?url_ver=Z39.88-2004&rft_id=1417011018&rft_val_fmt=info%3Aofi%2Ffmt%3Akev%3Amtx%3Adc&rft.title=KONVERSI+ENZIMATIS+PATI+ONGGOK+MENJADI+GLUKOSA+MENGGUNAKAN+ENZIM+%CE%B1-AMILASE+DARI+Bacillus+subtilis+ITBCCB148+YANG+DIAMOBILISASI+DENGAN+BENTONIT+UNTUK+PRODUKSI+BIOETANOL&rft.creator=BUNGA+LANTRI+DWINTA%2C+1417011018&rft.subject=QD+Chemistry&rft.description=Amilase+banyak+digunakan+secara+komersial+dalam+produk+industri+pangan+dan+non+pangan.++Salah+satunya+adalah+produk+bioetanol%2C+maka+enzim+yang+digunakan+sebaiknya+memiliki+stabilitas+enzim+yang+tinggi+agar+dapat+bekerja+pada+pH+dan+suhu+ekstrim.++Penelitian+ini+bertujuan+untuk+mengetahui+pengaruh+amobilisasi+enzim+pada+matriks+pengamobil+yaitu+bentonit+terhadap+kestabilan+enzim.++Enzim+amobil+kemudian+digunakan+untuk+mengonversi+substrat+pati+onggok+menjadi+glukosa+untuk+produksi+bioetanol.++Tahap+penelitian+ini+meliputi+proses+produksi%2C+isolasi%2C+pemurnian+enzim%2C+amobilisasi+enzim%2C+karakterisasi+enzim%2C+konversi+enzimatis%2C+dan+fermentasi.++Hasil+penelitian+menunjukkan+aktivitas+spesifik+enzim+murni+sebesar+22.388%2C82+U%2Fmg+dan+kemurniannya+meningkat+32%2C61+kali+dibandingkan+ekstrak+kasar+enzim.++Enzim+murni+memiliki+suhu+optimum+55%C2%BAC%2C+KM+%3D+4%2C8+mg%2FmL+substrat%2C+dan+Vmaks+%3D+50+%CE%BCmol+mL-1+menit-1%2C+sedangkan+enzim+amobil+memiliki+suhu+optimum+60+%C2%BAC%2C+KM+%3D+9%2C81+mg%2FmL+substrat%2C+dan+Vmaks+%3D+34%2C01+%CE%BCmol+mL-1+menit-1.++Aktivitas+sisa+dari+enzim+murni+dan+enzim+amobil+pada+uji+stabilitas+termal+berturut-turut+adalah+28+dan+61%25.++Data+kinetika+enzim+murni+diperoleh+nilai+ki+%3D+0%2C014+menit-1%2C+%CE%94Gi+%3D+98%2C645+kJ+mol-1%2C+dan+t1%2F2+%3D+49%2C5+menit%2C+sedangkan+enzim+amobil+diperoleh+nilai+ki+%3D+0%2C005+menit-1%2C+%CE%94Gi+%3D+107%2C876+kJ+mol-1%2C+dan+t1%2F2+%3D+138%2C6+menit%2C+dari+data+tersebut+menunjukkan+bahwa+amobilisasi+dengan+bentonit+dapat+meningkatkan+kestabilan+enzim.++Kadar+bioetanol+yang+diperoleh+dari+proses+fermentasi+menggunakan+S.+cereviciae+dan+ragi+berturut-turut+adalah+0%2C215+dan+0%2C247%25.++%0D%0A%0D%0A%0D%0AKata+kunci+%3A+%CE%B1-amilase%2C+amobilisasi+enzim%2C+Bacillus+subtilis+ITBCCB148%2C+bentonit%2C+bioetanol%2C+pati+onggok.%0D%0A%0D%0A%0D%0AAmylase+is+widely+apply+commercially+in+food+and+non-food+industry.+One+of+them+is+in+bioethanol+production+the+required-enzyme+should+have+high+stability+and+also+stable+on+pH+and+extreme+temperature.++This+study+explored+the+effect+of+amylase+immobilization+on+bentonite+matrix+to+have+high+stability+that+required+to+convert+the+starch+substrate+into+glucose+in+a+part+of+bioethanol+production.++The+steps+of+this+study+includes+the+production+process%2C+isolation%2C+purification%2C+immobilization%2C+characterization%2C+enzymatic+conversion%2C+and+fermentation.++Our+investigation+showed+that+the+specific+activity+of+purified+enzyme+was+22%2C388.82+U%2Fmg+and+its+purity+increased+32.61+times+than+crude+extract+ones.++Purified+enzyme+has+an+optimum+temperature+of+55%C2%BAC%2C+KM+%3D+4.8+mg%2FmL+substrate%2C+and+Vmax+%3D+50+%CE%BCmol+mL-1+min-1%2C+in+addition+the+immobilized+enzyme+has+an+optimum+temperature+of+60+%C2%BAC%2C+KM+%3D+9.81+mg%2FmL+substrate%2C+and+Vmax+%3D+34.01+%CE%BCmol+mL-1+min-1.++The+residual+activity+of+purified+and+immobilized+enzymes+in+the+thermal+stability+test+were+28+and+61%25.++From+data+of+purified+enzyme+kinetics+showed+that+the+value+of+ki+%3D+0.014+min-1%2C+%CE%94Gi+%3D+98.645+kJ+mol-1%2C+and+t1%2F2+%3D+49.5+min%2C+in+addition+the+immobilized+enzyme+has+ki+%3D+0.005+min-1%2C+%CE%94Gi+%3D+107.876+kJ+mol-1%2C+and+t1%2F2+%3D+138.6+min+respectively.++Our+investigation+showed+that+immobilization+bentonite-immobilized+amylase+has+higher+stability+than+the+pure+one.++Bioethanol+obtained+from+fermentation+process+using+S.cereviciae+and+yeast+were+0.215+and+0.247%25+respectively.%0D%0A%0D%0AKeywords%3A+%CE%B1-amylase%2C+enzyme+immobilization%2C+Bacillus+subtilis+ITBCCB148%2C+bentonite%2C+bioethanol%2C+onggok+starch.%0D%0A&rft.publisher=Fakultas+Matematika+dan+Ilmu+Pengetahuan+Alam&rft.date=2018-09-12&rft.type=Skripsi&rft.type=NonPeerReviewed&rft.format=text&rft.identifier=http%3A%2F%2Fdigilib.unila.ac.id%2F37184%2F1%2FABSTRAK%2520%2528ABSTRACT%2529.pdf&rft.format=text&rft.identifier=http%3A%2F%2Fdigilib.unila.ac.id%2F37184%2F2%2FSKRIPSI%2520FULL.pdf&rft.format=text&rft.identifier=http%3A%2F%2Fdigilib.unila.ac.id%2F37184%2F3%2FSKRIPSI%2520TANPA%2520BAB%2520PEMBAHASAN.pdf&rft.identifier=++BUNGA+LANTRI+DWINTA%2C+1417011018++(2018)+KONVERSI+ENZIMATIS+PATI+ONGGOK+MENJADI+GLUKOSA+MENGGUNAKAN+ENZIM+%CE%B1-AMILASE+DARI+Bacillus+subtilis+ITBCCB148+YANG+DIAMOBILISASI+DENGAN+BENTONIT+UNTUK+PRODUKSI+BIOETANOL.++Fakultas+Matematika+dan+Ilmu+Pengetahuan+Alam%2C+Universitas+Lampung.+++++&rft.relation=http%3A%2F%2Fdigilib.unila.ac.id%2F37184%2F