%A 1317011027 Fika Putri Aulia
%T AMOBILISASI ENZIM SELULASE DARI Bacillus subtilis ITBCCB148 MENGGUNAKAN BENTONIT
%X Selulase banyak digunakan dalam industri untuk mengkonversi selulosa menjadi glukosa. Agar dapat digunakan dalam proses industri, diperlukan enzim yang stabil pada pH dan suhu ekstrim. Penelitian ini bertujuan untuk meningkatkan stabilitas enzim selulase dari isolat bakteri Bacillus subtilis ITBCCB148 melalui proses amobilisasi enzim menggunakan bentonit. Tahapan yang dilakukan dalam penelitian ini adalah produksi, isolasi, pemurnian, amobilisasi dan karakterisasi enzim selulase hasil pemurnian dan hasil amobilisasi. Hasil penelitian menunjukkan aktivitas spesifik enzim hasil pemurnian sebesar 14,075 U/mg, meningkat kemurniannya 5,7 kali dibandingkan ekstrak kasar enzim. Enzim selulase hasil pemurnian memilik i suhu optimum 50?C, sedangkan enzim amobil pada suhu 60?C. Uji stabilitas termal pada suhu 60?C selama 100 menit untuk enzim hasil pemurnian masih memiliki aktivitas sisa 7,330 %, sedangkan enzim amobil sebesar 14,681%. Data kinetika enzim hasil pemurnian diperoleh KM = 1,278 mg mL-1 substrat dan Vmaks = 1,271 ?mol mL-1 menit-1, t1/2 = 31,111 menit, ki = 0,022 menit-1 dan ?Gi =100,540 KJ mol-1. Pada data kinetika enzim hasil amobilisasi diperoleh KM = 0,445 mg mL-1 substrat dan Vmaks = 0,422 ?mol Ml menit-1, t1/2 = 39,833 menit, ki = 0,017 menit-1 dan ?Gi = 104,424 KJ mol-1. Berdasarkan penurunan nilai ki , peningkatan waktu paruh (t1/2) dan nilai ?Gi, diketahui bahwa amobilisasi menggunakan bentonit dapat meningkatkan stabilitas enzim selulase  dari Bacillus subtilis ITBCCB148.

Kata kunci : Selulase , Bacillus subtilis ITBCCB148, Amobilisasi enzim, Bentonit




abstract

Cellulase is widely use in industry to convert cellulose into glucose. For a certain industrial processes, the enzyme must be set up at extreme level of both pH and temperature. This research to increase the stability of cellulose enzyme from Bacillus Subtilis ITBCCB148 by the enzyme immobilization process using bentonite. The steps in this research are: production, isolation, purification, immobilization and characterization of purified and immobilized enzymes. The research show the specific activity of the puried enzyme of 14,075 U/mg, increased of 5,7 times than the crude extract. The purified cellulase enzyme has an optimum temperature at 500C. Whereas the immobilized enzyme at 600C. The residual activity on 600C for 100 minutes for purified enzyme was 7.330%, while the immobilized enzyme was 14.681%. Kinetic datas of purified enzyme results were KM = 1.278 mg mL-1 substrate, Vmax = 1.271 ?mol mL-1 minute-1,                    t1/2 = 31.111 minutes, Ki = 0.022 min-1 and ?Gi = 100.540 KJ mol-1. The datas of the immobilized enzyme were KM = 0.445 mg mL-1 substrate, Vmax = 0.442 ?mol mL-1 minute-1, t1/2 = 39.833 minutes, Ki = 0.017 min-1 and ?Gi = 104.424 KJ          mol -1. Based on the decrease of ki, increase of half-time (t?) and the value of ?Gi the immobilization by using bentonite can improve the stability of cellulose enzyme from Bacillus Subtilis ITBCCB148.

Key words : Cellulase Enzyme, Bacillus Subtilis ITBCCB148, Enzyme immobilization, Bentonite


%C FAKULTAS MATEMATIKA DAN ILMU PENGETAHUAN ALAM 
%D 2017
%I UNIVERSITAS LAMPUNG
%L eprints29379