%A 1314111018 Diah Permatasari %T Aplikasi Bacillus sp. D2.2 dalam Sinbiotik Terhadap Respon Imun Seluler Udang Vaname ( Litopenaeus vannamei ) %X ABSTRACT Application of Bacillus sp. D2.2 in Sinbiotics Against Cellular Immune Response White Shrimp ( Litopenaeus vannamei ) By Diah Permatasari Bacillus sp. D2.2 is a probiotic that can be used in an effort to improve the cellular immune system of vaname shrimp. Bacillus sp. D2.2 in sinbiotics added to commercial feed with FR 3% is an alternative combination of bacterial treatment in vaname shrimp. Application of the addition of Bacillus sp. D2.2 in sinbiotics on commercial feed is aimed to determine the effect of probiotics given to cellular immune response of van ame shrimp. Shrimp vaname used 12 gram size as much as 10 each tail tub. The test tubes used were 4 fruits for treatment A (0% probiotics), B (2% probiotics), C (4% probiotics), and D (6% probiotics) added 3% white sweet potato flour extract and binder Egg s 2%). The study was conducted for 12 days of maintenance period with total test parameters of hemocyte count, phagocytosis activity, phagocytosis index. Bacillus sp. D2.2 the applied has an effect on the increase in total hemocyte count and phagocytosis a ctivity, as well as the phagocytosis index. The best percentage of probiotics in improving cellular immune response of vanilla shrimp is Bacillus sp. D2.2 treatment D with 6% concentration as evidenced by increasing THC value and phagocytic activity of van ame shrimp in observation, whereas phagocytosis index value owned by treatment D is stable index compared to other treatment. Keywords: probiotics, Bacillus sp. D2.2, THC, phagocytosis activity, phagocytosis index ABSTRAK Aplikasi Bacillus sp. D2.2 dalam Sinbiotik Terhadap Respon Imun Seluler Udang Vaname ( Litopenaeus vannamei ) Oleh Diah Permatasari Bacillus sp. D2.2 merupakan probiotik yang dapat digunakan dalam upaya peningkatan sistem imun seluler udang vaname. Iso lat Bacillus sp. D2.2 dalam sinbiotik yang ditambahkan pada pakan komersil dengan FR 3% merupakan perpaduan alternatif pengobatan bakterial pada udang vaname. Aplikasi penambahan Bacillus sp. D2.2 dalam sinbiotik pada pakan komersil ditujukan untuk mengeta hui pengaruh probiotik yang diberikan terhadap respon imun seluler udang vaname. Udang vaname yang digunakan berukuran 12 gram sebanyak 10 ekor tiap bak. Bak uji yang digunakan berjumlah 4 buah untuk perlakuan A (0% probiotik), B (2% probiotik), C (4% prob iotik), dan D (6% probiotik) yang ditambahkan ekstrak tepung ubi jalar putih 3% dan binder (putih telur 2%). Penelitian dilakukan selama 12 hari masa pemeliharaan dengan parameter uji total hemocyte count , aktivitas fagositosis, indeks fagositosis. Bacillu s sp. D2.2 yang diaplikasikan memiliki pengaruh terhadap peningkatan total hemocyte count d an aktivitas fagositosis, serta indeks fagositosis. Persentase probiotik terbaik dalam meningkatkan respon imun seluler udang vaname yakni Bacillus sp. D2.2 perlakuan D dengan konsentrasi 6% yang dibuktikan dengan meningkatnya nilai THC dan aktivitas fagositosis udang vaname dalam pengamatan, sedangkan nilai indeks fagositosis yang dimiliki oleh perlakuan D merupakan indeks yang stabil dibandingkan perlakuan l ainnya. Kata kunci : probiotik, Bacillus sp. D2.2, THC, aktivitas fagositosis, indeks fagositosis %C UNIVERSITAS LAMPUNG %D 2017 %I FAKULTAS PERTANIAN %L eprints28161