%A 1217011025 Fifi Adriyanthi
%T PENGARUH PENAMBAHAN SORBITOL TERHADAP STABILITAS
ENZIM SELULASE DARI Rhizopus oligosporus
%X Penelitian ini bertujuan untuk mempelajari pengaruh penambahan sorbitol
terhadap kestabilan enzim selulase dari Rhizopus oligosporus menggunakan
senyawa poliol yaitu sorbitol. Adapun tahapan yang dilakukan dalam penelitian
ini meliputi : produksi, isolasi, pemurnian, penambahan zat aditif menggunakan
sorbitol dengan konsentrasi 0,5; 1; 1,5 M dan karakterisasi enzim selulase
sebelum dan setelah penambahan sorbitol. Hasil penelitian menunjukkan bahwa
aktivitas spesifik enzim selulase tanpa sorbitol 35,2038 U/mg, meningkat 10 kali
dibandingkan dengan ekstrak kasar enzim yang mempunyai aktivitas spesifik
3,48078 U/mg. Enzim selulase tanpa sorbitol mempunyai pH optimum 5,5; suhu
optimum 65oC; KM = 18,69 mg/mL substrat; Vmaks = 0,856 ?mol/mL.menit. Uji
stabilitas termal selama 60 menit pada suhu 65oC enzim selulase mempunyai nilai
ki = 0,045 menit-1; waktu paruh (t1/2) = 15,40 menit dan ?Gi = 98,65 kJ/mol.
Enzim setelah penambahan sorbitol mempunyai pH dan suhu optimum yang sama
dengan enzim tanpa sorbitol. Data kinetika enzim setelah penambahan sorbitol 0,5
M diperoleh KM = 17,37 mg/mL, substrat dan Vmaks = 0,596 ?mol/mL.menit, t1/2 =
19,80 menit, ki = 0,035 menit dan ?Gi = 99,32 kJ/mol. Data kinetika enzim
setelah penambahan sorbitol 1 M diperoleh KM = 14,72 mg/mL, substrat dan
Vmaks = 0,342 ?mol/mL.menit, t1/2 = 22,35 menit, ki = 0,031 menit dan ?Gi =
99,65 kJ/mol. Data kinetika enzim setelah penambahan sorbitol 1,5 M diperoleh
KM = 11,33 mg/mL, substrat dan Vmaks = 0,223 ?mol/mL.menit, t1/2 = 27,72 menit,
ki = 0,025 menit dan ?Gi = 100,23 kJ/mol. Berdasarkan penurunan nilai ki,
peningkatan waktu paruh (t1/2) dan nilai ?Gi, diketahui bahwa enzim setelah
ditambahkan senyawa aditif menggunakan sorbitol dapat meningkatkan stabilitas
enzim selulase dari Rhizopus oligosporus.
Kata kunci : Rhizopus oligosporus, selulase, zat aditif, sorbitol



abstract

This research was conducted to determine the stability of the enzyme before and
after the addition of sorbitol compound cellulase enzyme from Rhizopus
oligosporus using the polyol compound sorbitol. Scope of this reseach were
including production, isolation, purification, addition of additives using sorbitol
with a concentration of 0.5; 1.0; 1.5 M and characterization of purified cellulase
enzymes before and after sorbitol addition. The result showed that the purified
enzyme has a specific activity 35,2038 U/mg or increase for 10 times than crude
extract enzyme which has specific activity 3,48078 U/mg respectively. The
purified enzyme have some characteristics are optimum reaction reach at pH 5,5;
temperature 65oC; KM = 18,69 mg/mL; Vmax = 0,856 ?mol/mL.min. A thermal
stability test for 60 min at a temperature of 65oC of cellulase enzyme has ki =
0,043 min-1; t1/2 = 16,11 min and ?Gi = 98,77 kJ/mol. The enzyme after addition
of sorbitol has the same pH and temperature as the purified enzyme. Enzyme
kinetics data after addition of sorbitol 0.5 M were obtained KM = 17,37 mg/mL;
Vmax = 0,596 ?mol/mL.min; ki = 0,039 min-1; t1/2 = 17,76 min and ?Gi = 99,32
kJ.mol-1. Enzyme kinetics data after addition of sorbitol 1 M were obtained KM =
14,72 mg/mL; Vmax = 0,342 ?mol/mL.min; ki = 0,031 min-1; t1/2 = 22,35 min and
?Gi = 99,65 kJ/mol. Enzyme kinetics data after addition of sorbitol 1,5 M were
obtained KM = 11,32 mg/mL; Vmax = 0,223 ?mol/mL.min; ki = 0,029 min-1; t1/2 =
25,66 min and ?Gi = 100,23 kJ/mol. Based on the decrease of ki values, increase
of t1/2 and ?Gi, are known that enzyme after addition sorbitol can increase the
stability of cellulase from Rhizopus oligosporus.
Key words : Rhizopus oligosporus, cellulase, additives, sorbitol
%C UNIVERSITAS LAMPUNG
%D 2017
%I FAKULTAS MATEMATIKA DAN ILMU PENGETAHUAN ALAM
%L eprints28628