TY  - GEN
CY  - UNIVERSITAS LAMPUNG
ID  - eprints29860
UR  - http://digilib.unila.ac.id/29860/
A1  -  SINTA DEWI O, 1317011067
Y1  - 2017/12/27/
N2  - proses sakarifikasi bahan berselulosa, deterjen, industri makanan, dan pengolahan
limbah pabrik kertas. Penggunaan enzim dalam proses industri harus memenuhi
syarat-syarat tertentu yaitu enzim harus stabil pada suhu tinggi dan tahan terhadap
keadaan pH ekstrim. Sedangkan, pada umumnya enzim hanya mampu bekerja pada
kondisi fisiologis dan tidak tahan terhadap kondisi ekstrim. Penelitian ini bertujuan
untuk memproduksi dan mengisolasi enzim selulase dari jamur Aspergillus niger L-
51 yang kemudian dimurnikan dengan tahap fraksinasi dan dialisis, lalu ditingkatkan
stabilitasnya melalui amobilisasi menggunakan zeolit. Aktivitas spesifik enzim
selulase hasil pemurnian meningkat 7 kali lipat dibandingkan dengan ekstrak kasar
enzim. Ekstrak kasar enzim memiliki aktivitas spesifik sebesar 5,3944 U/mg dan
enzim hasil pemurnian memiliki aktivitas spesifik sebesar 38,0292 U/mg. Suhu
optimum enzim hasil pemurnian pada suhu 50oC sedangkan suhu optimum enzim
hasil amobilisasi pada suhu 75oC. Aktivitas sisa yang dihasilkan pada uji stabilitas
termal pada suhu 50oC selama 100 menit terhadap enzim hasil pemurnian adalah
sebesar 5% sedangkan terhadap enzim hasil amobilisasi sebesar 23%. Data kinetika
enzim hasil pemurnian yaitu Vmaks = 4,7400 ?mol/mL/menit, KM = 1,9400 mg/mL, ki
= 0,024 menit-1, t1/2 = 28,87 menit-1 dan ?Gi = 100,345 kJ/mol. Sedangkan untuk
enzim hasil amobilisasi memiliki data kinetika enzim yaitu Vmaks = 1,4000
?mol/mL/menit, KM = 0,9900 mg/mL, ki = 0,013 menit-1, t1/2 = 53,30 menit-1, dan ?Gi
= 102,175 kJ/mol. Berdasarkan hasil penelitian ini, disimpulkan bahwa enzim hasil
amobilisasi menggunakan zeolit mengalami peningkatan waktu paruh sebesar 1,84
kali dibandingkan enzim hasil pemurnian.
Kata Kunci : selulase, Aspergillus niger L-51, Amobilisasi, zeolit.

ABSTRACT


Cellulase is a complex enzyme that cuts gradually the cellulose chain into glucose. In
many biotechnology applications, cellulase is used in the process of saccharification
of cellulosic materials, detergents, food industry, and paper waste mill processing.
The use of enzymes in industrial processes must satisfy certain conditions i.e
enzymes must be stable at high temperatures and resistant in extreme pH conditions.
Whereas, in general enzyme is only able to work on physiological conditions and not
resistant to extreme conditions. The purpose of this study was to produce and isolate
the cellulase enzyme from Aspergillus niger L-51 fungi which is then purified by
fractionation and dialysis stage, then enhanced its stability through immobilization
using zeolite. The specific activity of the purified cellulase enzyme increased 7 times
higer than the crude extract enzyme. The crude extract enzyme has a specific activity
of 5.3944 U/mg and the purified enzyme has a specific activity of 38.0292 U/mg.
The optimum temperature of the purified enzyme was 50oC while the optimum
temperature of the immobilized enzyme was at 75°C. The residual activity resulted in
thermal stability test at temperature 50oC for 100 min against purification enzyme
was 5% while the immobilization enzyme of was 23%. The kinetics data of the
purified enzyme were Vmax = 4.7400 ?mol/ mL/ min, KM = 1.9400 mg / mL, ki =
0,024 min-1, t1/2 = 28.87 min-1 and ?Gi = 100.345 kJ/mol, while the immobilization
enzyme were Vmax = 1.4000 ?mol/mL/min, KM = 0.9900 mg/mL, ki = 0.013 min-1,
t1/2 = 53.30 min-1, and ?Gi = 102.175 kJ/mol. Based on the results of this study, it
was concluded that the amobilization enzyme of using zeolite had an increase in halflife
of 1.84 times compared to the purification enzyme.
Key Word : Cellulase, Aspergillus niger L-51, Immobilization, zeolite.
PB  - FAKULTAS MATEMATIKA DAN ILMU PENGETAHUAN ALAM 
TI  - AMOBILISASI ENZIM SELULASE DARI Aspergillus niger L-51
MENGGUNAKAN ZEOLIT
AV  - restricted
ER  -