?url_ver=Z39.88-2004&rft_val_fmt=info%3Aofi%2Ffmt%3Akev%3Amtx%3Adc&rft.title=ISOLASI+DAN+KARAKTERISASI+ENZIM+PROTEASE+DAN+UJI+KEMAMPUAN+ANTIBIOTIK+MIKROBA+DARI+TAUCO&rft.creator=AYU+IMANI%2C+1217011010&rft.subject=Q+Science+(General)&rft.description=Tauco+merupakan+salah+satu+pangan+fermentasi+yang+mempunyai+bakteri+penghasil+protease+yang+berpotensi+untuk+dieksplor.+Penelitian+ini+bertujuan+utuk+mengetahui+isolat+bakteri+proteolitik+yang+menghasilkan+enzim+protease+tertinggi.+Berdasarkan+hasil+isolasi+dengan+metode+spread+plate+diperoleh+15+isolat+kandidat.+Setelah+melalui+tahap+skrining+dengan+metode+streak+plate+berdasarkan+indeks+proteolitik+dan+pengukuran+aktivitas+unit+enzim+dengan+metode+Kunitz+terpilih+tiga+isolat+yaitu+isolat+A.4.6%2C+A.5.2+dan+A.5.8.+Tiga+isolat+terpilih+menunjukan+aktivitas+enzim+yang+tinggi+dan+aktivitas+tertinggi+pada+isolat+A.5.2+yaitu+15336+U%2FmL+dan+14432+U%2Fmg+untuk+aktivitas+unit+dan+aktivitas+spesifiknya+berturut-turut.+Tiga+isolat+terpilih+juga+menunjukkan+aktivitas+antibiotik+dengan+zona+hambat+tertinggi+pada+isolat+A.5.2+yaitu+sebesar+5%2C6+mm+diameter.+Tiga+isolat+terpilih+bersifat+motil+dan+berjenis+gram+negatif.%0D%0AKata+kunci%3A+Bakteri+Proteolitik%2C+Enzim+Protease%2C+Fermentasi+Tauco%2C+Degradasi+protein.%0D%0A%0D%0AISOLATION+AND+CHARACTERIZATION+OF+PROTEASE+ENZYM+AND+ANTIBIOTIC+ACTIVITIES+MICROORGANISM+FROM+TAUCO%0D%0A%0D%0A%0D%0ATauco+is+one+of+fermented+foods+that+has+protease+producing+bactery+potentially+to+be+explored.+This+study+was+aimed+to+select+isolates+of+proteolytic+bacteria+that+produce+high+protease+enzymes.+Based+on+isolation+results+with+spread+plate+method+15+candidates+were+obtained.+After+screening+stage+using+streak+plate+method%2C+based+on+the+proteolytic+index+and+its+unit+activity+of+the+enzyme+that+measured+by+Kunitz+method%2C+three+isolates+were+selected.+The+isolates+were+A.4.6%2C+A.5.2+and+A.5.8.+The+isolates+showed+the+high+enzyme+activity+and+the+highest+activity+was+achieved+by+isolate+A.5.2+that+were+15336+U%2FmL+and+14432+U%2Fmg+for+it%E2%80%99s+unit+and+specific+activity%2C+respectively.+The+three+selected+isolates+also+showed+antibiotic+activity+with+the+highest+inhibiting+zone+for+isolate+A.5.2+of+5.6+mm+in+diameter.+Three+selected+isolates+were+motile+and+gram-negative.%0D%0AKeywords%3A+Proteolytic+Bacteria%2C+Protease+Enzyme%2C+Tauco+Fermentation%2C+Degradation+of+Protease.&rft.publisher=FAKULTAS+MATEMATIKA+DAN+ILMU+PENGETAHUAN+ALAM&rft.date=2018-06-07&rft.type=Skripsi&rft.type=NonPeerReviewed&rft.format=text&rft.identifier=http%3A%2F%2Fdigilib.unila.ac.id%2F32825%2F1%2FABSTRAK.pdf&rft.format=text&rft.identifier=http%3A%2F%2Fdigilib.unila.ac.id%2F32825%2F19%2FSKRIPSI%2520FULL.pdf&rft.format=text&rft.identifier=http%3A%2F%2Fdigilib.unila.ac.id%2F32825%2F20%2FSKRIPSI%2520TANPA%2520BAB%2520PEMBAHASAN.pdf&rft.identifier=++AYU+IMANI%2C+1217011010++(2018)+ISOLASI+DAN+KARAKTERISASI+ENZIM+PROTEASE+DAN+UJI+KEMAMPUAN+ANTIBIOTIK+MIKROBA+DARI+TAUCO.++FAKULTAS+MATEMATIKA+DAN+ILMU+PENGETAHUAN+ALAM%2C+UNIVERSITAS+LAMPUNG.+++++&rft.relation=http%3A%2F%2Fdigilib.unila.ac.id%2F32825%2F