?url_ver=Z39.88-2004&rft_id=1417011094&rft_val_fmt=info%3Aofi%2Ffmt%3Akev%3Amtx%3Adc&rft.title=KONVERSI+ENZIMATIS+PATI+ONGGOK+MENJADI+GLUKOSA+MENGGUNAKAN+ENZIM+a-AMILASE+DARI+Bacillus+subtilis+ITBCCB148+YANG+DIAMOBILISASI+DENGAN+ZEOLIT+ALAM+UNTUK+PRODUKSI+BIOETANOL&rft.creator=Riza+Mufarida+Akhsin%2C+1417011094&rft.subject=+&rft.subject=QD+Chemistry&rft.description=Kebutuhan+terhadap+energi+semakin+meningkat+namun+ketersediaan+bahan+bakar+fosil+semakin+menurun.++Energi+terbarukan+dibutuhkan+sebagai+sumber+alternatif%2C+salah+satunya+adalah+bioetanol.++Penelitian+mengenai+produksi+bioetanol+oleh+enzim+sangatlah+dibutuhkan.++Penelitian+ini+bertujuan+untuk+mengetahui+pengaruh+amobilisasi+terhadap+kestabilan+enzim.++Enzim+%CE%B1-amilase+hasil+amobilisasi+digunakan+untuk+mengonversi+pati+onggok+menjadi+glukosa+untuk+produksi+bioetanol.++Tahap+penelitian+ini+meliputi+proses+produksi%2C+isolasi%2C+pemurnian%2C+amobilisasi%2C+karakterisasi%2C+konversi+enzimatis%2C+dan+fermentasi.++Hasil+penelitian+menunjukkan+aktivitas+spesifik+enzim+hasil+pemurnian+sebesar+10.318%2C898+U%2Fmg+dan+kemurniannya+meningkat+13+kali+dibandingkan+ekstrak+kasarnya.++Enzim+hasil+pemurnian+memiliki+suhu+optimum+55oC%2C+KM+%3D+7%2C31+mg%2FmL+substrat%2C+Vmaks+%3D+90%2C91+%C2%B5mol%2FmL.menit%2C+sedangkan+enzim+hasil+amobilisasi+memiliki+suhu+optimum+70oC%2C+KM+%3D+14%2C78+mg%2FmL+substrat%2C+Vmaks+%3D+36%2C9+%C2%B5mol%2FmL.menit.++Aktivitas+sisa+dari+enzim+hasil+pemurnian+dan+hasil+amobilisasi+pada+uji+stabilitas+termal+berturut-turut+sebesar+18+dan+87%25.++Data+kinetika+enzim+hasil+pemurnian+diperoleh+nilai+ki+%3D+0%2C0226+menit-1%2C+%E2%88%86Gi+%3D+92%2C364+kJ%2Fmol%2C+dan+t1%2F2+%3D+30%2C664+menit%2C+sedangkan+enzim+hasil+amobilisasi+diperoleh+nilai+ki+%3D+0%2C0013+menit-1%2C+%E2%88%86Gi+%3D+111%2C607+kJ%2Fmol%2C+dan+t1%2F2+%3D+533%2C077+menit.++Data+tersebut+menunjukkan+bahwa+amobilisasi+dengan+zeolit+alam+dapat+meningkatkan+kestabilan+enzim.++Kadar+bioetanol+yang+diperoleh+dari+proses+fermentasi+menggunakan+Saccharomyces+sereviciae+dan+ragi+berturut-turut+sebesar+0%2C14+dan+0%2C30%25.%0D%0A%0D%0A%0D%0AKata+kunci+%3A+%CE%B1-amilase%2C+Bacillus+subtilis+ITBCCB148%2C+amobilisasi%2C+zeolit+alam%2C+pati+onggok%2C+bioetanol.%0D%0A%0D%0A%0D%0A%0D%0AThe+demand+of+fuel+energy+are+increasing+time+by+time%2C+but+the+stock+of+fossil+fuel+are+decreasing.++Nowadays+renewable+energy+as+an+alternative+sources+are+needed%2C+such+as+bioethanol.++Study+about+bioethanol+production+including+the+enzyme+systems+are+required.++The+objective+of+this+study+is+to+determine+the+effect+of+immobilization+on+the+stability+of+enzymes.++The+immobilized+of+%CE%B1-amylase+enzyme+is+used+to+convert+onggok+starch+into+glucose+for+bioethanol+production.++The+steps+of+this+study+includes+production+process%2C+isolation%2C+purification%2C+immobilization%2C+characterization%2C+enzymatic+conversion%2C+and+fermentation.++Our+observation+showed+that+the+specific+activity+of+purified+enzyme+by+dialysis+was+10%2C318.898+U%2Fmg+and+its+purity+increased+13+times+than+the+crude+ones.++The+purified+enzyme+has+an+optimum+temperature+of+55oC%2C+KM+%3D+7.31+mg%2FmL+substrate%2C+Vmax+%3D+90.91+%CE%BCmol%2FmL.min%2C+moreover+the+immobilized+enzyme+has+an+optimum+temperature+of+70oC%2C+KM+%3D+14.78+mg%2FmL+substrate%2C+Vmax+%3D+36.9+%CE%BCmol%2FmL.min.++The+residual+activity+of+the+purified+and+immobilized+enzyme+on+thermal+stability+were+18+and+87%25+respectively.++The+kinetic+study+of+the+purified+enzyme+obtained+ki+%3D+0.0226+min-1%2C+%CE%94Gi+%3D+92.364+kJ%2Fmol%2C+and+t1%2F2+%3D+30.664+min%2C+moreover+the+immobilized+enzyme+obtained+ki+%3D+0.0013+min-1%2C+%CE%94Gi+%3D+111.607+kJ%2Fmol%2C+and+t1%2F2+%3D+533.077+minutes.++Our+Investigation+suggests+that+immobilization+with+natural+zeolite+can+improve+the+stability+of+enzymes.++The+bioethanol+that+obtained+from+fermentation+process+using+Saccharomyces+sereviciae+and+yeast+were+0.14+and+0.30%25+respectively.%0D%0A%0D%0A%0D%0AKeywords+%3A+%CE%B1-amylase%2C+Bacillus+subtilis+ITBCCB148%2C+immobilization%2C+natural+zeolite%2C+onggok+starch%2C+bioethanol.&rft.publisher=Fakultas+Matematika+dan+Ilmu+Pengetahuan+Alam&rft.date=2018-08-31&rft.type=Skripsi&rft.type=NonPeerReviewed&rft.format=text&rft.identifier=http%3A%2F%2Fdigilib.unila.ac.id%2F37181%2F1%2FABSTRAK.pdf&rft.format=text&rft.identifier=http%3A%2F%2Fdigilib.unila.ac.id%2F37181%2F2%2FSKRIPSI%2520FULL.pdf&rft.format=text&rft.identifier=http%3A%2F%2Fdigilib.unila.ac.id%2F37181%2F3%2FSKRIPSI%2520TANPA%2520BAB%2520PEMBAHASAN.pdf&rft.identifier=++Riza+Mufarida+Akhsin%2C+1417011094++(2018)+KONVERSI+ENZIMATIS+PATI+ONGGOK+MENJADI+GLUKOSA+MENGGUNAKAN+ENZIM+a-AMILASE+DARI+Bacillus+subtilis+ITBCCB148+YANG+DIAMOBILISASI+DENGAN+ZEOLIT+ALAM+UNTUK+PRODUKSI+BIOETANOL.++Fakultas+Matematika+dan+Ilmu+Pengetahuan+Alam%2C+Universitas+Lampung.+++++&rft.relation=http%3A%2F%2Fdigilib.unila.ac.id%2F37181%2F