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PROFIL NITRAT ANORGANIK DAN PROTEIN TOTAL INTRASELULER PADA FASE EKSPONENSIAL BIOMASSA Nannochloropsis sp.
B.INGGRIS Inorganic nitrate is one of the important sources of nutrients in the culture of Nannochloropsis sp. especially in exponential phase, and factor of fluctuations in cell density and intracellular crude protein content. The study aimed to analyze the effect of reduction inorganic nitrate up to 50% in Conwy culture media towards density and intracellular crude protein content Nannochloropsis sp. in exponential phase. The results showed that reduction of inorganic nitrate content can shorten exponential phase so that can accelerate harvest microalgae. Reduction of inorganic nitrate also has a negative side, which was a decrease of intracellular crude protein content and cell density Nannochloropsis sp. Deficiency of inorganic nitrate by 50% inclined to reduce 19,3% of P/Nt ratio and 3,26% of P/NO3- ratio. Keywords: inorganic nitrate, exponential phase, intracellular crude protein, Nannochloropsis sp., cell density B.INDONESIA Nitrat anorganik merupakan salah satu sumber nutrien penting dalam kultur Nannochloropsis sp. terutama pada fase eksponensial, dan faktor penentu fluktuasi kepadatan sel dan kandungan protein total intraseluler. Penelitian bertujuan menganalisis pengaruh pengurangan nitrat anorganik sebanyak 50% pada pupuk Conwy terhadap kepadatan dan kandungan protein total intraseluler Nannochloropsis sp. pada fase eksponensial. Hasil penelitian menunjukkan bahwa pengurangan kandungan nitrat anorganik dapat memperpendek fase eksponensial sehingga dapat mempecepat waktu panen mikroalga. Pengurangan nitrat anorganik juga memiliki sisi negatif yaitu penurunan kandungan protein total intraseluler dan kepadatan sel Nannochloropsis sp. Defisiensi nitrat anorganik sebesar 50% cenderung akan menurunkan rasio P/Nt sebesar 19,3% dan menurunkan rasio P/NO3- sebesar 3,26%. Kata kunci: nitrat anorganik, protein total intraseluler, fase eksponensial, Nannochloropsis sp., kepadatan.
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