?url_ver=Z39.88-2004&rft_val_fmt=info%3Aofi%2Ffmt%3Akev%3Amtx%3Adc&rft.title=AMOBILISASI+ENZIM+SELULASE+DARI+Aspergillus+niger+L-51%0D%0AMENGGUNAKAN+ZEOLIT&rft.creator=+SINTA+DEWI+O%2C+1317011067&rft.subject=QD+Chemistry&rft.description=proses+sakarifikasi+bahan+berselulosa%2C+deterjen%2C+industri+makanan%2C+dan+pengolahan%0D%0Alimbah+pabrik+kertas.+Penggunaan+enzim+dalam+proses+industri+harus+memenuhi%0D%0Asyarat-syarat+tertentu+yaitu+enzim+harus+stabil+pada+suhu+tinggi+dan+tahan+terhadap%0D%0Akeadaan+pH+ekstrim.+Sedangkan%2C+pada+umumnya+enzim+hanya+mampu+bekerja+pada%0D%0Akondisi+fisiologis+dan+tidak+tahan+terhadap+kondisi+ekstrim.+Penelitian+ini+bertujuan%0D%0Auntuk+memproduksi+dan+mengisolasi+enzim+selulase+dari+jamur+Aspergillus+niger+L-%0D%0A51+yang+kemudian+dimurnikan+dengan+tahap+fraksinasi+dan+dialisis%2C+lalu+ditingkatkan%0D%0Astabilitasnya+melalui+amobilisasi+menggunakan+zeolit.+Aktivitas+spesifik+enzim%0D%0Aselulase+hasil+pemurnian+meningkat+7+kali+lipat+dibandingkan+dengan+ekstrak+kasar%0D%0Aenzim.+Ekstrak+kasar+enzim+memiliki+aktivitas+spesifik+sebesar+5%2C3944+U%2Fmg+dan%0D%0Aenzim+hasil+pemurnian+memiliki+aktivitas+spesifik+sebesar+38%2C0292+U%2Fmg.+Suhu%0D%0Aoptimum+enzim+hasil+pemurnian+pada+suhu+50oC+sedangkan+suhu+optimum+enzim%0D%0Ahasil+amobilisasi+pada+suhu+75oC.+Aktivitas+sisa+yang+dihasilkan+pada+uji+stabilitas%0D%0Atermal+pada+suhu+50oC+selama+100+menit+terhadap+enzim+hasil+pemurnian+adalah%0D%0Asebesar+5%25+sedangkan+terhadap+enzim+hasil+amobilisasi+sebesar+23%25.+Data+kinetika%0D%0Aenzim+hasil+pemurnian+yaitu+Vmaks+%3D+4%2C7400+%CE%BCmol%2FmL%2Fmenit%2C+KM+%3D+1%2C9400+mg%2FmL%2C+ki%0D%0A%3D+0%2C024+menit-1%2C+t1%2F2+%3D+28%2C87+menit-1+dan+%CE%94Gi+%3D+100%2C345+kJ%2Fmol.+Sedangkan+untuk%0D%0Aenzim+hasil+amobilisasi+memiliki+data+kinetika+enzim+yaitu+Vmaks+%3D+1%2C4000%0D%0A%CE%BCmol%2FmL%2Fmenit%2C+KM+%3D+0%2C9900+mg%2FmL%2C+ki+%3D+0%2C013+menit-1%2C+t1%2F2+%3D+53%2C30+menit-1%2C+dan+%CE%94Gi%0D%0A%3D+102%2C175+kJ%2Fmol.+Berdasarkan+hasil+penelitian+ini%2C+disimpulkan+bahwa+enzim+hasil%0D%0Aamobilisasi+menggunakan+zeolit+mengalami+peningkatan+waktu+paruh+sebesar+1%2C84%0D%0Akali+dibandingkan+enzim+hasil+pemurnian.%0D%0AKata+Kunci+%3A+selulase%2C+Aspergillus+niger+L-51%2C+Amobilisasi%2C+zeolit.%0D%0A%0D%0AABSTRACT%0D%0A%0D%0A%0D%0ACellulase+is+a+complex+enzyme+that+cuts+gradually+the+cellulose+chain+into+glucose.+In%0D%0Amany+biotechnology+applications%2C+cellulase+is+used+in+the+process+of+saccharification%0D%0Aof+cellulosic+materials%2C+detergents%2C+food+industry%2C+and+paper+waste+mill+processing.%0D%0AThe+use+of+enzymes+in+industrial+processes+must+satisfy+certain+conditions+i.e%0D%0Aenzymes+must+be+stable+at+high+temperatures+and+resistant+in+extreme+pH+conditions.%0D%0AWhereas%2C+in+general+enzyme+is+only+able+to+work+on+physiological+conditions+and+not%0D%0Aresistant+to+extreme+conditions.+The+purpose+of+this+study+was+to+produce+and+isolate%0D%0Athe+cellulase+enzyme+from+Aspergillus+niger+L-51+fungi+which+is+then+purified+by%0D%0Afractionation+and+dialysis+stage%2C+then+enhanced+its+stability+through+immobilization%0D%0Ausing+zeolite.+The+specific+activity+of+the+purified+cellulase+enzyme+increased+7+times%0D%0Ahiger+than+the+crude+extract+enzyme.+The+crude+extract+enzyme+has+a+specific+activity%0D%0Aof+5.3944+U%2Fmg+and+the+purified+enzyme+has+a+specific+activity+of+38.0292+U%2Fmg.%0D%0AThe+optimum+temperature+of+the+purified+enzyme+was+50oC+while+the+optimum%0D%0Atemperature+of+the+immobilized+enzyme+was+at+75%C2%B0C.+The+residual+activity+resulted+in%0D%0Athermal+stability+test+at+temperature+50oC+for+100+min+against+purification+enzyme%0D%0Awas+5%25+while+the+immobilization+enzyme+of+was+23%25.+The+kinetics+data+of+the%0D%0Apurified+enzyme+were+Vmax+%3D+4.7400+%CE%BCmol%2F+mL%2F+min%2C+KM+%3D+1.9400+mg+%2F+mL%2C+ki+%3D%0D%0A0%2C024+min-1%2C+t1%2F2+%3D+28.87+min-1+and+%CE%94Gi+%3D+100.345+kJ%2Fmol%2C+while+the+immobilization%0D%0Aenzyme+were+Vmax+%3D+1.4000+%CE%BCmol%2FmL%2Fmin%2C+KM+%3D+0.9900+mg%2FmL%2C+ki+%3D+0.013+min-1%2C%0D%0At1%2F2+%3D+53.30+min-1%2C+and+%CE%94Gi+%3D+102.175+kJ%2Fmol.+Based+on+the+results+of+this+study%2C+it%0D%0Awas+concluded+that+the+amobilization+enzyme+of+using+zeolite+had+an+increase+in+halflife%0D%0Aof+1.84+times+compared+to+the+purification+enzyme.%0D%0AKey+Word+%3A+Cellulase%2C+Aspergillus+niger+L-51%2C+Immobilization%2C+zeolite.&rft.publisher=FAKULTAS+MATEMATIKA+DAN+ILMU+PENGETAHUAN+ALAM+&rft.date=2017-12-27&rft.type=Skripsi&rft.type=NonPeerReviewed&rft.format=text&rft.identifier=http%3A%2F%2Fdigilib.unila.ac.id%2F29860%2F1%2FABSTRAK.pdf&rft.format=text&rft.identifier=http%3A%2F%2Fdigilib.unila.ac.id%2F29860%2F2%2FSKRIPSI%2520FULL.pdf&rft.format=text&rft.identifier=http%3A%2F%2Fdigilib.unila.ac.id%2F29860%2F3%2FSKRIPSI%2520TANPA%2520BAB%2520PEMBAHASAN.pdf&rft.identifier=+++SINTA+DEWI+O%2C+1317011067++(2017)+AMOBILISASI+ENZIM+SELULASE+DARI+Aspergillus+niger+L-51+MENGGUNAKAN+ZEOLIT.++FAKULTAS+MATEMATIKA+DAN+ILMU+PENGETAHUAN+ALAM+%2C+UNIVERSITAS+LAMPUNG.+++++&rft.relation=http%3A%2F%2Fdigilib.unila.ac.id%2F29860%2F