?url_ver=Z39.88-2004&rft_val_fmt=info%3Aofi%2Ffmt%3Akev%3Amtx%3Adc&rft.title=KONVERSI+ENZIMATIS+PATI+SAGU+MENJADI+GLUKOSA%0D%0AMENGGUNAKAN+ENZIM+%EF%81%A1-AMILASE+DARI+Bacillus+Subtilis%0D%0AITBCCB148+YANG+DIAMOBILISASI+DENGAN+KITOSAN+UNTUK%0D%0APRODUKSI+BIOETANOL&rft.creator=NI+PUTU+RAHMA+AGUSTINA%2C++1417011080&rft.subject=Q+Science+(General)&rft.subject=QD+Chemistry&rft.description=Kebutuhan+bahan+bakar+saat+ini+semakin+meningkat+sementara+ketersediaan%0D%0Abahan+bakar+fosil+terus+berkurang.+Oleh+karena+itu%2C+diperlukan+sumber+energi%0D%0Aalternatif+untuk+mengatasi+masalah+tersebut.+Bioetanol+merupakan+salah+satu%0D%0Asumber+energi+alternatif+yang+dapat+diperbaharui+dan+berpeluang+untuk%0D%0Amenggantikan+sumber+energi+yang+tidak+dapat+diperbaharui.+Penelitian+ini%0D%0Abertujuan+untuk+memanfaatkan+pati+sagu+sebagai+bahan+baku+untuk+memproduksi%0D%0Abioetanol.+Pati+sagu+dikonversi+menggunakan+enzim+%CE%B1-amilase+dari+Bacillus%0D%0Asubtilis+ITBCCB148+yang+diamobilisasi+dengan+matriks+kitosan+melalui+metode%0D%0Aikatan+silang+menjadi+glukosa.+Tahap+penelitian+ini+meliputi+proses+isolasi+enzim%2C%0D%0Apemurnian+enzim%2C+amobilisasi+enzim%2C+karakterisasi+enzim+hasil+pemurnian+dan%0D%0Aamobilisasi+serta+konversi+enzimatis+pati+sagu+dan+fermentasi+bioetanol+dengan%0D%0Abantuan+Saccharomyces+cerevisiae.%0D%0AHasil+penelitian+menunjukkan+bahwa+aktivitas+spesifik+enzim+%CE%B1-amilase+hasil%0D%0Apemurnian+hingga+tahap+dialisis+adalah+4.576%2C43+Umg-1+dan+kemurniannya%0D%0Ameningkat+9+kali+dibandingkan+dengan+ekstrak+kasar+enzimnya.+Enzim+%CE%B1-amilase%0D%0Ahasil+pemurnian+memiliki+suhu+optimum+65oC%2C+KM+%3D+2%2C037+mgmL-1+substrat%2C+dan%0D%0AVmaks+%3D+28%2C571%CE%BCmolmL-1+menit-1.+Enzim+%CE%B1-amilase+hasil+amobilisasi+memiliki%0D%0Asuhu+optimum75oC%2C+KM+%3D+2%2C046+mgmL-1+substrat%2C+dan+Vmaks%3D+27%2C027+%CE%BCmolmL-1%0D%0Amenit-1.+Aktivitas+sisa+enzim+hasil+pemurnian+dan+hasil+amobilisasi+dalam+uji%0D%0Astabilitas+termal+pada+suhu+60oC+selama+80+menit+berturut-turut+sebesar+21%25+dan%0D%0A81%25.+Enzim+amobil+dapat+digunakan+berulang+hingga+5+kali.+Data+kinetika+enzim%0D%0Ahasil+pemurnian+diperoleh+waktu+paruh+(t1%2F2)%3D+32%2C38+menit%2C+ki%3D+0%2C0214+menit-1%2C+dan%0D%0A%CE%94Gi%3D+177%2C7+kJmol-1.+Data+kinetika+enzim+hasil+amobilisasi+diperoleh+t%C2%BD+%3D+256%2C67%0D%0Amenit%2C+ki+%3D+0%2C0027+menit-1%2C+dan+%CE%94Gi+yaitu+214%2C8+kJ+mol-1.+Berdasarkan+penurunan%0D%0Anilai+ki+%2C+peningkatan+nilai+%CE%94Gi+dan+t1%2F2%2C+diketahui+bahwa+amobilisasi+menggunakan%0D%0Akitosan+dapat+meningkatkan+stabilitas+enzim+%CE%B1-amilase+dari+B.+subtilis%0D%0AITBCCB148.+Selanjutnya%2C+hasil+fermentasi+S.+cerevisiae+pada+pati+sagu+yang%0D%0Adikonversi+dengan+enzim+%CE%B1-amilase+didapatkan+kadar+etanol+sebesar+0%2C26%25+(w%2Fv).%0D%0AKata+kunci+%3A+%EF%81%A1-amilase%2C+amobilisasi%2C+B.+subtilis+ITBCCB148%2C+kitosan%2C%0D%0AS.+cerevisiae%0D%0A%0D%0Aabstract%0D%0A%0D%0ACurrent+fossil+fuel+demand+are+increasing+recently+however+the+supply+of+that%0D%0Afuels+became+decreasing.+An+alternative+energy+sources+are+intended+to+address%0D%0Aconcerns+about+fossil+fuels.+Bioethanol+is+one+of+the+alternative+energy+sources%0D%0Athat+is+renewable+and+has+a+chance+to+subtitute+un-renewable+energy+sources+such%0D%0Aas+fossil+fuels.+The+aims+of+this+study+were+to+utilization+of+sago+starch+as+raw%0D%0Amaterial+for+bioethanol+production.+Sago+converted+using+%CE%B1-amylase+from%0D%0ABacillus+subtilis+ITBCCB148+that+was+immobilized+through+crosslinking+method%0D%0Aon+chitosan+matrix+into+glucose.+The+sequential+processes+applied+as+following%3A%0D%0Aenzyme+isolation%2C+enzyme+purification%2C+enzyme+immobilization%2C+characterization%0D%0Aof+the+purified+and+immobilized+enzyme%2C+saccharification+of+sago+starch+and%0D%0Abioethanol+fermentation+using+Saccharomyces+cerevisiae.%0D%0AThe+results+showed+that+the+specific+activity+of+purified+%CE%B1-amylase+enzyme%0D%0Aafter+the+dialysis+stage+was+4576.43+Umg-1+and+its+purity+9+times+higher+than+the%0D%0Acrude+extract+enzyme.+The+purified+%CE%B1-amylase+enzyme+has+an+optimum%0D%0Atemperature+of+65oC%2C+KM+%3D+2.037+mgmL-1+substrate%2C+dan+Vmaks+%3D+28.571%0D%0A%CE%BCmolmL-1+min-1.+The+immobilized+%CE%B1-amylase+enzyme+has+an+optimum%0D%0Atemperature+of+75oC%2C+KM+%3D+2.046+mgmL-1+substrate%2C+dan+Vmaks%3D+27.027+%CE%BCmolmL-1%0D%0Amin-1.+The+residual+activity+of+the+purified+enzyme+and+immobilized+enzyme+in+the%0D%0Astudy+of+thermal+stability+on+60oC+for+80+minutes+were+21%25+dan+81%25+respectively.%0D%0AThe+immobilized+enzyme+can+be+used+repeadly+up+to+5+times.+The+kinetic+studies%0D%0Aof+the+purified+enzyme+were+obtained+half-life+(t1%2F2+)+was+32.38+minutes%2C+ki+was%0D%0A0.0214+min-1%2C+and+%CE%94Gi+was+177.7+kJ+mol-1.+The+investigation+of+the+kinetic+studies%0D%0Aof+immobilized+enzyme+showed+that+t%C2%BD+was+256.67+minutes%2C+ki+was+0.0027+min-1%2C%0D%0Aand+%CE%94Giwas+214.8+kJmol-1.+Based+on+the+decreasing+of+ki+value%2C+increasing+of+%CE%94Gi%0D%0Aand+t1%2F2%2C+the+immobilization+by+chitosan+in+this+study+improved+the+stability+of+%CE%B1-%0D%0Aamylase+from+B.+subtilis+ITBCCB148.+Futhermore%2C+the+S.+cerevisiae+fermentation%0D%0Aon+sago+starch+converted+by+the+immobilized+%CE%B1-amylase+enzyme+obtained+ethanol%0D%0Aapproximately+0%2C26%25+(w%2Fv).%0D%0AKeywords+%3A+%CE%B1-amylase%2C+immobilization%2C+B.+subtilis+ITBCCB148%2C+chitosan%2C%0D%0AS.+cerevisiae&rft.publisher=FAKULTAS+MATEMATIKA+DAN+ILMU+PENGETAHUAN+ALAM+&rft.date=2018-08-02&rft.type=Skripsi&rft.type=NonPeerReviewed&rft.format=text&rft.identifier=http%3A%2F%2Fdigilib.unila.ac.id%2F33171%2F1%2FABSTRAK.pdf&rft.format=text&rft.identifier=http%3A%2F%2Fdigilib.unila.ac.id%2F33171%2F2%2FSKRIPSI%2520FULL.pdf&rft.format=text&rft.identifier=http%3A%2F%2Fdigilib.unila.ac.id%2F33171%2F3%2FSKRIPSI%2520TANPA%2520BAB%2520PEMBAHASAN.pdf&rft.identifier=++NI+PUTU+RAHMA+AGUSTINA%2C+1417011080++(2018)+KONVERSI+ENZIMATIS+PATI+SAGU+MENJADI+GLUKOSA+MENGGUNAKAN+ENZIM+%EF%81%A1-AMILASE+DARI+Bacillus+Subtilis+ITBCCB148+YANG+DIAMOBILISASI+DENGAN+KITOSAN+UNTUK+PRODUKSI+BIOETANOL.++FAKULTAS+MATEMATIKA+DAN+ILMU+PENGETAHUAN+ALAM+%2C+UNIVERSITAS+LAMPUNG.+++++&rft.relation=http%3A%2F%2Fdigilib.unila.ac.id%2F33171%2F