian+adalah+sebesar+20%25%2C++sedangkan+enzim+amobil+sebesar+40%25.++Data+kinetika+enzim+hasil+pemurnian+diperoleh+data+KM+%3D+7%2C543+mg+mL+1%2C+Vmaks+%3D+147%2C058++%CE%BCmol+mL-1++menit-1%2C+t1%2F2+%3D+30+menit%2C+ki+%3D+0%2C023+menit-1+dan+%CE%94Gi+%3D+103%2C65+kJ+mol-1%2C+sedangkan+enzim+amobil+adalah+KM+%3D+6%2C779+mg+mL-1%2C+Vmaks+%3D+97%2C087+%CE%BCmol+mL-1+menit-1+%2C+t1%2F2+%3D+49+menit%2C+ki+%3D+0%2C014+menit-1+dan+%CE%94Gi+%3D+105%2C03+kJ+mol-1.++Amobilisasi+menggunakan+zeolit+telah+berhasil+meningkatkan+1%2C64+kali+stabilitas+termal+enzim%2C+yang+ditunjukkan+oleh+penurunan+nilai+ki.%0D%0AKata+kunci+%3A+%CE%B1-Amilase%2C+Bacillus+subtilis+ITBCCB148%2C+amobilisasi+enzim%2C+zeolit.%0D%0A%0D%0A%0D%0AABSTRACT%0D%0A%0D%0A%0D%0A%CE%B1-amylase+is+an+enzyme+that+breaks+%CE%B1-1-4+glycoside+bond+in+amylum.++It+has+been+widely+used+in+a+number+of+industrial+processes+such+as+food+industry+and+non+food+industry.++In+industrial+process%2C+this+enzyme+must+be+able+to+work+in+an+extreme+pH+and+temperature.++However%2C+an+enzyme+is+not+normally+stable+in+these+conditions.%0D%0AThe+objective+of+this+research+was+to+improve+the+stability+of+%CE%B1-amylase+enzyme+from+local+bacteria+bacillus+subtilis+ITBCCB148+with+immobilization+using+zeolite.++A+sequential+processes+were+conducted%2C+such+as+by+production%2C+isolation%2C+purification%2C+immobilization%2C+and+characterization+the+%CE%B1-amylase+before+and+after+immobilization.%0D%0AThe+specific+activity++of+purified+enzyme+was+obtained+24735.715+U%2Fmg%2C+increased+19+times+higher+than+the+crude+extract+enzyme+(1285.867+U%2Fmg).++The+purified+enzyme+worked+well+at+65%C2%BAC+and+the+immobilized+at+75%C2%BAC.+From+thermal+stability+test+at+65%C2%BAC+for+100+minutes%2C+residual+activity+of+the+purified+and+the+immobilized+enzyme+were+20%25+and+40%25%2C+respectively.++Kinetic+data+of+the+purified+enzyme+were+KM+value+%3D+7.543+mg+mL+1%2C+Vmaks+%3D+147.058+%CE%BCmol+mL-1+minute-1%2C+t1%2F2+%3D+30+minutes%2C+ki+%3D+0.023+minute-1%2C+and+%CE%94Gi+%3D+103.65+kJ+mol-1%2C+while+the+immobilized+were+KM+%3D+6.779+mg+mL-1%2C+Vmaks+%3D+97.087+%CE%BCmol+mL-1+minute-1%2C+t1%2F2+%3D+49+minutes%2C+ki+%3D+0.014+minute-1+dan+%CE%94Gi+%3D+105.03+kJ+mol-1.+Enzyme+immobilization+using+zeolite+has+succeeded+in+increasing+the+thermal+stability+of+the+enzyme+as+much+as+1.64+times%2C+which+is+indicated+by+decrease+of+ki+value.%0D%0AKeywords+%3A+%CE%B1-amylase%2C+Bacillus+subtilis+ITBCCB148%2C+enzyme+immobilization%2C+zeolite.%0D%0A&rft.publisher=FAKULTAS+MATEMATIKA+DAN+ILMU+PENGETAHUAN+ALAM&rft.date=2017-07-26&rft.type=Skripsi&rft.type=NonPeerReviewed&rft.format=text&rft.identifier=http%3A%2F%2Fdigilib.unila.ac.id%2F27851%2F1%2FABSTRAK.pdf&rft.format=text&rft.identifier=http%3A%2F%2Fdigilib.unila.ac.id%2F27851%2F2%2FSKRIPSI%2520FULL.pdf&rft.format=text&rft.identifier=http%3A%2F%2Fdigilib.unila.ac.id%2F27851%2F3%2FSKRPSI%2520TANPA%2520BAB%2520PEMBAHASAN.pdf&rft.identifier=++Fathaniah+Sejati%2C+1317011021++(2017)+AMOBILISASI+ENZIM+%CE%B1-AMILASE+DARI+Bacillus+subtilis+ITBCCB148+DENGAN+MENGGUNAKAN+ZEOLIT.++FAKULTAS+MATEMATIKA+DAN+ILMU+PENGETAHUAN+ALAM%2C+UNIVERSITAS+LAMPUNG.+++++&rft.relation=http%3A%2F%2Fdigilib.unila.ac.id%2F27851%2F