?url_ver=Z39.88-2004&rft_val_fmt=info%3Aofi%2Ffmt%3Akev%3Amtx%3Adc&rft.title=AMOBILISASI+ENZIM+SELULASE+DARI+Bacillus+subtilis+ITBCCB148+MENGGUNAKAN+BENTONIT&rft.creator=Fika+Putri+Aulia%2C+1317011027&rft.subject=Q+Science+(General)&rft.description=Selulase+banyak+digunakan+dalam+industri+untuk+mengkonversi+selulosa+menjadi+glukosa.+Agar+dapat+digunakan+dalam+proses+industri%2C+diperlukan+enzim+yang+stabil+pada+pH+dan+suhu+ekstrim.+Penelitian+ini+bertujuan+untuk+meningkatkan+stabilitas+enzim+selulase+dari+isolat+bakteri+Bacillus+subtilis+ITBCCB148+melalui+proses+amobilisasi+enzim+menggunakan+bentonit.+Tahapan+yang+dilakukan+dalam+penelitian+ini+adalah+produksi%2C+isolasi%2C+pemurnian%2C+amobilisasi+dan+karakterisasi+enzim+selulase+hasil+pemurnian+dan+hasil+amobilisasi.+Hasil+penelitian+menunjukkan+aktivitas+spesifik+enzim+hasil+pemurnian+sebesar+14%2C075+U%2Fmg%2C+meningkat+kemurniannya+5%2C7+kali+dibandingkan+ekstrak+kasar+enzim.+Enzim+selulase+hasil+pemurnian+memilik+i+suhu+optimum+50%C2%BAC%2C+sedangkan+enzim+amobil+pada+suhu+60%C2%BAC.+Uji+stabilitas+termal+pada+suhu+60%C2%BAC+selama+100+menit+untuk+enzim+hasil+pemurnian+masih+memiliki+aktivitas+sisa+7%2C330+%25%2C+sedangkan+enzim+amobil+sebesar+14%2C681%25.+Data+kinetika+enzim+hasil+pemurnian+diperoleh+KM+%3D+1%2C278+mg+mL-1+substrat+dan+Vmaks+%3D+1%2C271+%CE%BCmol+mL-1+menit-1%2C+t1%2F2+%3D+31%2C111+menit%2C+ki+%3D+0%2C022+menit-1+dan+%CE%94Gi+%3D100%2C540+KJ+mol-1.+Pada+data+kinetika+enzim+hasil+amobilisasi+diperoleh+KM+%3D+0%2C445+mg+mL-1+substrat+dan+Vmaks+%3D+0%2C422+%CE%BCmol+Ml+menit-1%2C+t1%2F2+%3D+39%2C833+menit%2C+ki+%3D+0%2C017+menit-1+dan+%CE%94Gi+%3D+104%2C424+KJ+mol-1.+Berdasarkan+penurunan+nilai+ki+%2C+peningkatan+waktu+paruh+(t1%2F2)+dan+nilai+%CE%94Gi%2C+diketahui+bahwa+amobilisasi+menggunakan+bentonit+dapat+meningkatkan+stabilitas+enzim+selulase++dari+Bacillus+subtilis+ITBCCB148.%0D%0A%0D%0AKata+kunci+%3A+Selulase+%2C+Bacillus+subtilis+ITBCCB148%2C+Amobilisasi+enzim%2C+Bentonit%0D%0A%0D%0A%0D%0A%0D%0A%0D%0Aabstract%0D%0A%0D%0ACellulase+is+widely+use+in+industry+to+convert+cellulose+into+glucose.+For+a+certain+industrial+processes%2C+the+enzyme+must+be+set+up+at+extreme+level+of+both+pH+and+temperature.+This+research+to+increase+the+stability+of+cellulose+enzyme+from+Bacillus+Subtilis+ITBCCB148+by+the+enzyme+immobilization+process+using+bentonite.+The+steps+in+this+research+are%3A+production%2C+isolation%2C+purification%2C+immobilization+and+characterization+of+purified+and+immobilized+enzymes.+The+research+show+the+specific+activity+of+the+puried+enzyme+of+14%2C075+U%2Fmg%2C+increased+of+5%2C7+times+than+the+crude+extract.+The+purified+cellulase+enzyme+has+an+optimum+temperature+at+500C.+Whereas+the+immobilized+enzyme+at+600C.+The+residual+activity+on+600C+for+100+minutes+for+purified+enzyme+was+7.330%25%2C+while+the+immobilized+enzyme+was+14.681%25.+Kinetic+datas+of+purified+enzyme+results+were+KM+%3D+1.278+mg+mL-1+substrate%2C+Vmax+%3D+1.271+%C2%B5mol+mL-1+minute-1%2C++++++++++++++++++++t1%2F2+%3D+31.111+minutes%2C+Ki+%3D+0.022+min-1+and+%CE%94Gi+%3D+100.540+KJ+mol-1.+The+datas+of+the+immobilized+enzyme+were+KM+%3D+0.445+mg+mL-1+substrate%2C+Vmax+%3D+0.442+%C2%B5mol+mL-1+minute-1%2C+t1%2F2+%3D+39.833+minutes%2C+Ki+%3D+0.017+min-1+and+%CE%94Gi+%3D+104.424+KJ++++++++++mol+-1.+Based+on+the+decrease+of+ki%2C+increase+of+half-time+(t%C2%BD)+and+the+value+of+%CE%94Gi+the+immobilization+by+using+bentonite+can+improve+the+stability+of+cellulose+enzyme+from+Bacillus+Subtilis+ITBCCB148.%0D%0A%0D%0AKey+words+%3A+Cellulase+Enzyme%2C+Bacillus+Subtilis+ITBCCB148%2C+Enzyme+immobilization%2C+Bentonite%0D%0A%0D%0A&rft.publisher=UNIVERSITAS+LAMPUNG&rft.date=2017-11-28&rft.type=Skripsi&rft.type=NonPeerReviewed&rft.format=text&rft.identifier=http%3A%2F%2Fdigilib.unila.ac.id%2F29379%2F1%2FABSTRAK.pdf&rft.format=text&rft.identifier=http%3A%2F%2Fdigilib.unila.ac.id%2F29379%2F19%2FSKRIPSI%2520FULL.pdf&rft.format=text&rft.identifier=http%3A%2F%2Fdigilib.unila.ac.id%2F29379%2F20%2FSKRIPSI%2520TANPA%2520BAB%2520PEMBAHASAN.pdf&rft.identifier=++Fika+Putri+Aulia%2C+1317011027++(2017)+AMOBILISASI+ENZIM+SELULASE+DARI+Bacillus+subtilis+ITBCCB148+MENGGUNAKAN+BENTONIT.++UNIVERSITAS+LAMPUNG%2C+FAKULTAS+MATEMATIKA+DAN+ILMU+PENGETAHUAN+ALAM+.+++++&rft.relation=http%3A%2F%2Fdigilib.unila.ac.id%2F29379%2F