?url_ver=Z39.88-2004&rft_val_fmt=info%3Aofi%2Ffmt%3Akev%3Amtx%3Adc&rft.title=STUDY+THE+EFFECT+OF+ADDITION+SORBITOL+TOWARD+STABILITY%0D%0AOF+CELLULASE+ENZYME+FROM+Rhizopus+oryzae&rft.creator=DIANI+ISKA+MIRANTI%2C+1217011015&rft.subject=QD+Chemistry&rft.description=This+research+aims+to+study+the+effect+of+addition+sorbitol+toward+stability+cellulose%0D%0Aenzyme+from+Rhizopus+oryzae.+To+approach+this+aims%2C+the+enzyme+has+been+done%0D%0Ato+produce%2C+to+isolate+and+to+purify.+The+purification+of+cellulose+enzyme+was%0D%0Adone+by+fractination+using+the+ammonium+sulfat+and+dialysis.+The+purified+enzyme%0D%0Awas+mixed+by+sorbitol.+Determination+of+the+cellulose+enzyme+activity+performed%0D%0Aby+the+method+of+Mandels%2C+while+the+measurement+of+protein+concentration%0D%0Aperformed+by+the+method+of+Lowry.%0D%0AThe+results++showed++that++the++purified+enzyme++has++specific++activity+9.18+U%2Fmg%2C%0D%0Aincreased+4.03++times++than+the+native+enzyme.+The+test+of+thermal+stability+of+the%0D%0Aenzyme+at+temperature+50%C2%B0C+for+60+minutes+has+k%0D%0Ai%0D%0A%3D+0.02+min%0D%0A-1%0D%0A%3B+t%0D%0A%3D+34.65+min%0D%0Aand+%CE%94G%0D%0Ai%0D%0A%3D+99.7+kJ+mol%0D%0A-1%0D%0A.+The+test+of+thermal+stability+of+the+enzyme+mixed+by%0D%0Asorbitol+0.5%3B+1.0%3B+and+1.5+M+at+temperature+50%C2%B0C+for+60+minutes+each+has+t%0D%0A%3D+49.5%0D%0Amin%2C+k%0D%0Ai%0D%0A%3D+0.014+min%0D%0A-1%0D%0Aand+%CE%94G%0D%0Ai%0D%0A%3D+100.+kJ+mol%0D%0A%3B%0D%0At%0D%0A1%2F2%0D%0A%3D+24.75+min%2C+k%0D%0Ai%0D%0A%3D+0.028+min%0D%0A-1%0D%0Aand+%CE%94G%0D%0Ai%0D%0A-1%0D%0A%3D+101.8+kJ+mol%0D%0A-1%0D%0A%3B+t%0D%0A%3D+22.35+min%2C%0D%0Aki+%3D+0.031+min%0D%0A-1%0D%0Aand+%CE%94Gi+%3D+100.8+kJ+mol%0D%0AThe+results+showed+that+the+purified+enzyme+has+K%0D%0A-1%0D%0A.%0D%0Am%0D%0Aand+V%0D%0Avalues%0D%0A74.02+mg%2FmL+substrate+and+6.57%0D%0A1%2F2%0D%0Amax%0D%0A1%2F2%0D%0A%CE%BCmol%2FmL.min%0D%0A%2C+and+the+enzyme+mixed+by+sorbitol%0D%0A0.5%3B+1.0%3B+and+1.5+M+each+has%0D%0A24.89+mg%2FmL+substrate+and+0.92+%CE%BCmol%2FmL.min%3B%0D%0A18.39+mg%2FmL+substrate+and+0.82+%CE%BCmol%2FmL.min%3B+14.83+mg%2FmL+substrate+and+0.42%0D%0A%CE%BCmol%2FmL.min.%0D%0AAlthough+the+optimum+of+pH+and+temperature+of+the+mixed+enzyme+by+sorbitol+did%0D%0Anot+changed%2C+at+pH+7+and+at+temperature+60%0D%0Ao%0D%0AC+but+the+thermal+stability+of%0D%0Amodified+enzyme+was+increased+and+showed+by+the+decrease+in+the+value+of+k%0D%0Ai%0D%0Ain+accordance+with+the+increase+half+life+and+%CE%94G%0D%0AKey+word%3A+Cellulase+enzyme%2C+sorbitol%2C+Rhizopus+oryzae%0D%0Ai%0D%0A.%0D%0A1%2F2%0D%0A%0D%0A%0D%0AABSTRAK%0D%0A%0D%0A%0D%0APenelitian+ini+bertujuan+untuk+mempelajari+pengaruh+penambahan+sorbitol+terhadap%0D%0Astabilitas+enzim+selulase+dari+Rhizopus+oryzae.+Untuk+mencapai+tujuan+tersebut%0D%0Adilakukan+produksi%2C+isolasi%2C+dan+pemurnian+enzim.+Pemurnian+enzim+selulase+dilakukan%0D%0Adengan+menggunakan+fraksinasi+dengan+ammonium+sulfat+dan+dialisis.+Enzim+hasil%0D%0Apemurnian+kemudian+ditambahkan+sorbitol+dengan+konsentrasi+0%2C5+M%3B+1%2C0+M%3B+dan+1%2C5+M.%0D%0APenentuan+aktivitas+enzim+selulase+dilakukan+dengan+metode+Mandels+sedangkan%0D%0Apengukuran+kadar+protein+dilakukan+dengan+metode+Lowry.%0D%0AHasil+penelitian+menunjukkan+bahwa+enzim+hasil+pemurnian+memiliki+aktivitas+spesifik%0D%0Asebesar+9%2C18+U%2Fmg%2C+meningkat+4%2C03+kali+dibandingkan+ekstrak+kasar+enzim.+Uji+stabilitas%0D%0Atermal+enzim+hasil+pemurnian+pada+suhu+50%0D%0A%3Bt%0D%0A1%2F2%0D%0A%3D+34%2C65+menit%3B+dan+%E2%88%86G%0D%0Ai%0D%0Ao%0D%0AC+selama+60+menit+memiliki+k%0D%0A%3D+99%2C7+kJmol%0D%0A-1%0D%0A.+Uji+stabilitas+termal+enzim+setelah%0D%0Apenambahan+sorbitol+0%2C5%3B+1%2C0%3B+dan+1%2C5+M+pada+suhu+50%0D%0Ao%0D%0AC+selama+60+menit+memiliki%0D%0Aberturut-turut+t%0D%0A1%2F2%0D%0A%3D+49%2C5+menit%2C+k%0D%0Ai%0D%0A%3D+0%2C014+menit%0D%0A-1%0D%0Adan%0D%0A%E2%88%86G%0D%0A%3D+24%2C75%0D%0Amenit%2C+k%0D%0Adan%0D%0A%E2%88%86G%0D%0Ai%0D%0Ai%0D%0A%3D+0%2C028+menit%0D%0A%3D+100%2C8+kJmol%0D%0A-1%0D%0A-1%0D%0Adan%0D%0A%E2%88%86%0D%0AG%0D%0Ai%0D%0A%3D+101%2C8+kJmol%0D%0A-1%0D%0A%3B%0D%0At%0D%0A1%2F2%0D%0Ai%0D%0A%3D+100+kJmol%0D%0A%3D+22%2C35+menit%2C+k%0D%0A.%0D%0AEnzim+hasil+pemurnian+memiliki+nilai+Km+dan+Vmaks+berturut-turut+sebesar+74%2C02%0D%0Amg%2FmL+substrat+dan+6%2C57+%CE%BCmol%2FmL.menit%2C+sedangkan+enzim+setelah+peambahan+sorbitol%0D%0A0%2C5%3B+1%2C0%3B+dan+1%2C5+M%0D%0Amemiliki+nilai+Km+dan+Vmaks+berturut-turut+sebesar+24%2C89+mg%2FmL%0D%0Asubstrat+dan+0%2C92+%CE%BCmol%2FmL.menit%3B+18%2C39+mg%2FmL+substrat+dan+0%2C82+%CE%BCmol%2FmL.menit%3B%0D%0A14%2C83+mg%2FmL+substrat+dan+0%2C42+%CE%BCmol%2FmL.menit.%0D%0AWalaupun+suhu+optimum+dan+pH+optimum+enzim+setelah+penambahan+sorbitol+tidak%0D%0Amengalami+perubahan%2C+yakni+tetap+pada+pH+7+dan+suhu+60%0D%0Ao%0D%0AC+tetapi+terjadi+peningkatan%0D%0Astabilitas+termal+karena+adanya+penurunan+nilai+k%0D%0A%E2%88%86G%0D%0Ai%0D%0A.%0D%0AKata+Kunci%3A+Enzim+selulase%2C+sorbitol%2C+Rhizopus+oryzae.%0D%0Ai%0D%0Ai%0D%0A%3D+0%2C02+menit%0D%0A-1%0D%0A%3B+t%0D%0A1%2F2%0D%0A%3D+0%2C031+menit%0D%0Ai%0D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