%A 1314111018 Diah Permatasari
%T Aplikasi 
Bacillus
sp. D2.2 dalam Sinbiotik Terhadap Respon Imun Seluler 
Udang Vaname (
Litopenaeus vannamei
)
%X 
ABSTRACT
Application of Bacillus sp. D2.2 in Sinbiotics Against
Cellular Immune 
Response White Shrimp (
Litopenaeus vannamei
)
By
Diah Permatasari
Bacillus
sp. D2.2 is a probiotic that can be used in an effort to improve the cellular 
immune  system  of  vaname  shrimp. 
Bacillus
sp.  D2.2  in  sinbiotics  added  to 
commercial 
feed with FR 3% is an alternative combination of bacterial treatment 
in vaname shrimp. Application of the addition of 
Bacillus
sp. D2.2 in sinbiotics on 
commercial  feed  is  aimed  to  determine  the  effect  of  probiotics  given  to  cellular 
immune response of van
ame shrimp. Shrimp vaname used 12 gram size as much 
as  10  each  tail  tub.  The  test  tubes  used  were  4  fruits  for  treatment  A  (0% 
probiotics),  B  (2%  probiotics),  C  (4%  probiotics),  and  D  (6%  probiotics)  added 
3%  white  sweet  potato  flour  extract  and  binder  Egg
s  2%).  The  study  was 
conducted  for  12  days  of  maintenance  period  with  total  test  parameters  of 
hemocyte  count,  phagocytosis  activity,  phagocytosis  index. 
Bacillus
sp. D2.2  the 
applied  has  an  effect  on  the  increase  in  total  hemocyte  count  and  phagocytosis 
a
ctivity,  as  well  as  the  phagocytosis  index.  The  best  percentage  of  probiotics  in 
improving  cellular  immune  response  of  vanilla  shrimp  is 
Bacillus
sp.  D2.2 
treatment  D  with  6%  concentration  as  evidenced  by  increasing  THC  value  and 
phagocytic activity of van
ame shrimp in observation, whereas phagocytosis index 
value owned by treatment D is stable index compared to other treatment. 
Keywords:
probiotics, 
Bacillus
sp.    D2.2,    THC,    phagocytosis    activity, 
phagocytosis index
ABSTRAK
Aplikasi 
Bacillus
sp. D2.2 dalam Sinbiotik Terhadap Respon Imun Seluler 
Udang Vaname (
Litopenaeus vannamei
)
Oleh
Diah Permatasari
Bacillus 
sp.  D2.2  merupakan  probiotik  yang  dapat  digunakan  dalam  upaya 
peningkatan  sistem  imun  seluler  udang  vaname.    Iso
lat 
Bacillus 
sp.  D2.2  dalam 
sinbiotik  yang  ditambahkan  pada  pakan  komersil  dengan  FR  3%  merupakan 
perpaduan   alternatif   pengobatan   bakterial   pada   udang   vaname.    Aplikasi 
penambahan 
Bacillus 
sp.  D2.2  dalam  sinbiotik  pada  pakan  komersil  ditujukan 
untuk  mengeta
hui  pengaruh  probiotik  yang  diberikan  terhadap  respon  imun 
seluler  udang  vaname.  Udang  vaname   yang   digunakan  berukuran  12  gram 
sebanyak  10  ekor  tiap  bak.  Bak  uji  yang  digunakan  berjumlah  4  buah  untuk 
perlakuan  A  (0%  probiotik),  B  (2%  probiotik),  C  (4%  prob
iotik),  dan  D  (6% 
probiotik)  yang ditambahkan ekstrak tepung ubi jalar putih 3% dan binder (putih 
telur   2%).   Penelitian   dilakukan   selama   12   hari   masa   pemeliharaan   dengan 
parameter  uji  total 
hemocyte  count
,  aktivitas  fagositosis,  indeks  fagositosis. 
Bacillu
s 
sp.  D2.2  yang  diaplikasikan  memiliki  pengaruh  terhadap  peningkatan 
total 
hemocyte count d
an aktivitas fagositosis, serta indeks fagositosis. Persentase 
probiotik  terbaik  dalam  meningkatkan  respon  imun  seluler  udang  vaname  yakni 
Bacillus 
sp.  D2.2 
perlakuan  D  dengan  konsentrasi  6%  yang  dibuktikan  dengan 
meningkatnya   nilai   THC   dan   aktivitas   fagositosis   udang   vaname   dalam 
pengamatan,  sedangkan  nilai  indeks  fagositosis  yang  dimiliki  oleh  perlakuan  D 
merupakan indeks yang stabil dibandingkan perlakuan l
ainnya.
Kata  kunci
:  probiotik,  Bacillus  sp.  D2.2,  THC,  aktivitas  fagositosis,  indeks 
fagositosis

%C UNIVERSITAS LAMPUNG
%D 2017
%I FAKULTAS PERTANIAN
%L eprints28161