?url_ver=Z39.88-2004&rft_val_fmt=info%3Aofi%2Ffmt%3Akev%3Amtx%3Adc&rft.title=PENETAPAN+WAKTU+INKUBASI+OPTIMUM+DEGRADASI+KITIN+OLEH+KITINASE+DARI+Actinomycetes+ANL-4+SECARA+SPEKTROFOTOMETRI+UV-Vis&rft.creator=Utami+Putri+Heriyani%2C+1017011064&rft.subject=+&rft.description=Chitin+is+an+insoluble+polymer+which+composed+by+%CE%B2-1%2C4-N-asetil-D-glucosamine+(GlcNAc)+residues.+Chitin+can+be+isolated+from+shirmp+shells+through+two+processes%2C+namely+deproteinization+and+demineralization.+Furthermore%2C+chitin+can+be+hidrolyzed+as+its+monomers+and+oligomers+by+chitinase+enzyme+from+Actinomycetes+ANL-4.+The+aim+of+this+research+is+to+determine+incubation+time+of+chitin+degradation+by+enzyme+chitinases+based+on+the+amount+of+glucosamine+which+is+obtained+every+5+days.+Chitin+degradation+processes+used+a+batch+fermentation+during+45+days.+Glucosamine+from+fermentation+process+is+derivatived+using+phenyl+isothiocyanate+(PITC)+to+be+phenyl+thiourea+(PTH).+PTH+absorbance+is+measured+by+spectrofotometry+UV-Visible+at+%CE%BB+maximum+273+nm.+The+results+are+plotted+to+the+linear+regression+equation+y+%3D+0%2C0697x+%E2%80%93+0%2C0007.+The+highest+glucosamine+yields+is+obtained+at+15+days+incubation+time+with+yield+percents+71%2C70+%25+and+glucosamine+content+in+the+yield+was+95%2C50+%25.+The+purity+of+glucosamine+is+analyzed+using+HPLC-ELSD.+The+HPLC-ELSD+glucosamine+cromatogram+from+15+days+incubation+time+shown+that+there+are+two+peaks+at+2-3+minutes+retention+time%2C+which+is+one+dominant+peak+with+high+intensity.+So+it+can+be+conclude+that+15+days+incubation+is+the+optimum+incubation+time+for+chitin+degraration+by+Actinomycetes+ANL-4.%0D%0A%0D%0AKitin+merupakan+suatu+polimer+tak+larut+yang+tersusun+dari+residu+%CE%B2-1%2C4-N-asetil-D-glukosamin+(GlcNAc).+Kitin+dapat+diisolasi+dari+kulit+udang+melalui+dua+tahapan+proses%2C+yaitu+deproteinasi+dan+demineralisasi.+Selanjutnya+kitin+hasil+isolasi+dapat+didegradasi+menjadi+monomer-monomer+dan+oligomernya+oleh+enzim+kitinase+dari+Actinomycetes+ANL-4.+Tujuan+dari+penelitian+ini+adalah+untuk+menentukan+waktu+inkubasi+optimum+degradasi+kitin+oleh+enzim+kitinase+dari+Actinomycetes+ANL-4+berdasarkan+jumlah+glukosamin+yang+diperoleh+setiap+5+hari+inkubasi.+Proses+degradasi+kitin+menggunakan+suatu+tahap+fermentasi+cair+sistem+tertutup+yang+berlangsung+selama+45+hari.+Glukosamin+dalam+rendemen+hasil+fermentasi+diderivatisasi+menggunakan+senyawa+fenil+isotiosianat+menjadi+fenil+tiourea+(PTH).+Absorbansi+PTH+diukur+menggunakan+spektrofotometri+UV-Vis+pada+%CE%BB+maks+273+nm.+Hasil+pengukuran+diplotkan+ke+dalam+persamaan+regresi+linear+y+%3D+0%2C0697x+%E2%80%93+0%2C0007.+Bobot+rendemen+glukosamin+tertinggi+diperoleh+pada+hari+ke+15+inkubasi+dengan+persentase+rendemen+71%2C70%25+dengan+kadar+glukosamin+didalam+rendemennya+adalah+95%2C50%25.+Glukosamin+hasil+fermentasi+dianalisis+kemurniannya+menggunakan+HPLC-ELSD.+Kromatogram+HPLC-ELSD+glukosamin+hasil+fermentasi+hari+ke+15+inkubasi+menunjukkan+adanya+dua+puncak+pada+waktu+retensi+2-3+menit%2C+dimana+terdapat+satu+puncak+glukosamin+yang+dominan+dengan+yang+intensitas+tinggi.+Jadi+dapat+disimpulkan+bahwa+hari+ke+15+merupakan+waktu+inkubasi+optimum+degradasi+kitin+oleh+Actinomycetes+ANL-4.&rft.publisher=FAKULTAS+MATEMATIKA+DAN+ILMU+PENGETAHUAN+ALAM&rft.date=2014-08-27&rft.type=Skripsi&rft.type=NonPeerReviewed&rft.format=text&rft.identifier=http%3A%2F%2Fdigilib.unila.ac.id%2F3190%2F1%2FABSTRACT.pdf&rft.format=text&rft.identifier=http%3A%2F%2Fdigilib.unila.ac.id%2F3190%2F2%2FABSTRAK.pdf&rft.format=text&rft.identifier=http%3A%2F%2Fdigilib.unila.ac.id%2F3190%2F3%2FCOVER%2520DALAM.pdf&rft.format=text&rft.identifier=http%3A%2F%2Fdigilib.unila.ac.id%2F3190%2F4%2FCOVER%2520LUAR.pdf&rft.format=text&rft.identifier=http%3A%2F%2Fdigilib.unila.ac.id%2F3190%2F5%2FDAFTAR%2520ISI.pdf&rft.format=text&rft.identifier=http%3A%2F%2Fdigilib.unila.ac.id%2F3190%2F6%2FDAFTAR%2520TABEL.pdf&rft.format=text&rft.identifier=http%3A%2F%2Fdigilib.unila.ac.id%2F3190%2F7%2FDAFTAR%2520GAMBAR.pdf&rft.format=text&rft.identifier=http%3A%2F%2Fdigilib.unila.ac.id%2F3190%2F8%2FHALAMAN%2520PERSETUJUAN.pdf&rft.format=text&rft.identifier=http%3A%2F%2Fdigilib.unila.ac.id%2F3190%2F9%2FHALAMAN%2520PENGESAHAN.pdf&rft.format=text&rft.identifier=http%3A%2F%2Fdigilib.unila.ac.id%2F3190%2F10%2FMOTO.pdf&rft.format=text&rft.identifier=http%3A%2F%2Fdigilib.unila.ac.id%2F3190%2F11%2FPERSEMBAHAN.pdf&rft.format=text&rft.identifier=http%3A%2F%2Fdigilib.unila.ac.id%2F3190%2F12%2FRIWAYAT%2520HIDUP.pdf&rft.format=text&rft.identifier=http%3A%2F%2Fdigilib.unila.ac.id%2F3190%2F13%2FSANWACANA.pdf&rft.format=text&rft.identifier=http%3A%2F%2Fdigilib.unila.ac.id%2F3190%2F14%2FBAB%2520I.pdf&rft.format=text&rft.identifier=http%3A%2F%2Fdigilib.unila.ac.id%2F3190%2F15%2FBAB%2520II.pdf&rft.format=text&rft.identifier=http%3A%2F%2Fdigilib.unila.ac.id%2F3190%2F16%2FBAB%2520III.pdf&rft.format=text&rft.identifier=http%3A%2F%2Fdigilib.unila.ac.id%2F3190%2F17%2FBAB%2520IV.pdf&rft.format=text&rft.identifier=http%3A%2F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